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CASKIN2 (NM_001142643) Human Untagged Clone

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SKU
SC325393
CASKIN2 (untagged)-Human CASK interacting protein 2 (CASKIN2), transcript variant 2
  $1,073.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CASKIN2
Synonyms ANKS5B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC325393 representing NM_001142643.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCGCCCGCTGCACTACGCAGCCTGGCAGGGCCGGCTGGAGCCTGTGAGGCTGCTGCTGCGCGCCTCT
GCGGCTGTCAATGCCGCCTCGCTGGACGGACAGATCCCCCTGCACCTGGCTGCACAGTATGGACATTAT
GAGGTGTCAGAAATGCTCCTCCAGCATCAGTCCAACCCATGCCTGGTCAACAAGGCCAAGAAGACGCCC
CTGGACCTGGCCTGTGAATTTGGCCGACTCAAGGTGGCCCAGCTGCTTCTGAACAGCCACTTATGTGTG
GCACTGCTGGAGGGTGAGGCCAAAGACCCGTGTGACCCCAACTACACCACGCCCCTGCACTTGGCTGCC
AAGAATGGCCACAGAGAAGTCATCAGGCAGCTCCTGAGAGCTGGGATCGAGATCAACCGCCAGACCAAG
ACGGGTACGGCGCTCCACGAGGCCGCACTGTATGGCAAGACCGAGGTGGTGCGGCTGCTTCTGGAGGGA
GGTGTGGACGTGAACATCCGGAATACGTATAACCAGACGGCGCTGGACATAGTGAATCAGTTCACCACC
TCCCAGGCCAGCCGGGAAATCAAGCAGCTACTGCGGGAGGCCTCAGGGATCCTGAAGGTCCGAGCGCTC
AAGGATTTCTGGAACCTCCACGATCCCACTGCTCTCAATGTCCGGGCAGGGGATGTCATCACGGTGCTA
GAACAGCATCCCGACGGCCGCTGGAAGGGCCACATCCACGAGAGCCAGAGGGGCACAGACCGCATAGGC
TACTTCCCCCCGGGCATTGTCGAGGTGGTCAGCAAGCGGGTGGGCATCCCTGCAGCCCGCCTCCCCTCC
GCACCCACCCCCCTGCGCCCAGGCTTCTCCCGGACACCGCAGCCTCCTGCCGAAGAACCCCCGCACCCT
CTTACCTACAGCCAGCTTCCTCGGGTGGGCCTCAGCCCAGACAGCCCAGCAGGTGACAGGAATAGTGTG
GGCAGTGAGGGCAGCGTGGGCAGCATCCGCAGTGCCGGCAGCGGGCAGAGCTCTGAGGGCACTAATGGC
CATGGCCCTGGCCTCCTGATTGAGAACGCCCAGCCACTGCCCTCTGCTGGAGAGGACCAGGTGCTGCCA
GGACTCCACCCGCCGTCCCTGGCAGACAACCTGAGCCACCGCCCTCTGGCCAACTGCCGCTCTGGGGAG
CAGATCTTCACCCAGGACGTGCGGCCAGAACAGCTGCTGGAGGGGAAGGACGCGCAGGCCATTCATAAC
TGGCTAAGCGAGTTCCAGCTGGAGGGCTACACTGCCCACTTTCTGCAGGCCGGCTATGATGTGCCTACC
ATCAGCCGCATGACACCTGAGGACCTGACGGCCATCGGGGTGACCAAGCCTGGGCACAGGAAGAAGATC
GCCTCAGAGATCGCTCAGCTCAGCATCGCCGAGTGGCTGCCCAGCTACATCCCAACGGACCTGCTGGAG
TGGCTGTGTGCACTGGGGCTGCCACAGTACCACAAGCAGCTGGTGAGCAGCGGCTACGACTCCATGGGG
CTGGTGGCCGACCTCACCTGGGAGGAGCTGCAGGAGATTGGGGTCAACAAGCTCGGGCATCAGAAGAAG
CTCATGCTGGGGGTGAAGCGGCTGGCGGAGCTTCGGCGGGGCCTGCTGCAGGGGGAGGCCCTCAGCGAA
GGCGGGCGCCGGCTGGCCAAGGGTCCGGAGCTGATGGCCATCGAGGGACTGGAGAACGGAGAAGGCCCA
GCTACAGCTGGCCCACGGCTCCTCACCTTCCAGGGCAGCGAACTAAGCCCAGAGCTACAGGCGGCCATG
GCAGGGGGTGGCCCTGAACCACTCCCCCTCCCACCTGCCCGCTCTCCCAGCCAGGAGAGCATCGGGGCA
CGCTCACGGGGGTCTGGCCACTCACAGGAACAGCCTGCCCCACAGCCCAGCGGTGGAGATCCCAGCCCC
CCCCAGGAGAGGAACCTCCCAGAGGGCACAGAGCGGCCCCCTAAGCTTTGTTCTTCACTTCCTGGCCAA
GGACCCCCACCCTATGTTTTTATGTACCCCCAGGGCTCACCCTCTAGCCCGGCCCCAGGGCCACCTCCT
GGCGCACCCTGGGCCTTCTCCTACTTGGCCGGGCCCCCTGCCACTCCCCCAGACCCGCCTCGACCTAAG
CGCCGGTCCCACAGCCTAAGCCGCCCTGGCCCCACAGAGGGGGATGCTGAGGGGGAGGCCGAAGGGCCA
GTGGGCAGCACCCTAGGCAGTTATGCTACCCTTACCCGGCGGCCAGGACGCAGTGCCCTTGTCCGGACC
AGTCCTAGTGTGACCCCAACCCCAGCTCGGGGGACTCCTCGCAGCCAGTCCTTTGCCCTGCGGGCCCGG
CGCAAAGGCCCCCCGCCCCCGCCCCCCAAGCGCCTCAGCTCCGTCTCTGGCCCCAGCCCGGAGCCACCT
CCACTAGATGAGAGCCCAGGGCCCAAGGAAGGGGCCACAGGGCCCCGAAGGCGAACACTGAGTGAACCT
GCTGGCCCCTCAGAGCCCCCTGGCCCACCTGCCCCGGCTGGGCCCGCGTCAGACACGGAGGAGGAGGAG
CCAGGCCCTGAGGGGACGCCCCCATCTCGGGGCAGCTCTGGGGAAGGGCTGCCGTTTGCAGAGGAAGGG
AACCTGACCATCAAACAGCGCCCGAAGCCCGCTGGCCCCCCGCCCCGAGAGACACCCGTGCCCCCCGGC
CTCGATTTCAACCTCACGGAATCAGACACTGTTAAGCGGAGGCCCAAGTGCCGGGAGAGAGAGCCACTG
CAGACCGCACTGCTGGCCTTCGGAGTGGCCAGTGCCACGCCTGGCCCCGCTGCCCCACTGCCTTCCCCA
ACTCCTGGCGAGTCTCCTCCAGCTTCTAGCCTTCCCCAGCCCGAGCCCAGCAGCCTTCCAGCCCAAGGA
GTTCCAACCCCCCTTGCTCCCAGCCCCGCCATGCAGCCTCCAGTGCCGCCCTGCCCAGGGCCAGGTCTG
GAAAGCTCAGCAGCTAGTCGGTGGAATGGGGAGACAGAACCCCCGGCCGCCCCTGCTGCCCTCCTCAAG
GTGCCCGGAGCAGGAACAGCCCCCAAGCCTGTGTCGGTGGCCTGCACCCAGCTGGCATTTTCTGGCCCT
AAGCTAGCGCCCCGGCTCGGCCCCCGCCCAGTGCCTCCTCCACGGCCTGAGAGCACTGGGACTGTGGGC
CCAGGCCAGGCCCAGCAGAGACTGGAGCAGACCAGCTCGTCCCTGGCAGCTGCACTGAGAGCCGCAGAG
AAGAGCATTGGCACCAAGGAGCAAGAGGGCACCCCCAGCGCCTCCACCAAGCACATTCTGGATGACATC
AGCACCATGTTCGACGCCCTGGCTGACCAGCTGGACGCCATGCTGGACTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001142643
Insert Size 3363 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001142643.2
RefSeq Size 4515 bp
RefSeq ORF 3363 bp
Locus ID 57513
UniProt ID Q8WXE0
Cytogenetics 17q25.1
MW 118.2 kDa
Summary This gene encodes a large protein that contains six ankyrin repeats, as well as a Src homology 3 (SH3) domain and two sterile alpha motif (SAM) domains, which may be involved in protein-protein interactions. The C-terminal portion of this protein is proline-rich and contains a conserved region. A related protein interacts with calcium/calmodulin-dependent serine protein kinase (CASK). Alternative splicing results in multiple transcript variants. provided by RefSeq, Mar 2013
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.
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Documents
Resources
"NM_001142643" in other vectors (4)
SKU Description Size Price
RC227932 CASKIN2 (Myc-DDK-tagged)-Human CASK interacting protein 2 (CASKIN2), transcript variant 2 10 ug
$975.00
RC227932L3 Lenti-ORF clone of CASKIN2 (Myc-DDK-tagged)-Human CASK interacting protein 2 (CASKIN2), transcript variant 2 10 ug
$1,275.00
RC227932L4 Lenti-ORF clone of CASKIN2 (mGFP-tagged)-Human CASK interacting protein 2 (CASKIN2), transcript variant 2 10 ug
$1,275.00
RG227932 CASKIN2 (tGFP-tagged) - Human CASK interacting protein 2 (CASKIN2), transcript variant 2 10 ug
$1,175.00

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