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DGCR2 (NM_001184781) Human Untagged Clone

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SKU
SC328894
DGCR2 (untagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2) transcript variant 4
  $561.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol DGCR2
Synonyms DGS-C; IDD; LAN; SEZ-12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC328894 representing NM_001184781.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGTGCCCAAGGCAGACAGCGGCGCCTTCCTGCTGCTCTTCCTGCTCGTGCTCACTGTCACCGAGCCG
CTGCGGCCAGAGCTGCGGTGCAACCCTGGGCAGTTTGCGTGTCGCAGCGGCACCATCCAGTGCATCCCC
CTCCCCTGGCAGTGTGACGGCTGGGCGACTTGCGAGGATGAGAGCGACGAAGCCAACTGTCCAGAAGTG
ACCGGGGAGGTGCGTCCTCATCATGGGAAGGAGGCTGTGGATCCGCGGCAGGGGCGGGCCAGAGGAGGC
GACCCTTCGCACTTCCACGCGGTGAACGTGGCGCAGCCCGTTCGCTTCAGCAGGAAGTGCCCGACAGGG
TGGCACCACTACGAAGGCACGGCCAGCTGCTACCGGGTCTACCTGAGCGGGGAGAACTACTGGGATGCC
GCGCAGACCTGCCAGCGCCTGAATGGCTCTCTCGCCACCTTCTCCACTGACCAGGAGCTGCGCTTTGTC
CTGGCCCAGGAATGGGACCAGCCCGAGCGGAGCTTTGGTTGGAAGGACCAGCGCAAGTTGTGGGTTGGC
TATCAGTATGTTATCACTGGCCGGAACCGCTCCTTGGAAGGTCGCTGGGAGGTGGCATTCAAAGGCTCT
TCAGAGGTGTTCCTGCCCCCAGACCCCATCTTTGCCTCGGCCATGTCTGAGAACGACAACGTGTTCTGT
GCCCAGCTTCAGTGCTTCCATTTCCCCACCCTGCGGCACCACGACCTCCACAGCTGGCACGCCGAGAGC
TGCTACGAGAAGTCTTCATTTCTGTGTAAAAGAAGTCAAACATGTGTTGACATCAAGGACAACGTGGTG
GATGAAGGGTTCTACTTCACCCCTAAGGGGGACGACCCATGCCTGAGCTGCACCTGCCATGGAGGGGAG
CCTGAGATGTGTGTGGCTGCTCTCTGTGAGAGGCCCCAGGGCTGCCAACAGTACCGCAAGGACCCCAAA
GAGTGCTGCAAGTTCATGTGTCTGGACCCAGATGGCAACAGTCTGTTTGACTCCATGGCCAGCGGGATG
CGCCTGGTCGTCAGCTGCATCTCCTCCTTCCTCATCCTGTCACTGCTGCTCTTCATGGTCCACCGGCTG
CGCCAGCGGCGCCGGGAGCGCATCGAGTCCCTGATTGGAGCAAACTTGCACCACTTCAACCTCGGCCGC
AGGATCCCTGGCTTTGATTACGGCCCAGACGGGTTTGGCACGGGCCTCACGCCGCTGCATCTTTCTGAC
GACGGAGAGGGTGGGACTTTCCATTTCCACGACCCTCCACCTCCCTACACGGCATACAAGTACCCGGAC
ATCGGCCAGCCCGACGACCCTCCGCCGCCCTACGAGGCCTCCATCCACCCGGACAGTGTGTTCTATGAC
CCTGCAGACGATGATGCTTTTGAGCCTGTGGAGGTCAGCCTGCCAGCCCCTGGGGATGGTGGGAGTGAA
GGTGCATTACTCCGGCGCCTGGAGCAGCCTCTGCCCACTGCGGGGGCCTCTCTGGCAGACCTGGAAGAC
TCTGCCGACAGCAGCAGCGCCCTGCTCGTGCCCCCTGACCCTGCCCAGAGCGGGAGCACCCCAGCTGCA
GAGGCACTGCCAGGGGGTGGCCGCCACAGCCGCAGCTCCCTCAATACTGTGGTGTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001184781
Insert Size 1644 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001184781.1
RefSeq Size 4495 bp
RefSeq ORF 1644 bp
Locus ID 9993
Cytogenetics 22q11.21
Protein Families Druggable Genome, Transmembrane
MW 60.6 kDa
Summary Deletions of the 22q11.2 have been associated with a wide range of developmental defects (notably DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome and isolated conotruncal cardiac defects) classified under the acronym CATCH 22. The DGCR2 gene encodes a novel putative adhesion receptor protein, which could play a role in neural crest cells migration, a process which has been proposed to be altered in DiGeorge syndrome. Alternative splicing results in multiple transcript variants.provided by RefSeq, May 2010
Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting isoform (4) lacks a short internal segment, compared to isoform 1.
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Documents
Resources
"NM_001184781" in other vectors (4)
SKU Description Size Price
RC230256 DGCR2 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 4 10 ug
$509.00
RC230256L3 Lenti-ORF clone of DGCR2 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 4 10 ug
$809.00
RC230256L4 Lenti-ORF clone of DGCR2 (mGFP-tagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 4 10 ug
$809.00
RG230256 DGCR2 (tGFP-tagged) - Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 4 10 ug
$709.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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