SCLY (NM_016510) Human Mass Spec Standard

CAT#: PH303164

SCLY MS Standard C13 and N15-labeled recombinant protein (NP_057594)

Product Images

Coomassie blue staining of purified SCLY protein (Cat# TP303164). The protein was produced from HEK293T cells transfected with SCLY cDNA clone (Cat# RC203164) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203164
• Predicted MW: 48.2 kDa
• Protein Sequence:
  Sequence Link

  >RC203164 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEAAVAPGRDAPAPAASQPSGCGKHNSPERKVYMDYNATTPLEPEVIQAMTKAMWEAWGNPSSPYSAGRK
  AKDIINAARESLAKMIGGKPQDIIFTSGGTESNNLVIHSVVKHFHANQTSKGHTGGHHSPVKGAKPHFIT
  SSVEHDSIRLPLEHLVEEQVAAVTFVPVSKVSGQTEVDDILAAVRPTTRLVTIMLANNETGIVMPVPEIS
  QRIKALNQERVAAGLPPILVHTDAAQALGKQRVDVEDLGVDFLTIVGHKFYGPRIGALYIRGLGEFTPLY
  PMLFGGGQERNFRPGTENTPMIAGLGKAAELVTQNCEAYEAHMRDVRDYLEERLEAEFGQKRIHLNSQFP
  GTQRLPNTCNFSIRGPRLQGHVVLAQCRVLMASVGAACHSDHGDQPSPVLLSYGVPFDVARNALRLSVGR
  STTRAEVDLVVQDLKQAVAQLEDQA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_057594
• RefSeq Size: 2544
• RefSeq ORF: 1335
• Synonyms: hSCL; SCL
• Locus ID: 51540
• UniProt ID: Q96I15, B4DDP9, A0A0A0MQU4, Q59FK2
• Cytogenetics: 2q37.3
• Summary: Selenocysteine lyase (SCLY; EC 4.4.1.16) catalyzes the pyridoxal 5-prime phosphate-dependent conversion of L-selenocysteine to L-alanine and elemental selenium (Mihara et al., 2000 [PubMed 10692412]).[supplied by OMIM, Mar 2008]
• Protein Pathways: Metabolic pathways, Selenoamino acid metabolism