EFHB (NM_144715) Human Mass Spec Standard

CAT#: PH307068

EFHB MS Standard C13 and N15-labeled recombinant protein (NP_653316)

Product Images

Coomassie blue staining of purified EFHB protein (Cat# TP307068). The protein was produced from HEK293T cells transfected with EFHB cDNA clone (Cat# RC207068) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC207068
• Predicted MW: 76.4 kDa
• Protein Sequence:
  Sequence Link

  >RC207068 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPRQEMEKESTCVLMKPNTEIKLPVEVDIGLTQAEGPDETKNTEPQMGLVIEPPQCQFAQQHEQRKEAG
  NIESGVEPPDRIRPIYSGKFFDRTPCWPSAGKVIPVGYRVATCLTEKLPRLITPPEAKKYFNFRYPPAGV
  ERVFYGRANDPQIAPYLTHGIRSKISILANTLINPQPITTFQQKIKDKKESIYLSNRRAPLGKSHDQAPG
  LPKGMDTINTTFGTAVIKEYSAKDVVNPPKSYEEVFKEGNEGHDLYVVSHNDYYAGEAKNRKYNPSSFHR
  CSVYGVPTPHFNDGRAMAKSLYWLHELQMKRGAKFVSKRADDFKEKFQHKLGRVLDPIAETMNVPPDCTF
  GACLRPEEYGVGDLIHNRLPDEYLRGKDRQRALIAAVRHHLKKVNYQKFDTLLAAFRHYDKKGDGMIDKD
  ELQEACDQANLSLDDKLLDQLFDYCDVDNDGFINYLEFANFLNWKDKMLLKEYEERVIIKGRKPDCVNPT
  EANVEEPEQTLLIKPEDIVLKEAGSTEKTLRTLLRPSDKVSNYYKTTSSEINAIVGAIPSTCYPICGVPT
  IRSDIPAPRIRRISDRTNYGEEGSAYSLLYPTIFARKGVFERDFFKTRSKEEIAEILCNIGVKLSDEEFE
  NVWNLASKKHHRGEVCVENIRNVLDELRHADRIKCKTLM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_653316
• RefSeq Size: 2857
• RefSeq ORF: 2007
• Synonyms: CFAP21
• Locus ID: 151651
• UniProt ID: Q8N7U6
• Cytogenetics: 3p24.3
• Summary: Cytosolic sensor for calcium, modulates the interaction of STIM1 and ORAI1 upon store depletion, the activation of store-operated Ca(2+) entry (SOCE) and NFAT translocation from cytosol to nucleus.[UniProtKB/Swiss-Prot Function]