MRPL32 (NM_031903) Human Mass Spec Standard
CAT#: PH304559
MRPL32 MS Standard C13 and N15-labeled recombinant protein (NP_114109)
Specifications
Product Data | |
Tag | C-Myc/DDK |
Species | Human |
Expression Host | HEK293 |
Expression cDNA Clone or AA Sequence | RC204559 |
Predicted MW | 21.4 kDa |
Protein Sequence |
>RC204559 protein sequence
Red=Cloning site Green=Tags(s) MALAMLVLVVSPWSAARGVLRNYWERLLRKLPQSRPGFPSPPWGPALAVQGPAMFTEPANDTSGSKENSS LLDSIFWMAAPKNRRTIEVNRCRRRNPQKLIKVKNNIDVCPECGHLKQKHVLCAYCYEKVCKETAEIRRQ IGKQEGGPFKAPTIETVVLYTGETPSEQDQGKRIIERDRKRPSWFTQN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Purity | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Concentration | >0.05 µg/µL as determined by microplate BCA method |
Labeling Method | Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine |
Buffer | 25 mM Tris-HCl, 100 mM glycine, pH 7.3 |
Storage | Store at -80°C. Avoid repeated freeze-thaw cycles. |
Stability | Stable for 3 months from receipt of products under proper storage and handling conditions. |
Reference Data | |
RefSeq | NP_114109 |
RefSeq Size | 908 |
RefSeq ORF | 564 |
Synonyms | bMRP-59b; HSPC283; L32mt; MRP-L32 |
Locus ID | 64983 |
UniProt ID | Q9BYC8, A4D1V4 |
Cytogenetics | 7p14.1 |
Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq, Jul 2008] |
Documents
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC410450 | MRPL32 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 134.00 |
|
LY410450 | Transient overexpression lysate of mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein |
USD 436.00 |
|
TP304559 | Recombinant protein of human mitochondrial ribosomal protein L32 (MRPL32), nuclear gene encoding mitochondrial protein, 20 µg |
USD 867.00 |
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