OLAH (NM_001039702) Human Mass Spec Standard

CAT#: PH308251

OLAH MS Standard C13 and N15-labeled recombinant protein (NP_001034791)

Product Images

Coomassie blue staining of purified OLAH protein (Cat# TP308251). The protein was produced from HEK293T cells transfected with OLAH cDNA clone (Cat# RC208251) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC208251
• Predicted MW: 29.9 kDa
• Protein Sequence:
  Sequence Link

  >RC208251 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERGDQPKRTRNENIFNCLYKNPEATFKLICFPWMGGGSTHFAKWGQDTHDLLEVHSLRLPGRESRVEEP
  LENDISQLVDEVVCALQPVIQDKPFAFFGHSMGSYIAFRTALGLKENNQPEPLHLFLSSATPVHSKAWHR
  IPKDDELSEEQISHYLMEFGGTPKHFAEAKEFVKQCSPIIRADLNIVRSCTSNVPSKAVLSCDLTCFVGS
  EDIAKDMEAWKDVTSGNAKIYQLPGGHFYLLDPANEKLIKNYIIKCLEVSSISNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_001034791
• RefSeq Size: 1675
• RefSeq ORF: 795
• Synonyms: AURA1; SAST; TE2; THEDC1
• Locus ID: 55301
• UniProt ID: Q9NV23
• Cytogenetics: 10p13
• Summary: Contributes to the release of free fatty acids from fatty acid synthase (FASN). Has broad substrate specificity, giving rise to a range of free fatty acids with chain lengths between 10 and 16 carbon atoms (C10 - C16).[UniProtKB/Swiss-Prot Function]
• Protein Pathways: Fatty acid biosynthesis, Metabolic pathways