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SAP30L (NM_001131063) Human Untagged Clone

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SKU
SC325504
SAP30L (untagged)-Human SAP30-like (SAP30L), transcript variant 3
  $165.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SAP30L
Synonyms NS4ATP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC325504 representing NM_001131063.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAACGGCTTCAGCACGGAGGAGGACAGCCGCGAAGGGCCCCCCGCCGCCCCAGCTGCCGCCGCCCCG
GGCTACGGCCAGAGCTGCTGCCTCATCGAGGACGGCGAGCGCTGCGTCCGGCCCGCGGGCAACGCCTCC
TTCAGCAAGAGGGTCCAGAAGAGCATCTCGCAGAAGAAACTCAAGCTGGACATCGACAAGAGCCTGCAG
GTGAACACCCTACGACGTTATAAACGACACTACAAGTTGCAGACCAGACCAGGCTTCAATAAGGCCCAG
TTAGCAGAAACTGTGAGTCGACACTTCAGGAACATACCTGTGAATGAAAAAGAGACCCTTGCCTACTTC
ATCTACATGGTGAAGAGTAACAAGAGTAGACTGGACCAGAAATCGGAGGGTGGCAAGCAGCTTGAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001131063
Insert Size 414 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001131063.1
RefSeq Size 6105 bp
RefSeq ORF 414 bp
Locus ID 79685
UniProt ID Q9HAJ7
Cytogenetics 5q33.2
MW 15.4 kDa
Summary Isoform 1: Functions as transcription repressor, probably via its interaction with histone deacetylase complexes (PubMed:16820529, PubMed:18070604). Involved in the functional recruitment of the class 1 Sin3-histone deacetylase complex (HDAC) to the nucleolus (PubMed:16820529). Binds DNA, apparently without sequence-specificity, and bends bound double-stranded DNA (PubMed:19015240). Binds phosphoinositol phosphates (phosphoinositol 3-phosphate, phosphoinositol 4-phosphate and phosphoinositol 5-phosphate) via the same basic sequence motif that mediates DNA binding and nuclear import (PubMed:19015240, PubMed:26609676).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) lacks an alternate in-frame exon and uses an alternate in-frame splice site, compared to variant 1. The resulting protein (isoform 3) is shorter than isoform 1.
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Documents
Resources
"NM_001131063" in other vectors (4)
SKU Description Size Price
RC225107 SAP30L (Myc-DDK-tagged)-Human SAP30-like (SAP30L), transcript variant 3 10 ug
$150.00
RC225107L3 Lenti ORF clone of Human SAP30-like (SAP30L), transcript variant 3, Myc-DDK-tagged 10 ug
$450.00
RC225107L4 Lenti ORF clone of Human SAP30-like (SAP30L), transcript variant 3, mGFP tagged 10 ug
$450.00
RG225107 SAP30L (tGFP-tagged) - Human SAP30-like (SAP30L), transcript variant 3 10 ug
$350.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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