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ECHDC1 (NM_001139510) Human Untagged Clone

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SKU
SC325747
ECHDC1 (untagged)-Human enoyl CoA hydratase domain containing 1 (ECHDC1), transcript variant 5
  $330.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol ECHDC1
Synonyms dJ351K20.2; HEL-S-76; MMCD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC325747 representing NM_001139510.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCATTGAAACAGGAAATGGCGAAAAGTCTTTTGAAGACAGCCTCTCTGTCTGGAAGGACAAAATTG
CTACATCAAACAGGATTGTCACTTTATAGTACATCCCATGGATTTTATGAGGAAGAAGTGAAAAAAACA
CTTCAGCAGTTTCCTGGTGGATCCATTGACCTTCAGAAGGAAGACAATGGCATTGGCATTCTTACTCTG
AACAATCCAAGTAGAATGAATGCCTTTTCAGGTGTTATGATGCTACAACTTCTGGAAAAAGTAATTGAA
TTGGAAAATTGGACAGAGGGGAAAGGCCTCATTGTCCGTGGGGCAAAAAATACTTTCTCTTCAGGATCT
GATCTGAATGCTGTGAAATCACTAGGAACTCCAGAGGATGGAATGGCCGTATGCATGTTCATGCAAAAC
ACCTTAACAAGATTTATGAGACTTCCTTTAATAAGTGTTGCGCTGGTTCAAGGTTGGGCATTGGGTGGA
GGAGCAGAATTTACTACAGCATGTGATTTCAGGTTAATGACTCCAGAGAGTAAGATCAGATTCGTCCAC
AAAGAGATGGGCATAATACCAAGCTGGGGTGGCACCACCCGGCTAGTTGAAATAATCGGAAGTAGACAA
GCTCTCAAAGTGTTGAGTGGGGCCCTTAAACTGGATTCAAAAAATGCTCTAAACATAGGAATGGTTGAA
GAGGTCTTGCAGTCTTCAGATGAAACTAAATCTCTAGAAGAGGCACAAGAATGGCTAAAGCAATTCATC
CAAGGGCCACCGGAAGTAATTAGAGCTTTGAAAAAATCTGTTTGTTCAGGCAGAGAGCTATATTTGGAG
GAAGCATTACAGAACGAAAGAGATCTTTTAGGAACAGTTTGGGGTGGGCCTGCAAATTTAGAGGCTATT
GCTAAGAAAGGAAAATTTAATAAATAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001139510
Insert Size 924 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001139510.1
RefSeq Size 2131 bp
RefSeq ORF 924 bp
Locus ID 55862
UniProt ID Q9NTX5
Cytogenetics 6q22.33
MW 33.7 kDa
Summary Decarboxylases ethylmalonyl-CoA decarboxylase, a potentially toxic metabolite, to form butyryl-CoA, suggesting it might be involved in metabolite proofreading. Also has methylmalonyl-CoA decarboxylase activity at lower level.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (5) uses an alternate 5' exon, thus differing in its 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (5) is longer at the N-terminus, compared to isoform 1.
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Documents
Resources
"NM_001139510" in other vectors (4)
SKU Description Size Price
RC226894 ECHDC1 (Myc-DDK-tagged)-Human enoyl CoA hydratase domain containing 1 (ECHDC1), transcript variant 5 10 ug
$300.00
RC226894L3 Lenti ORF clone of Human enoyl CoA hydratase domain containing 1 (ECHDC1), transcript variant 5, Myc-DDK-tagged 10 ug
$600.00
RC226894L4 Lenti ORF clone of Human enoyl CoA hydratase domain containing 1 (ECHDC1), transcript variant 5, mGFP tagged 10 ug
$600.00
RG226894 ECHDC1 (tGFP-tagged) - Human enoyl CoA hydratase domain containing 1 (ECHDC1), transcript variant 5 10 ug
$500.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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