Nucleolin (NCL) (NM_005381) Human Mass Spec Standard

SKU
PH319082
NCL MS Standard C13 and N15-labeled recombinant protein (NP_005372)
  $3,360.00
3 Weeks*
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Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC219082
Predicted MW 76.4 kDa
Protein Sequence
Protein Sequence
RC219082
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_005372
RefSeq Size 2732
RefSeq ORF 2130
Synonyms C23; Nsr1
Locus ID 4691
UniProt ID P19338
Cytogenetics 2q37.1
Summary Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. provided by RefSeq, Jul 2008
Protein Families Druggable Genome, Stem cell - Pluripotency
Protein Pathways Pathogenic Escherichia coli infection
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Other "Nucleolin" proteins (3)
SKU Description Size Price
LC417337 Lyophilized NCL HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY417337 Transient overexpression lysate of nucleolin (NCL) (as WB positive control) 100 ug
$665.00
TP319082 Recombinant protein of human nucleolin (NCL), 20 µg 20 ug
$867.00

file-alt Citations

Aberrant expression of CITED2 promotes prostate cancer metastasis by activating the nucleolin-AKT pathway
Shin, Lee, Lee et al
Nat Commun (2018) 9 (1), 4113 DOI: 10.1038/s41467-018-06606-2
AI Summary: OriGene contributed to this study by providing human recombinant proteins of CITED2, PRMT5, P300, and NCL, which were used to investigate the molecular mechanisms underlying prostate cancer metastasis.
Snippet: anti-p-AKT, anti-AKT and anti-E-cadherin from Cell Signaling (Danvers, MA);anti-FLAG, anti-MYC, and anti-HA from Sigma-Aldrich (St. Louis, MO);and anti-ERG and anti-α-SMA from Abcam (Cambridge, MA). MK2206 was purchased from Selleckchem. Human recombinant proteins of CITED2, PRMT5, P300, and NCL were purchased from Origene
FigureFigureFigure
Showing 1-1 of 1 papers.
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