GLRB (NM_000824) Human Untagged Clone

SKU SC321743

GLRB (untagged)-Human glycine receptor, beta (GLRB), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GLRB
• Synonyms: HKPX2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000824.2 CTGGGACGCAGCTGCCCCCGCTCGGCGCCCGCTGCACCCTTAGCAGCCACTGCCACCTGG; GCCCGGAGCCTCCACGATCTCGCCCGGCGATTGTGGGCAGGGGCGCCTCCGGATCGATCT; TCTGAAATTCAAGTTTTCAAGATGAAGTTTTTATTGACAACTGCCTTTTTAATTTTAATT; TCCTTGTGGGTGGAAGAAGCCTATTCTAAGGAAAAGTCTTCAAAGAAAGGGAAGGGGAAA; AAGAAGCAGTATCTATGCCCATCTCAGCAGTCAGCAGAGGACCTTGCCCGAGTACCTGCC; AACTCCACTAGCAATATCTTGAACAGGTTATTGGTCAGTTATGATCCCAGGATAAGACCA; AACTTCAAAGGCATTCCTGTTGATGTAGTAGTCAACATTTTTATTAACAGTTTTGGATCC; ATTCAAGAAACAACAATGGACTATAGAGTTAACATCTTCCTGAGACAAAAATGGAATGAC; CCCAGGCTGAAGCTCCCCAGTGATTTTAGGGGTTCAGATGCACTGACAGTGGATCCAACA; ATGTACAAGTGTTTATGGAAACCTGATTTATTTTTTGCAAATGAAAAAAGTGCCAATTTT; CATGATGTGACCCAGGAAAACATCCTCCTCTTTATTTTTCGTGATGGAGATGTCCTTGTC; AGCATGAGGTTATCTATTACTCTTTCATGCCCTTTGGACTTGACATTGTTTCCCATGGAT; ACACAACGTTGCAAGATGCAACTGGAGAGCTTTGGTTACACAACTGATGATTTACGATTT; ATCTGGCAGTCAGGAGATCCTGTGCAATTAGAAAAAATTGCCTTGCCTCAATTTGATATC; AAAAAGGAAGATATTGAATATGGTAACTGTACAAAATACTATAAAGGCACGGGCTACTAC; ACATGCGTGGAAGTCATCTTCACCCTGAGGAGGCAGGTCGGCTTTTACATGATGGGGGTC; TACGCCCCAACCCTGCTCATTGTTGTTCTCTCCTGGCTTTCCTTCTGGATCAACCCGGAC; GCGAGTGCTGCCAGAGTGCCCCTGGGTATCTTCTCAGTCCTCAGCTTGGCCTCTGAGTGC; ACAACCCTTGCCGCTGAGCTTCCCAAAGTTTCCTATGTGAAGGCTCTTGATGTTTGGCTT; ATTGCTTGCCTTCTCTTTGGGTTTGCTTCCCTGGTGGAGTATGCAGTTGTCCAGGTGATG; CTGAACAACCCCAAAAGGGTTGAAGCTGAAAAAGCCAGAATTGCTAAGGCTGAGCAAGCA; GATGGAAAAGGTGGAAATGTGGCTAAAAAGAATACTGTGAATGGAACAGGGACTCCTGTT; CATATTAGCACTTTGCAGGTTGGTGAGACCAGATGCAAAAAAGTTTGTACTTCTAAGTCT; GATCTGAGATCTAATGACTTCAGCATTGTTGGAAGCTTACCAAGAGATTTTGAACTATCC; AATTATGACTGCTATGGAAAACCCATTGAAGTTAACAACGGACTTGGGAAATCTCAGGCT; AAGAACAACAAGAAGCCTCCCCCTGCGAAACCTGTTATTCCAACAGCAGCAAAGCGAATT; GATCTTTATGCAAGAGCATTGTTTCCTTTCTGCTTCTTGTTCTTCAATGTTATATATTGG; TCTATATATTTATGATAAATCTTTTCCATTTGTACAAAATAAAATTCCATTTCATTGTGA; CCTACTCCTTTCATAAATGCCAATCTGTGAGAACTTTTGAATTTTCATAGCAACATTGCA; TTTTGGATGCCATTTGATTGTAATAAAACTGTGGCACCTTAATTTTGAATGGCAGCATGA; TCATGTAATATCTGTGCTCTAATAACGATGTATATATGTATAGTGAACATATTGCTTAGT; AACAAATGAAGGACAAGCATACTACATAATATAATCCATACAATTCTCTTCAGTTAGTGT; AAACTGCAAATACTACAGATAATTCTGATAATAAAATGATATGCACGCTGAATCCTGCTA; TGGTCACCATTCTAATGTATGTAGTATTTCAAATTTCCTTCCTTGTAACTTTCAAAGAAA; GCCATCTTATTCTTGTAATTTTAGATGGTATTATCACAGATTTAAAAAGGTTGTATTACA; TATTGTTTAAACTTTGTAAGTAGAAATATATCTGTTATAATTATACAGGCTCTGTGGAGA; AATAAAGTTCAAAATATATTAATTTGTAAAATCAGCTCGTTTTAAAGTGTGCTTGTGTTG; TCAAAAATATCAGATAGTAATACACAGTGAGCATTTTTAAACAAAGGGAAACCTATATTT; ATGTAACTGTATACTGAATTCTGACAAAATAAAAAAAGATACCTTATTGACGAAATATTT; AGGATAAACAAAATTCTATTTAATCCACCTTAAAACCTAAATGTATTTTCATGGATTTCA; TTTGTTGGTACATATTACACAAAACATTGTGCCTTAAAATGAGTCATACATCTTTTAAAT; TGGAATGCAGTAATAGATATGTGATTTTACATCATTTTTAAGAAACCAAGGGGAAGTAAT; AAGTTGAAAAAGAAATCCATAACTATTAAAAGATTTTAACTTTTTTATTTTATTAAAATG; CTTGCATATTTTAAGTAAAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000824
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000824.2, NP_000815.1
• RefSeq Size: 2649 bp
• RefSeq ORF: 1494 bp
• Locus ID: 2743
• UniProt ID: P48167
• Cytogenetics: 4q32.1
• Domains: Neur_chan_LBD, Neur_chan_memb
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Summary: This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (1, also known as variant A) represents the longest transcript and encodes the longer isoform (A). Both variants 1 and 2 encode the same isoform.