ZBTB48 (NM_005341) Human Untagged Clone

SKU SC322351

ZBTB48 (untagged)-Human zinc finger and BTB domain containing 48 (ZBTB48)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ZBTB48
• Synonyms: HKR3; pp9964; TZAP; ZNF855
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322351 CGGACGCGTGGGCTCTTGCATACCCTCGCTTAGGCTGGCCGGGGTGTCACTTCTGCCTCC; CTGCCCTCCAGACCATGGACGGCTCCTTCGTCCAGCACAGTGTGAGGGTTCTGCAGGAGC; TCAACAAGCAGCGGGAGAAGGGCCAGTACTGCGACGCCACTCTGGACGTGGGGGGCCTGG; TGTTTAAGGCACACTGGAGTGTCCTTGCCTGCTGCAGTCACTTTTTCCAGAGCCTCTACG; GGGATGGCTCAGGGGGCAGTGTCGTCCTCCCTGCTGGCTTCGCTGAGATCTTTGGCCTCT; TGTTGGACTTTTTCTACACTGGTCACCTCGCTCTCACCTCAGGGAACCGGGATCAGGTGC; TCCTGGCAGCCAGGGAGTTGCGAGTGCCAGAGGCCGTAGAGCTGTGCCAGAGCTTCAAGC; CCAAAACTTCAGTGGGACAGGCAGCAGGTGGCCAGAGTGGGCTGGGGCCCCCTGCCTCCC; AGAATGTGAACAGCCACGTCAAGGAGCCGGCAGGCTTGGAAGAAGAGGAAGTTTCGAGGA; CTCTGGGTCTAGTCCCCAGGGATCAGGAGCCCAGAGGCAGTCATAGTCCTCAGAGGCCCC; AGCTCCATTCCCCAGCTCAGAGTGAGGGCCCCTCCTCCCTCTGTGGGAAACTGAAGCAGG; CCTTGAAGCCTTGTCCCCTTGAGGACAAGAAACCCGAGGACTGCAAAGTGCCCCCAAGGC; CCTTAGAGGCTGAAGGTGCCCAGCTGCAGGGCGGCAGTAATGAGTGGGAAGTGGTGGTTC; AAGTGGAGGATGATGGGGATGGCGATTACATGTCTGAGCCTGAGGCTGTGCTGACCAGGA; GGAAGTCAAATGTAATCCGAAAGCCCTGTGCAGCTGAGCCAGCCCTGAGCGCGGGCTCCC; TAGCAGCTGAGCCTGCTGAGAACAGAAAAGGTACAGCGGTGCCGGTCGAATGCCCCACAT; GTCATAAAAAGTTCCTCAGCAAATATTATCTAAAAGTCCACAACAGGAAACATACTGGGG; AGAAACCCTTTGAGTGTCCCAAATGTGGGAAGTGTTACTTTCGGAAGGAGAACCTCCTGG; AGCATGAAGCCCGGAATTGCATGAACCGCTCGGAACAGGTCTTCACGTGCTCTGTGTGCC; AGGAGACATTCCGCCGAAGGATGGAGCTGCGGGTGCACATGGTGTCTCACACAGGGGAGA; TGCCCTACAAGTGTTCCTCCTGCTCCCAGCAGTTCATGCAGAAGAAGGACTTGCAGAGCC; ACATGATCAAACTTCATGGAGCCCCCAAGCCCCATGCATGCCCCACCTGTGCCAAGTGCT; TCCTGTCTCGGACAGAGCTGCAGCTGCATGAAGCTTTCAAGCACCGTGGTGAGAAGCTGT; TTGTGTGTGAGGAGTGTGGGCACCGGGCCTCGAGCCGGAATGGCCTGCAGATGCACATCA; AGGCCAAGCACAGGAATGAGAGGCCACACGTATGTGAGTTCTGCAGCCACGCCTTCACCC; AAAAGGCCAATCTCAACATGCACCTGCGCACACACACGGGTGAGAAGCCCTTCCAGTGCC; ACCTCTGTGGCAAGACCTTCCGAACCCAAGCCAGCCTGGACAAGCACAACCGCACCCACA; CCGGGGAAAGGCCCTTCAGTTGCGAGTTCTGTGAACAGCGCTTCACTGAGAAGGGGCCCC; TCCTGAGGCACGTGGCCAGCCGCCATCAGGAGGGCCGGCCCCACTTCTGCCAGATATGCG; GCAAGACCTTCAAAGCCGTGGAGCAACTGCGTGTGCACGTCAGACGGCACAAGGGGGTGA; GGAAGTTTGAGTGCACCGAGTGTGGCTACAAGTTTACCCGACAGGCCCACCTGCGGAGGC; ACATGGAGATCCACGACCGGGTAGAGAACTACAACCCGCGGCAGCGCAAGCTCCGCAACC; TGATCATCGAGGACGAGAAGATGGTGGTGGTGGCGCTGCAGCCGCCTGCAGAGCTGGAGG; TGGGCTCGGCGGAGGTCATTGTGGAGTCCCTGGCCCAGGGCGGCCTGGCCTCCCAGCTCC; CCGGCCAGAGACTGTGTGCAGAGGAGAGCTTCACCGGCCCAGGTGTCCTGGAGCCCTCCC; TCATCATCACAGCTGCTGTCCCCGAGGACTGTGACACATAGCCCACTCTGGCCACCAGAG; CCCACTTGGCCCCACCCCTCAATAAACCGTGTGGCTTTGGACTCTCGTAAAAAAAAAAAA; AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAA
• Restriction Sites: Please inquire
• ACCN: NM_005341
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005341.1, NP_005332.1
• RefSeq Size: 2289 bp
• RefSeq ORF: 2067 bp
• Locus ID: 3104
• UniProt ID: P10074
• Cytogenetics: 1p36.31
• Domains: BTB, zf-C2H2
• Protein Families: Transcription Factors
• Summary: Telomere-binding protein that acts as a regulator of telomere length (PubMed:28500257, PubMed:28082411). Directly binds the telomeric double-stranded 5'-TTAGGG-3' repeat (PubMed:28500257, PubMed:28082411). Preferentially binds to telomeres that have a low concentration of shelterin complex and acts as a regulator of telomere length by initiating telomere trimming, a process that prevents the accumulation of aberrantly long telomeres (PubMed:28082411). Also acts as a transcription regulator that binds to promoter regions (PubMed:7969177, PubMed:24382891, PubMed:28500257). Regulates expression of a small subset of genes, including MTFP1 (PubMed:28500257). Regulates expression the J and/or S elements in MHC II promoter (PubMed:7969177). Acts as a negative regulator of cell proliferation by specifically activating expression of ARF, a tumor suppressor isoform of CDKN2A (PubMed:24382891).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.