ODAD4 (NM_031421) Human Untagged Clone

SKU SC322025

TTC25 (untagged)-Human tetratricopeptide repeat domain 25 (TTC25)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ODAD4
• Synonyms: TTC25
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322025 TAGAGGTTCTCCAGCTTTTCTTTGATTGTCTCTGCTTTAGCGTCTCTAAATCCGGTCACC; ATGTCGGACCCCGAAGGCGAGACCTTGCGAAGCACCTTTCCCTCTTATATGGCCGAAGGC; GAGCGGCTCTACCTGTGCGGGGAATTTTCTAAAGCCGCGCAGAGCTTCAGCAACGCTCTT; TACCTTCAGGATGGAGACAAGAACTGCCTGGTTGCTCGCTCAAAGTGCTTCCTGAAGATG; GGAGACTTGGAGAGATCCCTGAAGGATGCTGAGGCTTCGCTCCAGAGTGACCCAGCTTTC; TGTAAGGGGATTTTGCAAAAGGCTGAGACACTGTACACCATGGGAGACTTTGAGTTTGCC; TTGGTATTCTATCATCGAGGCTACAAGCTGAGGCCTGATCGGGAATTCAGAGTTGGCATT; CAGAAAGCCCAGGAAGCCATCAACAACTCAGTGGGAAGTCCTTCTTCCATTAAGCTGGAG; AACAAAGGGGACCTCTCCTTCTTAAGCAAGCAGGCTGAGAATATAAAAGCCCAGCAGAAG; CCTCAGCCCATGAAACACCTCTTACACCCCACCAAGGGAGAGCCCAAGTGGAAGGCCTCG; CTCAAGAGTGAGAAGACTGTCCGCCAGCTTCTGGGGGAGCTCTACGTGGACAAAGAGTAT; TTGGAGAAGCTCCTATTGGATGAAGACCTGATCAAAGGCACCATGAAGGGTGGCCTGACT; GTGGAGGACCTCATCATGACGGGCATCAACTACCTGGATACTCACAGCAACTTCTGGAGG; CAGCAGAAGCCGATCTACGCCAGGGAGCGGGACCGGAAGCTGATGCAAGAGAAATGGCTG; CGGGACCACAAACGCCGTCCCTCACAGACAGCCCATTACATCCTCAAGAGCCTGGAGGAC; ATTGATATGTTGCTCACAAGTGGCAGTGCTGAAGGGAGTCTTCAGAAAGCTGAGAAAGTG; CTGAAGAAGGTACTGGAATGGAACAAGGAAGAGGTACCCAACAAGGATGAACTGGTTGGA; AACTTGTATAGCTGCATAGGGAATGCCCAGATTGAGCTGGGGCAGATGGAGGCAGCCCTG; CAGAGCCACAGAAAGGACCTGGAGATCGCCAAGGAATATGACCTTCCTGATGCAAAATCG; AGAGCCCTTGACAACATTGGCAGAGTTTTTGCCAGAGTTGGGAAATTCCAGCAAGCCATT; GACACGTGGGAAGAAAAGATCCCTCTGGCAAAAACCACCCTGGAGAAGACCTGGCTGTTC; CACGAGATCGGCCGCTGCTACTTGGAGCTGGACCAGGCCTGGCAGGCCCAGAATTATGGC; GAGAAGTCCCAGCAGTGTGCCGAGGAGGAAGGGGACATTGAGTGGCAACTGAATGCCAGT; GTTCTGGTGGCCCAGGCACAAGTGAAGCTGAGAGACTTCGAGTCAGCCGTGAACAATTTT; GAGAAGGCCCTGGAGAGAGCAAAGCTTGTGCATAACAACGAGGCGCAGCAGGCCATCATC; AGTGCCTTGGACGATGCCAACAAGGGTATCATCAGAGAACTGAGGAAAACCAACTACGTG; GAGAATCTCAAAGAAAAAAGCGAGGGAGAAGCTTCACTGTATGAAGATAGAATAATAACA; AGAGAGAAGGACATGAGGAGAGTGAGAGATGAGCCCGAGAAGGTGGTGAAGCAGTGGGAC; CATAGTGAGGATGAGAAAGAGACAGATGAGGACGATGAGGCTTTTGGGGAAGCTCTGCAG; AGCCCAGCAAGCGGAAAGCAGAGTGTGGAAGCAGGAAAAGCCAGAAGCGATTTGGGAGCA; GTTGCCAAGGGCCTGTCAGGAGAATTAGGCACAAGATCAGGAGAAACAGGCAGGAAGCTA; CTAGAAGCTGGCAGAAGAGAGTCAAGAGAAATTTATAGGAGGCCTTCGGGAGAATTAGAG; CAAAGACTCTCAGGAGAATTCAGCAGACAGGAACCAGAAGAACTAAAGAAACTTTCAGAA; GTGGGCAGAAGAGAGCCAGAAGAACTGGGAAAAACACAATTTGGAGAAATAGGAGAAACG; AAAAAAAACAGGAAATGAGATGGAAAAGGAATATGAATGAAGCCATCGGTAGAGATGAGG; ATCAGGAAGCTGGTGTTCAGAGGGATCATGGGATTTTATTAAACTGGATTTTCAAGCGAT; TTGTCTGTTATAGGAAAAATGAGGGTTTTACTTCTGCTGCTTTCCATCACTATTTTGCCA; TTAAATAGGTGTCTTTCACTCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_031421
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_031421.1, NP_113609.1
• RefSeq Size: 2277 bp
• RefSeq ORF: 2019 bp
• Locus ID: 83538
• UniProt ID: Q96NG3
• Cytogenetics: 17q21.2
• Domains: TPR
• Summary: This gene encodes a tetratricopeptide repeat domain-containing protein that localizes to ciliary axonmenes and plays a role in the docking of the outer dynein arm to cilia. Mutations in this gene cause severely reduced ciliary motility and the disorder CILD35 (ciliary dyskinesia,primary, 35). Primary ciliary dyskinesia is often associated with recurrent respiratory infections, immotile spermatozoa, and situs inversus; an inversion in left-right body symmetry. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2017]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.