Lentiviral Control Particles

Snippet: Oligodendrogliomas (ODGs) are slow-growing tumors with poorly defined biology. Here, we identify SMPD3, encoding neutral sphingomyelinase 2 (nSMase2), as a;To knock down SMPD3, BT88 and BT54 cells were transduced with four human shRNA lentiviral constructs (A–D;pGFP-c-shLenti-shSMPD3A-D, TL301492VA-D;OriGene) or scrambled shRNA control particles (pGFP-C-shLenti- shScrambled, TR30021V;OriGene)

Snippet: 2 weeks.Lentiviral delivery and stereotaxic injectionsTo knock down glycan biosynthesis, short hairpin RNA targeting PGM3 (shPGM3, TL505905V;Origene) or control shScrambled lentivirus (TR30021V;Origene) was bilaterally injected into the lateral ventricles of WT, 5xFAD and PS19 mice. Injections were performed using a stereotaxic frame at c

Snippet: thal dose for untransduced cells was selected (Supplementary Fig. 3). Commercially available lentiviral particles targeting NAALADL1 (Catalog No. TL311278V, OriGene Technologies GmbH, Herford, Germany;A-D mix in equal volumes) and corresponding control vectors (Catalog No. TR30021V, OriGene Technologies GmbH), which contain random sequences of the same l

Snippet: plasmidKIF11 human shRNA lentiviral particles (Locus ID 3832, Catalog Number: TL311921V) and their corresponding control lentiviral particles (Catalog Number: TR30021V) were obtained from Origene Inc. … The transduction of both KIF11 shRNA and control sh;- Number: TR30021V) were obtained from Origene Inc. …

Snippet: ) at a dose of 1 × 10^8 transducing units (TU) per mouse, administered 2 days prior to OVA treatment;lv-sh-NC (Cat# TR30021V, OriGene) at the same viral titre served as;× 10^5 cells per well in 6‐well plates and allowed to adhere overnight. Upon reaching approximately 60%–70% confluency, cells were transfected with si‐KIF1B (1:1000 dilution, Cat# TL303695, OriGene, using si‐NC as the control) or NLRP3 ORF‐clone (1:1000 dilution, Cat# RC223212, OriGene, using empty vector as the control) using Lipofectamine 3000 reagent according to the manufacturer'