AAV5 Scrambled shRNA Control Particle

SKU CV900015L

AAV5 control particles, shRNA scramble, expressing GFP and puro, >10^13 GC/mL, 1 x 100 uL

Product Data

• Buffer: PBS with 0.001% Pluronic F68
• Promoter: CMV
• Purification: Iodixanol
• Reporter: GFP
• Serotype: AAV-5
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Cell Line Validation: To validate the quality of our AAV control particles, we have developed the following procedure:
  1. AAV genome titer is determined by qPCR.
  2. AAV purity is determined by Silver staining.
  3. AAV transduction efficiency is analyzed by in vitro transduction in HEK293T.
• Shipping: Dry Ice
• Description: AAV5 control particles, shRNA scramble, expressing GFP and puro, >10^13 GC/mL, 1 x 100 uL