THOC7 (NM_025075) Human Untagged Clone

SKU
SC321996
THOC7 (untagged)-Human THO complex 7 homolog (Drosophila) (THOC7)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol THOC7
Synonyms fSAP24; hTREX30; NIF3L1BP1
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for SC321996 GCCATGGGAGCCGTGACTGACGACGAAGTTATACGGAAGCGTCTCCTCATTGATGGAGAT
GGTGCTGGAGATGATCGGAGAATTAATCTGCTAGTGAAGAGTTTCATTAAATGGTGCAAC
TCTGGGTCCCAGGAAGAGGGATATAGCCAGTACCAACGTATGCTGAGCACGCTGTCTCAA
TGTGAATTTTCAATGGGCAAAACTTTACTAGTATATGATATGAATCTCAGAGAAATGGAA
AATTATGAAAAAATTTACAAGGAAATAGAATGTAGCATAGCTGGAGCACATGAAAAAATT
GCTGAGTGCAAAAAGCAAATTCTTCAAGCAAAACGAATACGAAAAAATCGCCAAGAATAT
GATGCTTTGGCAAAAGTGATTCAGCACCATCCAGACAGGCATGAGACATTAAAGGAACTA
GAGGCTCTGGGAAAAGAATTAGAGCATCTTTCACACATTAAAGAAAGTGTTGAAGATAAG
CTGGAATTGAGACGGAAACAGTTTCATGTTCTTCTTAGTACCATCCATGAACTTCAGCAA
ACATTGGAAAATGATGAAAAACTCTCAGAGGTAGAAGAAGCTCAGGAAGCAAGCATGGAA
ACAGATCCTAAGCCATAGACAGGCTAATTGCCCACCACTCCCAGGAATATTGAAATAGCT
ACATGACCATAATGTGTTTAAAATGTGGTATGCTCTTGAGATATTTAAAGTTTTGGCAGT
AAAATACTCTGTTTTTAAGTATGAATGTATTTCATTCATATTTCCTCTCACAAAGGAAAA
TGACTTCAGTATAGATTTGTTTTTATTAAAATGCATTTTTTATTCTTAAGTGGTAGGAAG
CAACATCCAAAAATGCTTAATAAAATGCTTTTAAGCTGCAAAAAAAAAAAAAAAAAAAAA
AAA
Restriction Sites Please inquire |
ACCN NM_025075
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025075.1, NP_079351.1
RefSeq Size 963 bp
RefSeq ORF 615 bp
Locus ID 80145
UniProt ID Q6I9Y2
Cytogenetics 3p14.1
Summary Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_025075" in other vectors (4)
SKU Description Size Price
RC209500 THOC7 (Myc-DDK-tagged)-Human THO complex 7 homolog (Drosophila) (THOC7) 10 ug
$300.00
RC209500L3 Lenti ORF clone of Human THO complex 7 homolog (Drosophila) (THOC7), Myc-DDK-tagged 10 ug
$600.00
RC209500L4 Lenti ORF clone of Human THO complex 7 homolog (Drosophila) (THOC7), mGFP tagged 10 ug
$600.00
RG209500 THOC7 (tGFP-tagged) - Human THO complex 7 homolog (Drosophila) (THOC7) 10 ug
$489.00 $500.00

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