TIGIT (NM_173799) Human Mass Spec Standard

CAT#: PH321447

TIGIT MS Standard C13 and N15-labeled recombinant protein (NP_776160)

Product Images

Coomassie blue staining of purified TIGIT protein (Cat# TP321447). The protein was produced from HEK293T cells transfected with TIGIT cDNA clone (Cat# RC221447) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC221447
• Predicted MW: 26.3 kDa
• Protein Sequence:
  Sequence Link

  >RC221447 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRWCLLLIWAQGLRQAPLASGMMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICN
  ADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQI
  PLLGAMAATLVVICTAVIVVVALTRKKKALRIHSVEGDLRRKSAGQEEWSPSAPSPPGSCVQAEAAPAGL
  CGEQRGEDCAELHDYFNVLSYRSLGNCSFFTETG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_776160
• RefSeq Size: 2978
• RefSeq ORF: 732
• Synonyms: VSIG9; VSTM3; WUCAM
• Locus ID: 201633
• UniProt ID: Q495A1
• Cytogenetics: 3q13.31
• Summary: This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.[provided by RefSeq, Sep 2009]
• Protein Families: Transmembrane