S100A1 (NM_006271) Human Mass Spec Standard

CAT#: PH300278

S100A1 MS Standard C13 and N15-labeled recombinant protein (NP_006262)

Product Images

Coomassie blue staining of purified S100A1 protein (Cat# TP300278). The protein was produced from HEK293T cells transfected with S100A1 cDNA clone (Cat# RC200278) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200278
• Predicted MW: 10.5 kDa
• Protein Sequence:
  Sequence Link

  >RC200278 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSELETAMETLINVFHAHSGKEGDKYKLSKKELKELLQTELSGFLDAQKDVDAVDKVMKELDENGDGEV
  DFQEYVVLVAALTVACNNFFWENS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_006262
• RefSeq Size: 607
• RefSeq ORF: 282
• Synonyms: S100; S100-alpha; S100A
• Locus ID: 6271
• UniProt ID: P23297, A0A0S2Z4H2
• Cytogenetics: 1q21.3
• Summary: The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008]