NTAQ1 (NM_018024) Human Mass Spec Standard

SKU
PH301055
WDYHV1 MS Standard C13 and N15-labeled recombinant protein (NP_060494)
  $3,360.00
3 Weeks*
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Specifications
Specifications
Product Data
Tag C-Myc/DDK
Species Human
Expression Host HEK293
Expression cDNA Clone or AA Sequence RC201055
Predicted MW 23.6 kDa
Protein Sequence
Protein Sequence
>RC201055 protein sequence
Red=Cloning site Green=Tags(s)

MEGNGPAAVHYQPASPPRDACVYSSCYCEENVWKLCEYIKNHDQYPLEECYAVFISNERKMIPIWKQQAR
PGDGPVIWDYHVVLLHVSSGGQSFIYDLDTVLPFPCLFDTYVEDAIKSDDDIHPQFRRKFRVICADSYLK
NFASDRSHMKDSSGNWREPPPPYPCIETGDSKMNLNDFISMDPKVGWGAVYTLSEFTHRFGSKNC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration >0.05 µg/µL as determined by microplate BCA method
Labeling Method Labeled with U- 13C6, 15N4-L-Arginine and U- 13C6, 15N2-L-Lysine
Buffer 25 mM Tris-HCl, 100 mM glycine, pH 7.3
Storage Store at -80°C. Avoid repeated freeze-thaw cycles.
Stability Stable for 3 months from receipt of products under proper storage and handling conditions.
Shipping Dry Ice
Reference Data
RefSeq NP_060494
RefSeq Size 1568
RefSeq ORF 615
Synonyms C8orf32; WDYHV1
Locus ID 55093
UniProt ID Q96HA8
Cytogenetics 8q24.13
Summary Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine.UniProtKB/Swiss-Prot Function
Protein Families Stem cell - Pluripotency
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Other "NTAQ1" proteins (3)
SKU Description Size Price
LC402638 Lyophilized WDYHV1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY402638 Transient overexpression lysate of WDYHV motif containing 1 (WDYHV1) (as WB positive control) 100 ug
$436.00
TP301055 Recombinant protein of human WDYHV motif containing 1 (WDYHV1), 20 µg 20 ug
$867.00

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