Renin (REN) (NM_000537) Human Untagged Clone
$457.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | Renin |
| Synonyms | ADTKD4; HNFJ2; RTD |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_000537 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000537.2, NP_000528.1 |
| RefSeq Size | 1461 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 5972 |
| UniProt ID | P00797 |
| Cytogenetics | 1q32.1 |
| Protein Families | Druggable Genome, Secreted Protein |
| Protein Pathways | Renin-angiotensin system |
| Summary | This gene encodes renin, an aspartic protease that is secreted by the kidneys. Renin is a part of the renin-angiotensin-aldosterone system involved in regulation of blood pressure, and electrolyte balance. This enzyme catalyzes the first step in the activation pathway of angiotensinogen by cleaving angiotensinogen to form angiotensin I, which is then converted to angiotensin II by angiotensin I converting enzyme. This cascade can result in aldosterone release, narrowing of blood vessels, and increase in blood pressure as angiotension II is a vasoconstrictive peptide. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause hyperuricemic nephropathy familial juvenile 2, familial hyperproreninemia, and renal tubular dysgenesis. provided by RefSeq, May 2020 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000537" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208382 | REN (Myc-DDK-tagged)-Human renin (REN) | 10 ug |
$457.00
|
|
| RC208382L1 | Lenti ORF clone of Human renin (REN), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC208382L2 | Lenti ORF clone of Human renin (REN), mGFP tagged | 10 ug |
$757.00
|
|
| RC208382L3 | Lenti ORF clone of Human renin (REN), Myc-DDK-tagged | 10 ug |
$757.00
|
|
| RC208382L4 | Lenti ORF clone of Human renin (REN), mGFP tagged | 10 ug |
$757.00
|
|
| RG208382 | REN (tGFP-tagged) - Human renin (REN) | 10 ug |
$489.00
$657.00
|
|
Citations
Leader peptide or pro-segment mutants of renin are misrouted to mitochondria in autosomal dominant tubulointerstitial kidney disease
Schaeffer, De Fusco, Pasqualetto et al
Dis Model Mech (2023) 16 (6) DOI: 10.1242/dmm.049963
Schaeffer, De Fusco, Pasqualetto et al
Dis Model Mech (2023) 16 (6) DOI: 10.1242/dmm.049963
Cited by 4
AI Summary: OriGene's human cDNA renin untagged clone (SC321832) was used as the template for generating mutant and wild type renin constructs, enabling the study of how specific mutations affect renin's cellular localization and contribute to kidney disease.
Snippet: … MATERIALS AND METHODS Constructs Human cDNA of a untagged renin clone (SC321832) was purchased from Origene (Rockville, MD, USA). Renin cDNA was subcloned in pcDNA3.1 (Thermo Fisher Scientific, Waltham, MA, USA) and HA was inserted at the C-terminal (Schaeffer et al., 2019). The indicated mutations;Human cDNA renin untagged clone (SC321832) was purchased from Origene (Rockville, MD). Renin cDNA was subcloned in pcDNA3.1 (Thermofisher, Waltham, MA) and HA was inserted at the C-terminal (Schaeffer et al., 2019). The indicated mutations were introduced in the pcDNA-hRenHA construct using the QuikChange Lightning Site-Directed. …
Showing 1-1 of 1 papers.
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