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GFAP (NM_001131019) Human Untagged Clone

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SKU
SC325916
GFAP (untagged)-Human glial fibrillary acidic protein (GFAP), transcript variant 2
  $503.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol GFAP
Synonyms ALXDRD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI |
ACCN NM_001131019
Insert Size 1296 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001131019.2
RefSeq Size 1839 bp
RefSeq ORF 1296 bp
Locus ID 2670
UniProt ID P14136
Cytogenetics 17q21.31
Protein Families ES Cell Differentiation/IPS
MW 49.5 kDa
Summary This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. provided by RefSeq, Oct 2008
Transcript Variant: This variant (2) differs at the 3' coding region and 3' UTR, compared to variant 1, which results in a protein (isoform 2, also known as isoform epsilon) with a shorter and distinct C-terminus when compared to isoform 1.
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Documents
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"NM_001131019" in other vectors (4)
SKU Description Size Price
RC225707 GFAP (Myc-DDK-tagged)-Human glial fibrillary acidic protein (GFAP), transcript variant 2 10 ug
$457.00
RC225707L3 Lenti ORF clone of Human glial fibrillary acidic protein (GFAP), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC225707L4 Lenti ORF clone of Human glial fibrillary acidic protein (GFAP), transcript variant 2, mGFP tagged 10 ug
$757.00
RG225707 GFAP (tGFP-tagged) - Human glial fibrillary acidic protein (GFAP), transcript variant 2 10 ug
$489.00 $657.00

file-alt Citations

Clinical and immunological characteristics of the spectrum of GFAP autoimmunity: a case series of 22 patients
Iorio, Damato, Evoli et al
J. Neurol. Neurosurg. Psychiatr. (2017)
AI Summary: OriGene products pCMV6-XL6-GFAPα (SC118873) and pCMV6-Entry-GFAPδ (SC325916) were used to transfect HEK-293 cells for cell-based assays, enabling the detection and confirmation of GFAP autoantibodies in patient samples in this study.
Snippet: … HEK-293 cells were transfected with plasmids encoding GFAP homo sapiens transcript variant 1 (SC118873, pCMV6-XL6-GFAPα) and variant 2 (SC325916, pCMV6-Entry-GFAPδ, OriGene) using Lipofectamine 3000 (Invitrogen). ...;... … HEK-293 cells were transfected with plasmids encoding GFAP homo sapiens transcript variant 1 (SC118873, pCMV6-XL6-GFAPα) and variant 2 (SC325916, pCMV6-Entry-GFAPδ, OriGene) using Lipofectamine 3000 (Invitrogen). ...;... … HEK-293 cells were transfected with plasmids encoding GFAP homo sapiens transcript variant 1 (SC118873, pCMV6-XL6-GFAPα) and variant 2 (SC325916, pCMV6-Entry-GFAPδ, OriGene) using Lipofectamine 3000 (Invitrogen).
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