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PRPF4B (NM_003913) Human Untagged Clone

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SKU
SC323624
PRPF4B (untagged)-Kinase deficient mutant (K717M) of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B)
  $877.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol PRPF4B
Synonyms dJ1013A10.1; PR4H; PRP4; PRP4H; PRP4K
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF within SC323624 sequence for NM_003913 edited (data generated by NextGen Sequencing)
ATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAGAGATGGAAGATGCTAATTCT
GAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCAAAATAAGCAC
AGTCGTCACAAAAAAAAGAAGCATAAACACAGAAGTAAACATAAGAAACATAAACATTCC
TCAGAAGAAGACAAGGATAAAAAACATAAACATAAGCATAAACATAAGAAACACAAAAGA
AAAGAGATTATTGATGCTTCTGATAAAGAGGGTATGTCTCCAGCAAAAAGAACTAAACTT
GATGATTTAGCTTTGCTAGAAGACTTGGAAAAACAGAGAGCCTTGATTAAGGCCGAACTT
GATAATGAGTTAATGGAAGGAAAGGTCCAGTCTGGTATGGGGCTCATTTTGCAAGGTTAT
GAGTCTGGCTCTGAAGAAGAGGGGGAAATTCATGAAAAGGCAAGAAATGGAAATAGGTCT
AGTACTAGATCTTCAAGTACAAAGGGGAAACTTGAACTTGTGGACAATAAAATTACTACA
AAGAAACGAAGTAAAAGCAGATCCAAAGAACGGACTAGACATAGGTCTGATAAAAAGAAA
AGTAAGGGGGGTATTGAAATCGTTAAAGAGAAAACAACTAGGAGCAAGTCAAAGGAGAGG
AAAAAATCTAAAAGCCCATCCAAAAGAAGTAAGTCTCAAGATCAAGCAAGGAAATCAAAA
TCCCCTACCCTTAGAAGGCGATCTCAAGAGAAAATTGGTAAGGCCAGATCTCCTACTGAT
GATAAGGTTAAAATTGAAGATAAAAGTAAATCAAAAGATAGGAAAAAATCCCCAATTATA
AATGAAAGTAGAAGTCGCGATCGAGGTAAAAAATCCAGATCCCCAGTTGATTTAAGAGGT
AAATCCAAAGACAGAAGGTCACGGTCCAAAGAGAGAAAATCAAAACGGTCTGAAACTGAT
AAAGAAAAGAAGCCAATTAAATCTCCCTCTAAAGATGCTTCATCTGGGAAAGAAAATAGG
TCACCCAGCAGAAGACCTGGTCGTAGTCCTAAAAGAAGAAGTTTGTCTCCAAAACCACGT
GATAAATCAAGAAGAAGCAGGTCTCCACTTTTGAATGATAGAAGATCTAAGCAGAGCAAA
TCCCCCTCGCGGACACTGTCTCCTGGGAGAAGAGCCAAGAGCCGATCCTTAGAAAGAAAA
CGACGAGAACCAGAGAGGAGACGACTTTCTTCTCCAAGAACACGACCTCGAGATGATATC
CTCAGTAGACGTGAAAGATCAAAAGATGCCAGCCCCATCAATAGATGGTCTCCAACCCGA
AGAAGAAGTAGATCTCCCATTAGAAGGAGGTCTCGTTCCCCACTCAGACGTAGCAGGTCT
CCAAGAAGAAGAAGCAGATCTCCTCGGAGAAGGGACAGAGGTCGGAGGAGCAGATCACGC
TTGCGAAGGCGGTCTCGATCACGCGGTGGTCGTAGACGAAGGAGCAGAAGCAAAGTAAAG
GAAGATAAATTTAAAGGAAGTCTTTCTGAAGGAATGAAAGTTGAGCAGGAATCTTCGTCT
GATGATAACCTTGAAGACTTTGATGTAGAGGAAGAAGATGAAGAAGCCCTAATAGAACAG
AGAAGAATCCAAAGGCAGGCAATTGTTCAGAAATATAAATACCTTGCTGAAGATAGCAAC
ATGTCTGTGCCATCTGAACCAAGCAGCCCCCAGAGCAGTACGAGAACACGATCACCATCT
CCAGATGACATTCTGGAGCGAGTAGCTGCTGATGTTAAAGAGTATGAACGGGAAAATGTT
GATACATTTGAGGCCTCAGTGAAAGCCAAGCATAATCTAATGACAGTTGAACAGAATAAT
GGTTCATCTCAGAAGAAGTTGTTGGCACCTGATATGTTTACAGAATCTGATGATATGTTT
GCTGCGTATTTTGATAGTGCTCGTCTTCGGGCCGCTGGCATTGGAAAAGATTTCAAAGAG
AATCCCAACCTCAGAGATAACTGGACCGATGCAGAAGGCTATTATCGTGTGAACATAGGT
GAAGTCCTAGATAAACGTTACAATGTGTATGGCTACACTGGGCAAGGTGTATTCAGTAAT
GTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAAGAAGTGGCTGTAATGATCATCAGA
AACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCTTGAAAAAACTTAAT
GATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTCTTCAGGCACTTCTATCACAAG
CAGCATCTTTGTCTGGTATTCGAGCCTCTCAGCATGAACTTACGAGAGGTGTTAAAAAAA
TATGGTAAAGATGTTGGTCTTCATATTAAAGCTGTAAGATCCTATAGTCAGCAGTTGTTC
CTGGCATTGAAACTCCTTAAAAGATGCAATATCCTACATGCAGATATCAAGCCAGACAAT
ATCCTGGTTAATGAATCCAAAACTATTTTAAAGCTTTGCGATTTTGGGTCGGCTTCACAT
GTTGCGGATAATGACATAACACCTTATCTTGTCAGTAGATTTTATCGTGCTCCTGAAATC
ATTATAGGTAAAAGCTATGACTATGGTATAGATATGTGGTCTGTAGGTTGCACCTTATAC
GAACTCTATACTGGAAAAATTTTATTCCCTGGCAAAACCAATAACCATATGCTGAAGCTT
GCAATGGATCTCAAAGGAAAGATGCCAAATAAGATGATTCGAAAAGGTGTGTTCAAAGAT
CAGCATTTTGATCAAAATCTCAACTTCATGTACATAGAAGTTGATAAAGTAACAGAGAGG
GAGAAAGTTACTGTTATGAGCACCATTAATCCAACTAAGGACCTGTTGGCTGACTTGATT
GGGTGCCAGAGACTTCCTGAAGACCAACGTAAGAAAGTACACCAGCTAAAGGACTTGTTG
GACCAGATTCTGATGTTGGACCCAGCTAAACGAATTAGCATCAACCAGGCCCTACAGCAC
GCCTTCATCCAGGAAAAAATTTAA

Clone variation with respect to NM_003913.4
2150 a=>t
5' Read Nucleotide Sequence
>OriGene 5' read for mutant NM_003913 unedited
CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGAGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGGAGCCGCCGC
CACCGCCGCCGAGGAGTCAGGAAGTTCAAGATGGCCGCCGCGGAGACCCAGTCGCTACGGGAGCAGCCAG
AGATGGAAGATGCTAATTCTGAAAAGAGTATAAATGAAGAAAATGGAGAAGTATCAGAAGACCAGTCTCA
AAATAAGCACAGTCGTCACAAAAAAAAGAAGCATAAACACAGAAGTAAACATAAGAAACATAAACGTTCC
TCAGAGGAGAACAAGGATAAAAAACATAAACATAAGCTTAACCTTAAGAAACCCAAAAGAAAAGGAGATT
ATTGATGGCTTCCTGATAAGAAGGGTATGGTCTCCGGCAAAAAGACCTAAACTGATTGATTTACCTTGGT
TGGAGAACTTGGAAAAACGGAGAGCCTGGTTAAAGGCGAACTTGTTAGGAGTTTGGGAGGGAGGGTCCGG
TTGTGATGGGGGCTATTTTGCAGGTTAGACCGGTTCTGAAGAGGGGGGAATTCGGAAAGGGAAAATGAAA
TGGTATATATATATCTCGATAAAGGGGACTCTGCTTTGTGCCCTAAAATTCCTCAGAACGAATTAACCGT
CCCAGACGGCTTCCTTGGTGGATAAGAAGATGGGGGTTGAACGTTAGGAAACTTGGCGTCTAGGAGAAAT
TAGCTCCAAATGTCCATAGCGGACATCTCTCTAGAGTCAGAATGAGCGATCTCGAAAGGATGAAGATACA
TAGGATCCTAAGTAGTGCTTCGTAATCTTCTTTG
Kinase Domain Sequence
>SC323624 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GKGCWTGGCTAMCTGGGCAGGTGTATTCAGTAATGTTGTACGAGCCAGAGATAATGCAAGAGCCAACCAA
GAAGTGGCTGTAATGATCATCAGAAACAATGAGCTCATGCAAAAGACTGGTTTAAAAGAATTAGAGTTCT
TGAAAAAACTTAATGATGCTGATCCTGATGACAAATTTCATTGTCTGAGACTCTTCAGGCACTTCTATCA
CAAGCAGCATCTTTGTCTGGTATTCGAGCCTCTCAGCATGAACTT
Restriction Sites Please inquire |
ACCN NM_003913
Insert Size 5000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003913.3, NP_003904.2
RefSeq Size 6681 bp
RefSeq ORF 3024 bp
Locus ID 8899
UniProt ID Q13523
Cytogenetics 6p25.2
Domains pkinase, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
Summary Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). provided by RefSeq, Jul 2008
Transcript Variant: This variant (1) represents the protein-coding transcript.
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Documents
Resources
"NM_003913" in other vectors (7)
SKU Description Size Price
RC205508 PRPF4B (Myc-DDK-tagged)-Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) 10 ug
$877.00
RC205508L1 Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), Myc-DDK-tagged 10 ug
$1,177.00
RC205508L2 Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), mGFP tagged 10 ug
$1,177.00
RC205508L3 Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), Myc-DDK-tagged 10 ug
$1,177.00
RC205508L4 Lenti ORF clone of Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B), mGFP tagged 10 ug
$1,177.00
RG205508 PRPF4B (tGFP-tagged) - Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) 10 ug
$789.00 $1,077.00
SC107962 PRPF4B (untagged)-Human PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) 10 ug
$877.00

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