TAOK2 (NM_004783) Human Untagged Clone

SKU SC323586

TAOK2 (untagged)-Kinase deficient mutant (K57M) of Human TAO kinase 2 (TAOK2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: TAOK2
• Synonyms: MAP3K17; PSK; PSK1; PSK1-BETA; TAO1; TAO2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >SC323586 representing NM_004783.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; ATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGGACCCAGATGTGGCTGAGCTCTTCTTCAAGGATGAC; CCAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGGCCATGGCAGCTTTGGAGCCGTATACTTTGCCCGG; GATGTCCGGAATAGTGAGGTGGTGGCCATCATGAAGATGTCCTACAGTGGGAAGCAGTCCAATGAGAAA; TGGCAAGACATCATCAAGGAGGTGCGGTTCTTACAGAAGCTCCGGCATCCCAACACCATTCAGTACCGG; GGCTGTTACCTGAGGGAGCACACGGCTTGGCTGGTAATGGAGTATTGCCTGGGCTCAGCTTCTGACCTT; CTAGAAGTGCACAAGAAACCCCTTCAGGAGGTAGAGATCGCAGCTGTGACCCACGGGGCGCTTCAGGGC; CTGGCATATCTGCACTCCCACAACATGATCCATAGGGATGTGAAGGCTGGAAACATCCTGCTGTCAGAG; CCAGGGTTAGTGAAGCTAGGGGACTTTGGTTCTGCGTCCATCATGGCACCTGCCAACTCCTTCGTGGGC; ACCCCATACTGGATGGCACCCGAGGTGATCCTGGCCATGGATGAGGGGCAGTACGATGGCAAAGTGGAC; GTCTGGTCCTTGGGGATAACCTGCATCGAGCTGGCTGAACGGAAACCACCGCTCTTTAACATGAATGCG; ATGAGTGCCTTATACCACATTGCACAGAACGAATCCCCCGTGCTCCAGTCAGGACACTGGTCTGAGTAC; TTCCGGAATTTTGTCGACTCCTGTCTTCAGAAAATCCCTCAAGACAGACCAACCTCAGAGGTTCTCCTG; AAGCACCGCTTTGTGCTCCGGGAGCGGCCACCCACAGTCATCATGGACCTGATCCAGAGGACCAAGGAT; GCCGTGCGGGAGCTGGACAACCTGCAGTACCGCAAGATGAAGAAGATCCTGTTCCAAGAGGCACCCAAC; GGCCCTGGTGCCGAGGCCCCAGAGGAGGAAGAGGAGGCCGAGCCCTACATGCACCGGGCCGGGACTCTG; ACCAGCCTCGAGAGTAGCCACTCAGTGCCCAGCATGTCCATCAGCGCCTCCAGCCAGAGCAGCTCCGTC; AACAGCCTAGCAGATGCCTCAGACAACGAGGAAGAGGAGGAGGAGGAGGAGGAAGAGGAGGAGGAGGAA; GAAGGCCCTGAAGCCCGGGAGATGGCCATGATGCAGGAGGGGGAGCACACAGTCACCTCTCACAGCTCC; ATTATCCACCGGCTGCCGGGCTCTGACAACCTATATGATGACCCCTACCAGCCAGAGATAACCCCCAGC; CCTCTCCAGCCGCCTGCAGCCCCAGCTCCCACTTCCACCACCTCTTCCGCCCGCCGCCGGGCCTACTGC; CGTAACCGAGACCACTTTGCCACCATCCGAACCGCCTCCCTGGTCAGCCGTCAGATCCAGGAGCATGAG; CAGGACTCTGCGCTGCGGGAGCAGCTGAGCGGCTATAAGCGGATGCGACGACAGCACCAGAAGCAGCTG; CTGGCCCTGGAGTCACGGCTGAGGGGTGAACGGGAGGAGCACAGTGCACGGCTGCAGCGGGAGCTTGAG; GCGCAGCGGGCTGGCTTTGGGGCAGAGGCAGAAAAGCTGGCCCGGCGGCACCAGGCCATAGGTGAGAAG; GAGGCACGAGCTGCCCAGGCCGAGGAGCGGAAGTTCCAGCAGCACATCCTTGGGCAGCAGAAGAAGGAG; CTGGCTGCCCTGCTGGAGGCACAGAAGCGGACCTACAAACTTCGCAAGGAACAGCTGAAGGAGGAGCTC; CAGGAGAACCCCAGCACTCCCAAGCGGGAGAAGGCCGAGTGGCTGCTGCGGCAGAAGGAGCAGCTCCAG; CAGTGCCAGGCGGAGGAGGAAGCAGGGCTGCTGCGGCGGCAGCGCCAGTACTTTGAGCTGCAGTGTCGC; CAGTACAAGCGCAAGATGTTGCTGGCTCGGCACAGCCTGGACCAGGACCTGCTGCGGGAGGACCTGAAC; AAGAAGCAGACCCAGAAGGACTTGGAGTGTGCACTGCTGCTTCGGCAGCACGAGGCCACGCGGGAGCTG; GAGCTGCGGCAGCTCCAGGCCGTGCAGCGCACGCGGGCTGAGCTCACCCGCCTGCAGCACCAGACGGAG; CTGGGCAACCAGCTGGAGTACAACAAGCGGCGTGAGCAAGAGTTGCGGCAGAAGCATGCGGCCCAGGTT; CGCCAGCAGCCCAAGAGCCTCAAATCTAAGGAGCTGCAGATCAAGAAGCAGTTCCAGGAGACGTGTAAG; ATCCAGACTCGGCAGTACAAGGCTCTGCGAGCACACTTGCTGGAGACCACGCCCAAAGCTCAGCACAAG; AGCCTCCTTAAGCGGCTCAAGGAAGAGCAGACCCGCAAGCTGGCGATCTTGGCGGAGCAGTATGACCAG; TCCATCTCAGAGATGCTCAGCTCACAGGCGCTGCGGCTTGATGAGACCCAGGAGGCAGAGTTCCAGGCC; CTTCGGCAGCAGCTTCAACAGGAGCTGGAGCTGCTCAACGCTTACCAGAGCAAGATCAAGATCCGCACA; GAGAGCCAGCACGAGAGGGAGCTGCGGGAGCTGGAGCAGAGGGTCGCGCTGCGGCGGGCACTGCTGGAG; CAGCGGGTGGAAGAGGAGCTGCTGGCCCTGCAGACAGGACGCTCCGAGCGAATCCGCAGTCTGCTTGAG; CGGCAGGCCCGTGAGATCGAGGCCTTCGATGCGGAAAGCATGAGGCTGGGCTTCTCCAGCATGGCTCTG; GGGGGCATCCCGGCTGAAGCTGCTGCCCAGGGCTATCCTGCTCCACCCCCTGCCCCAGCCTGGCCCTCC; CGTCCCGTTCCCCGTTCTGGGGCACACTGGAGCCATGGCCCTCCTCCACCAGGCATGCCCCCTCCAGCC; TGGCGTCAGCCGTCTCTGCTGGCTCCCCCAGGCCCCCCAAACTGGCTGGGGCCCCCCACACAAAGTGGG; ACACCCCGTGGCGGAGCCCTGCTGCTGCTAAGAAACAGCCCCCAGCCCCTGCGGCGGGCAGCCTCGGGG; GGCAGTGGCAGTGAGAATGTGGGCCCCCCTGCTGCCGCGGTGCCCGGGCCCCTGAGCCGCAGCACCAGT; GTCGCTTCCCACATCCTCAATGGTTCTTCCCACTTCTAT;
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004783 unedited; ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACAGGGAAGGAGGTCTATATAAGCAGAGCTCGTTTAGTG; AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGACCCCTACCA; GGCCAGGCCCCACTCTCAGGGCCCCCAGGGGCCACCATGCCAGCTGGGGGCCGGGCCGGGAGCCTGAAGG; ACCCAGATGTGGCTGAGCTCTTCTTCAAGGATGACCCAGAAAAGCTCTTCTCTGACCTCCGGGAAATTGG; CCATGGCAGCTTTGGAGCCGTATACTTTGCCCGGGATGTCCGGAATAGTGAGGTGGTGGCCATCATGAAG; ATGTCCTACAGTGGGAAGCAGTCCAATGAGAAATGGCAAGACATCATCAAGGAGGTGCGGTTCTTACAGA; GCTCCGGCATCCCAACACCATTCAGTACCGGGGCTGTTACCTGAGGAGCACACGGCTTGCTGTAATGAGT; ATGCCTGGGCTCAGCTCTGACCTTCTAGATGCCACAGAACCCTCAGGAGGTAAAGAGATCCGCAGCTTGT; GACCCACCGGGCCGCTTCAGGCCTGGCATACTGCCTCCCACCACTTGATCATAGGATGTGAAGCCTGAAA; CATCTGCTGCCAGAGCCAGGGTTAGTGAACTCTAGGAACCTAGTCCGCTCACATCAGGGACACTCTGGCC; AATCTCCTTCTGGACACTATCTGTGGATGGCCCAGTGTATTCTGCTATGTAAGGGCTATCAATGGCCAAT; GGACCTTGCTCTGATAATGCCGTGCTAACGAGTCTGTCAAGCAGAACACGCGCATATAACGAATAGATCG; AATGAGTGCATAACATGTGCACGGACTATCCCGTCCATATAAGACAGCGTGTGATTCGAGATTTTGCAAC; ATGCTTCAAACTCCCGAGGAAC;
  Kinase Domain Sequence

  >SC323586 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation; CMGWGACCRTAAGCTSTTCTCTGACCTCCGGGAATTGGCCATGGCAGCTTTGGAGCCGTATACTTTGCCC; GGGATGTCCGGAATAGTGAGGTGGTGGCCATCATGAAGATGTCCTACAGTGGGAAGCAGTCCAATGAGAA; ATGGCAAGACATCATCAAGGAGGTGCGGTTCTTACAGAAGCTCCGGCATCCCAACACCATTCAGTACCGG; GGCTGTTACCTGAGGGAGCACACGGCTTGGCTGGTAATGGAGTAT;
• Restriction Sites: SgfI-MluI
• ACCN: NM_004783
• Insert Size: 3144 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004783.2
• RefSeq Size: 4625 bp
• RefSeq ORF: 3144 bp
• Locus ID: 9344
• UniProt ID: Q9UL54
• Cytogenetics: 16p11.2
• Domains: pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: MAPK signaling pathway
• MW: 119.2 kDa
• Summary: This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]; Transcript Variant: This variant (2) contains alternate exons at the 3' end compared to variant 1. This results in a shorter isoform (2, also known as PSK1-beta) with a distinct C-terminus compared to isoform 1.