Lenti Concentrator

Snippet: id and packaging constructs (PMDL, REV, VSVG) using calcium chloride transfection. Viral supernatant was collected at 48 and 72 hours post-transfection, concentrated using 5X Lentiviral Concentrator (Origene, Cat. #TR30026) and stored at −80°C.To generate PyMT-CK(OB) and PyMT-CF cell lines, PyMT-CL parental cells were transduced with a lentiviral luciferase expression vector (Le

Snippet: ted 48 h later. The supernatant was clarified by centrifugation at 350 × g for 5 min and filtered through a 0.45 μm PES syringe filter. Lentivirus was concentrated using Lenti Concentrator (Origene, cat# TR30026). HH514–16 Burkitt lymphoma cells and LCLs were transduced with 100 μl of concentrated virus in the presence of 8 μg/ml Polybrene. Spinoculation was performed at 800 × g

Snippet: The virus containing supernatant was concentrated using Virus Concentration Solution (Origene, TR30026) by centrifugation at 3,500 g. The virus pellet was resuspended in PBS, aliquoted, and stored at −80 °C

Snippet: rnatant was replenished with fresh media. At 72 hpt, the 12 mL culture supernatant was cleared by 500 x g 5 min centrifugation followed with 0.45 µm filtering and concentrated by lenti-concentrator (ORIGENE, Cat. no TR30026) into 1mL media. A day before transduction, 4 x 105 Calu-3 cells were seeded in 12 well plates. On the day of transduction, the culture supernatant of the Calu-3 ce