PPIH (NM_006347) Human Mass Spec Standard

CAT#: PH301529

PPIH MS Standard C13 and N15-labeled recombinant protein (NP_006338)

Product Images

Coomassie blue staining of purified PPIH protein (Cat# TP301529). The protein was produced from HEK293T cells transfected with PPIH cDNA clone (Cat# RC201529) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC201529
• Predicted MW: 19.2 kDa
• Protein Sequence:
  Sequence Link

  >RC201529 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAVANSSPVNPVVFFDVSIGGQEVGRMKIELFADVVPKTAENFRQFCTGEFRKDGVPIGYKGSTFHRVIK
  DFMIQGGDFVNGDGTGVASIYRGPFADENFKLRHSAPGLLSMANSGPSTNGCQFFITCSKCDWLDGKHVV
  FGKIIDGLLVMRKIENVPTGPNNKPKLPVVISQCGEM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006338
• RefSeq Size: 813
• RefSeq ORF: 531
• Synonyms: CYP-20; CYPH; SnuCyp-20; USA-CYP
• Locus ID: 10465
• UniProt ID: O43447, Q6FH36
• Cytogenetics: 1p34.2
• Summary: The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq, Jul 2008]
• Protein Pathways: Spliceosome