DUSP28 (NM_001033575) Human Mass Spec Standard

SKU
PH306912
DUSP28 MS Standard C13 and N15-labeled recombinant protein (NP_001028747)
  $3,360.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC206912
Predicted MW 18.3 kDa
Protein Sequence
Protein Sequence
>RC206912 protein sequence
Red=Cloning site Green=Tags(s)

MGPAEAGRRGAASPVPPPLVRVAPSLFLGSARAAGAEEQLARAGVTLCVNVSRQQPGPRAPGVAELRVPV
FDDPAEDLLAHLEPTCAAMEAAVRAGGACLVYCKNGRSRSAAVCTAYLMRHRGLSLAKAFQMVKSARPVA
EPNPGFWSQLQKYEEALQAQSCLQGEPPALGLGPEA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_001028747
RefSeq Size 1555
RefSeq ORF 528
Synonyms DUSP26; VHP
Locus ID 285193
UniProt ID Q4G0W2
Cytogenetics 2q37.3
Summary Has phosphatase activity with the synthetic substrate 6,8-difluoro-4-methylumbelliferyl phosphate (in vitro) (PubMed:24531476, PubMed:29121083). Has almost no detectable activity with phosphotyrosine, even less activity with phosphothreonine and displays complete lack of activity with phosphoserine (PubMed:29121083). The poor activity with phosphotyrosine may be due to steric hindrance by bulky amino acid sidechains that obstruct access to the active site (PubMed:29121083).UniProtKB/Swiss-Prot Function
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Other "DUSP28" proteins (3)
SKU Description Size Price
LC422400 Lyophilized DUSP28 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY422400 Transient overexpression lysate of dual specificity phosphatase 28 (DUSP28) (as WB positive control) 100 ug
$436.00
TP306912 Recombinant protein of human dual specificity phosphatase 28 (DUSP28), 20 µg 20 ug
$737.00

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