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CTSA (NM_001167594) Human Untagged Clone

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SKU
SC328790
CTSA (untagged)-Human cathepsin A (CTSA) transcript variant 3
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CTSA
Synonyms GLB2; GSL; NGBE; PPCA; PPGB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC328790 representing NM_001167594.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGACTTCCAGTCCCCGGGCGCCTCCTGGAGAGCAAGGACGCGGGGGAGCAGAGATGATCCGAGCCGCG
CCGCCGCCGCTGTTCCTGCTGCTGCTGCTGCTGCTGCTGCTAGTGTCCTGGGCGTCCCGAGGCGAGGCA
GCCCCCGACCAGGACGAGATCCAGCGCCTCCCCGGGCTGGCCAAGCAGCCGTCTTTCCGCCAGTACTCC
GGCTACCTCAAAGGCTCCGGCTCCAAGCACCTCCACTACTGGTTTGTGGAGTCCCAGAAGGATCCCGAG
AACAGCCCTGTGGTGCTTTGGCTCAATGGGGGTCCCGGCTGCAGCTCACTAGATGGGCTCCTCACAGAG
CATGGCCCCTTCCTGATTGCCAATGTGTTATACCTGGAGTCCCCAGCTGGGGTGGGCTTCTCCTACTCC
GATGACAAGTTTTATGCAACTAATGACACTGAGGTCGCCCAGAGCAATTTTGAGGCCCTTCAAGATTTC
TTCCGCCTCTTTCCGGAGTACAAGAACAACAAACTTTTCCTGACCGGGGAGAGCTATGCTGGCATCTAC
ATCCCCACCCTGGCCGTGCTGGTCATGCAGGATCCCAGCATGAACCTTCAGGGGCTGGCTGTGGGCAAT
GGACTCTCCTCCTATGAGCAGAATGACAACTCCCTGGTCTACTTTGCCTACTACCATGGCCTTCTGGGG
AACAGGCTTTGGTCTTCTCTCCAGACCCACTGCTGCTCTCAAAACAAGTGTAACTTCTATGACAACAAA
GACCTGGAATGCGTGACCAATCTTCAGGAAGTGGCCCGCATCGTGGGCAACTCTGGCCTCAACATCTAC
AATCTCTATGCCCCGTGTGCTGGAGGGGTGCCCAGCCATTTTAGGTATGAGAAGGACACTGTTGTGGTC
CAGGATTTGGGCAACATCTTCACTCGCCTGCCACTCAAGCGGATGTGGCATCAGGCACTGCTGCGCTCA
GGGGATAAAGTGCGCATGGACCCCCCCTGCACCAACACAACAGCTGCTTCCACCTACCTCAACAACCCG
TACGTGCGGAAGGCCCTCAACATCCCGGAGCAGCTGCCACAATGGGACATGTGCAACTTTCTGGTAAAC
TTACAGTACCGCCGTCTCTACCGAAGCATGAACTCCCAGTATCTGAAGCTGCTTAGCTCACAGAAATAC
CAGATCCTATTATATAATGGAGATGTAGACATGGCCTGCAATTTCATGGGGGATGAGTGGTTTGTGGAT
TCCCTCAACCAGAAGATGGAGGTGCAGCGCCGGCCCTGGTTAGTGAAGTACGGGGACAGCGGGGAGCAG
ATTGCCGGCTTCGTGAAGGAGTTCTCCCACATCGCCTTTCTCACGATCAAGGGCGCCGGCCACATGGTT
CCCACCGACAAGCCCCTCGCTGCCTTCACCATGTTCTCCCGCTTCCTGAACAAGCAGCCATACTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001167594
Insert Size 1446 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001167594.2
RefSeq Size 3011 bp
RefSeq ORF 1446 bp
Locus ID 5476
UniProt ID P10619
Cytogenetics 20q13.12
Protein Families Druggable Genome, Protease
Protein Pathways Renin-angiotensin system
MW 54.3 kDa
Summary This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. provided by RefSeq, Nov 2015
Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The encoded isoform (c) is shorter than isoform a. This isoform may undergo proteolytic processing similar to isoform b.
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Documents
Resources
"NM_001167594" in other vectors (4)
SKU Description Size Price
RC230152 CTSA (Myc-DDK-tagged)-Human cathepsin A (CTSA), transcript variant 3 10 ug
$457.00
RC230152L3 Lenti-ORF clone of CTSA (Myc-DDK-tagged)-Human cathepsin A (CTSA), transcript variant 3 10 ug
$757.00
RC230152L4 Lenti-ORF clone of CTSA (mGFP-tagged)-Human cathepsin A (CTSA), transcript variant 3 10 ug
$757.00
RG230152 CTSA (tGFP-tagged) - Human cathepsin A (CTSA), transcript variant 3 10 ug
$657.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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