PRAMEF19 (NM_001099790) Human Mass Spec Standard

CAT#: PH313704

PRAMEF19 MS Standard C13 and N15-labeled recombinant protein (NP_001093260)

Product Images

Coomassie blue staining of purified PRAMEF19 protein (Cat# TP313704). The protein was produced from HEK293T cells transfected with PRAMEF19 cDNA clone (Cat# RC213704) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC213704
• Predicted MW: 55.1 kDa
• Protein Sequence:
  Sequence Link

  >RC213704 representing NM_001099790
  Red=Cloning site Green=Tags(s)
  
  MSFQAPRRLLELAGQSLLRDQALAISVLDELPRELFPRLFVEAFTSRRCEVLKVMVQAWPFPCLPLGSLM
  KTPDLEILHYVVDGIDCLLAQKVRPRRWKLQVLEMRDVDENFWTIWSGARPLSCSPEAMSKRQTVEDCPR
  TGEKQPLKVFMDVCLKEKSVDEDLSFFSGWVQHRRRSVHLCCTKVVNYSMNILNFRNILETVYPDSIQVL
  EIWNMCWPCMVAEVSRYLSQMKNLRKLFISDGCGYLPSFESQGQLVAEFSSVFLRLEYLQMLYMRRIRFF
  EGYLDQLIRCLKSPLETLALTYGSLDEEDLKCLPWYPSLSQLKQLNLSHGTLRFIRLEPLRALLEKVAAT
  LQTLFLVDCGIGYSKLRVILPALSRCSNLTTFCFHGNDTSMDGLKDLLRHTGRLSNLSLETYPAPRESLD
  NRGRVISELLTPLQAELMRILREVREPNRIFFGPVSCPCCGMSPTEQLEFNFCLRGRPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001093260
• RefSeq Size: 2123
• RefSeq ORF: 1437
• Locus ID: 645414
• UniProt ID: Q5SWL8
• Cytogenetics: 1p36.21