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NFIB (NM_005596) Human Untagged Clone

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SKU
SC323795
NFIB (untagged)-Human nuclear factor I/B (NFIB), transcript variant 3
  $457.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NFIB
Synonyms CTF; HMGIC/NFIB; MACID; NF-I/B; NF1-B; NFI-B; NFI-RED; NFIB2; NFIB3
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_005596.2 GACTGGGTTTGTTGTGAAGTTGCGGTGATCCAGCGCAGAGCCCCGTCCTGATTGATCGCA
TCGCGGGGCTCAGATGACTGTAAAATGAATAGATGAAATTCTTGCTTCTCGAAGATTTTC
TTGGGCATCTCCCGGAAAGTGCGTTTTAAGGCGAAGTCATGATGTATTCTCCCATCTGTC
TCACTCAGGATGAATTTCACCCATTCATCGAGGCACTTCTTCCACATGTCCGTGCAATTG
CCTATACTTGGTTCAACCTGCAGGCTCGAAAACGCAAGTACTTTAAAAAGCATGAGAAGC
GAATGTCAAAGGATGAAGAAAGAGCAGTCAAAGATGAGCTTCTCAGTGAAAAGCCTGAAA
TCAAACAGAAGTGGGCATCCAGGCTCCTTGCCAAACTGCGCAAAGATATTCGCCAGGAGT
ATCGAGAGGACTTTGTGCTCACCGTGACTGGCAAGAAGCACCCGTGCTGTGTCTTATCCA
ATCCCGACCAGAAGGGTAAGATTAGGAGAATCGACTGCCTGCGACAGGCAGACAAAGTCT
GGCGTCTGGATCTAGTCATGGTGATCCTGTTCAAAGGCATCCCCTTGGAAAGTACCGATG
GAGAGCGGCTCATGAAATCCCCACATTGCACAAACCCAGCACTTTGTGTCCAGCCACATC
ATATCACAGTATCAGTTAAGGAGCTTGATTTGTTTTTGGCATACTACGTGCAGGAGCAAG
ATTCTGGACAATCAGGAAGTCCAAGCCACAATGATCCTGCCAAGAATCCTCCAGGTTACC
TTGAGGATAGTTTTGTAAAATCTGGAGTCTTCAATGTATCAGAACTTGTAAGAGTATCCA
GAACGCCCATAACCCAGGGAACTGGAGTCAACTTCCCAATTGGAGAAATCCCAAGCCAAC
CATACTATCATGACATGAACTCGGGGGTCAATCTTCAGAGGTCTCTGTCTTCTCCACCAA
GCAGCAAAAGACCCAAAACTATATCCATAGATGAAAATATGGAACCAAGTCCTACAGGAG
ACTTTTACCCCTCTCCAAGTTCACCAGCTGCTGGAAGTCGAACATGGCACGAAAGAGATC
AAGATATGTCTTCTCCGACTACTATGAAGAAGCCTGAAAAGCCATTGTTCAGCTCTGCAT
CTCCACAGGATTCTTCCCCAAGACTGAGCACTTTCCCCCAGCACCACCATCCCGGAATAC
CTGGAGTTGCACACAGTGTCATCTCAACTCGAACTCCACCTCCACCTTCACCGTTGCCAT
TTCCAACACAAGCTATCCTTCCTCCAGCCCCATCGAGCTACTTTTCTCATCCAACAATCA
GATATCCTCCCCACCTGAATCCTCAGGATACTCTGAAGAACTATGTACCTTCTTATGACC
CATCCAGTCCACAAACCAGCCAGTCCTGGTACCTGGGCTAGCTTGGTTCCTTTCCAAGTG
TCAAATAGGACACCCATCTTACCGGCCAATGTCCAAAATTACGGTTTGAACATAATTGGA
GAACCTTTCCTTCAAGCAGAAACAAGCAACTGAGGGAAAAAGAAACACAACAATAGTTTA
AGAAATTTTTTTTTTAAATAAAAAAAAAGGAAAAGAGGAAGACTGGACAAAACAACACAA
AGGCAGAAAGGAAAGAAACTGAAGAAAGAAGATAATAGACCAGCAATTGCAGCACTTACA
ATCACTAATTCCCTTAAGGTTGAAACTGTAATGACATAAAAAGGGTCGATGATATTTCAC
TGATGGTAGATCGCAGCCCCTGCAACGTAGCCTTTGTTACATGAAGTCCGCTGGGAAATA
GATGTTCTGTCTCTATGACAATATATTTTAACTGACTTTCTAGATGCCTTAATATTTGCA
TGATAAGCTAGTTTTATTGGTTTAGTATTCTTGTTGTTTACGCATGGAATCACTATTCCT
GGTTATCTCACCAACGAAGGCTAGGAGGCGGCGTCAGAGGTGCTGGGTGACAGAGCCATG
AGCCAGCCATTTTATAAGCACTCTGATTTCTAAAAGTTAAAAAAAATATATGAAATCTCT
GTAGCCTTTAGTTATCAGTACAGATTTATTAAATTTCGGCCCTTAACCCAGCCTTTTCCA
GTGTGTAACCCAGTTTGAAATCTTAAAAAAAGAAAAAAAAAAAAAAAA
Restriction Sites ECoRI-NOT |
ACCN NM_005596
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005596.2, NP_005587.2
RefSeq Size 8159 bp
RefSeq ORF 1263 bp
Locus ID 4781
UniProt ID O00712
Cytogenetics 9p23-p22.3
Domains CTF_NFI, DWA
Protein Families Druggable Genome, Transcription Factors
Summary Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) lacks alternate exons in the 3' end, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_005596" in other vectors (7)
SKU Description Size Price
RC201590 NFIB (Myc-DDK-tagged)-Human nuclear factor I/B (NFIB), transcript variant 3 10 ug
$457.00
RC201590L1 Lenti ORF clone of Human nuclear factor I/B (NFIB), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC201590L2 Lenti ORF clone of Human nuclear factor I/B (NFIB), transcript variant 3, mGFP tagged 10 ug
$757.00
RC201590L3 Lenti ORF clone of Human nuclear factor I/B (NFIB), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC201590L4 Lenti ORF clone of Human nuclear factor I/B (NFIB), transcript variant 3, mGFP tagged 10 ug
$757.00
RG201590 NFIB (tGFP-tagged) - Human nuclear factor I/B (NFIB), transcript variant 3 10 ug
$489.00 $657.00
SC116683 NFIB (untagged)-Human nuclear factor I/B (NFIB), transcript variant 3 10 ug
$457.00

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