ATP1A4 (NM_001001734) Human Untagged Clone

SKU SC321721

ATP1A4 (untagged)-Human ATPase, Na+/K+ transporting, alpha 4 polypeptide (ATP1A4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: ATP1A4
• Synonyms: ATP1A1; ATP1AL2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001001734.1 GAAGCTCACTCCGCCTCCTCACTCTCACTGCATTTCCCACCTTCCTGTGGGCCTTGCGGC; ATCTTCATCACTGAGGCACCTGGTTACTCTTCACCTCTTGTTTCCTGCCCTCACTGCATT; CCCTCACCTCTACCTTTTTATCCTTCCACCCTAGGCTTCTCTCCTCCCTCTTCCCTCACT; CCTGACTCTTCCTCTTCCCAGCGGACGGCTGGAGGACCGCTCAGTCTCTCCTCTCTCACT; TCCCTTCCTCTCTCTCACCTTCACCACCCAACACCTCCCTCCCTGCCTCTTTCTTTCTGC; TCCCTCATTCTCTCCCCACCACTCTCTTCTCGTGGCCCCCTTGCCTGCGCGCCCTCTTCC; CTTCCCCTTGCCTCACTCTCTCAGCTTTCTTCCCACAGTTGAGCTCGGGCAGCTCTTTCT; GGGGATAGCTATGGGGCTTTGGGGGAAGAAAGGGACAGTGGCTCCCCATGACCAGAGTCC; AAGACGAAGACCTAAAAAAGGGCTTATCAAGAAAAAAATGGTGAAGAGGGAAAAACAGAA; GCGCAATATGGAGGAACTGAAGAAGGAAGTGGTCATGGATGATCACAAATTAACCTTGGA; AGAGCTGAGCACCAAGTACTCCGTGGACCTGACAAAGGGCCATAGCCACCAAAGGGCAAA; GGAAATCCTGACTCGAGGTGGACCCAATACTGTTACCCCACCCCCCACCACTCCAGAATG; GGTCAAATTCTGTAAGCAACTGTTCGGAGGCTTCTCCCTCCTACTATGGACTGGGGCCAT; TCTCTGCTTTGTGGCCTACAGCATCCAGATATATTTCAATGAGGAGCCTACCAAAGACAA; CCTCTACCTGAGCATCGTACTGTCCGTCGTGGTCATCGTCACTGGCTGCTTCTCCTATTA; TCAGGAGGCCAAGAGCTCCAAGATCATGGAGTCTTTTAAGAACATGGTGCCTCAGCAAGC; TCTGGTAATTCGAGGAGGAGAGAAGATGCAAATTAATGTACAAGAGGTGGTGTTGGGAGA; CCTGGTGGAAATCAAGGGTGGAGACCGAGTCCCTGCTGACCTCCGGCTTATCTCTGCACA; AGGATGTAAGGTGGACAACTCATCCTTGACTGGGGAGTCAGAACCCCAGAGCCGCTCCCC; TGACTTCACCCATGAGAACCCTCTGGAGACCCGAAACATCTGCTTCTTTTCCACCAACTG; TGTGGAAGGAACCGCCCGGGGTATTGTGATTGCTACGGGAGACTCCACAGTGATGGGCAG; AATTGCCTCCCTGACGTCAGGCCTGGCGGTTGGCCAGACACCTATCGCTGCTGAGATCGA; ACACTTCATCCATCTGATCACTGTGGTGGCCGTCTTCCTTGGTGTCACTTTTTTTGCGCT; CTCACTTCTCTTGGGCTATGGTTGGCTGGAGGCTATCATTTTTCTCATTGGCATCATTGT; GGCCAATGTGCCTGAGGGGCTGTTGGCTACAGTCACTGTGTGCCTGACCCTCACAGCCAA; GCGCATGGCACGGAAGAACTGCCTGGTGAAGAACCTGGAGGCGGTGGAGACGCTGGGCTC; CACGTCCACCATCTGCTCAGACAAGACGGGCACCCTCACCCAGAACCGCATGACCGTCGC; CCACATGTGGTTTGATATGACCGTGTATGAGGCCGACACCACTGAAGAACAGACTGGAAA; AACATTTACCAAGAGCTCTGATACCTGGTTTATGCTGGCCCGAATCGCTGGCCTCTGCAA; CCGGGCTGACTTTAAGGCTAATCAGGAGATCCTGCCCATTGCTAAGAGGGCCACAACAGG; TGATGCTTCCGAGTCAGCCCTCCTCAAGTTCATCGAGCAGTCTTACAGCTCTGTGGCGGA; GATGAGAGAGAAAAACCCCAAGGTGGCAGAGATTCCCTTTAATTCTACCAACAAGTACCA; GATGTCCATCCACCTTCGGGAGGACAGCTCCCAGACCCACGTACTGATGATGAAGGGTGC; TCCGGAGAGGATCTTGGAGTTTTGTTCTACCTTTCTTCTGAATGGGCAGGAGTACTCAAT; GAACGATGAAATGAAGGAAGCCTTCCAAAATGCCTATTTAGAACTGGGAGGTCTGGGGGA; ACGTGTGCTAGGCTTCTGCTTCTTGAATCTGCCTAGCAGCTTCTCCAAGGGATTCCCATT; TAATACAGATGAAATAAATTTCCCCATGGACAACCTTTGTTTTGTGGGCCTCATATCCAT; GATTGACCCTCCCCGAGCTGCAGTGCCTGATGCTGTGAGCAAGTGTCGCAGTGCAGGAAT; TAAGGTGATCATGGTAACAGGAGATCATCCCATTACAGCTAAGGCCATTGCCAAGGGTGT; GGGCATCATCTCAGAAGGCACTGAGACGGCAGAGGAAGTCGCTGCCCGGCTTAAGATCCC; TATCAGCAAGGTCGATGCCAGTGCTGCCAAAGCCATTGTGGTGCATGGTGCAGAACTGAA; GGACATACAGTCCAAGCAGCTTGATCAGATCCTCCAGAACCACCCTGAGATCGTGTTTGC; TCGGACCTCCCCTCAGCAGAAGCTCATCATTGTCGAGGGATGTCAGAGGCTGGGAGCCGT; TGTGGCCGTGACAGGTGACGGGGTGAACGACTCCCCTGCGCTGAAGAAGGCTGACATTGG; CATTGCCATGGGCATCTCTGGCTCTGACGTCTCTAAGCAGGCAGCCGACATGATCCTGCT; GGATGACAACTTTGCCTCCATCGTCACGGGGGTGGAGGAGGGCCGCCTGATCTTTGACAA; CCTGAAGAAATCCATCATGTACACCCTGACCAGCAACATCCCCGAGATCACGCCCTTCCT; GATGTTCATCATCCTCGGTATACCCCTGCCTCTGGGAACCATAACCATCCTCTGCATTGA; TCTCGGCACTGACATGGTCCCTGCCATCTCCTTGGCTTATGAGTCAGCTGAAAGCGACAT; CATGAAGAGGCTTCCAAGGAACCCAAAGACGGATAATCTGGTGAACCACCGTCTCATTGG; CATGGCCTATGGACAGATTGGGATGATCCAGGCTCTGGCTGGATTCTTTACCTACTTTGT; AATCCTGGCTGAGAATGGTTTTAGGCCTGTTGATCTGCTGGGCATCCGCCTCCACTGGGA; AGATAAATACTTGAATGACCTGGAGGACAGCTACGGACAGCAGTGGACCTATGAGCAACG; AAAAGTTGTGGAGTTCACATGCCAAACGGCCTTTTTTGTCACCATCGTGGTTGTGCAGTG; GGCGGATCTCATCATCTCCAAGACTCGCCGCAACTCACTTTTCCAGCAGGGCATGAGAAA; CAAAGTCTTAATATTTGGGATCCTGGAGGAGACACTCTTGGCTGCATTTCTGTCCTACAC; TCCAGGCATGGACGTGGCCCTGCGAATGTACCCACTCAAGATAACCTGGTGGCTCTGTGC; CATTCCCTACAGTATTCTCATCTTCGTCTATGATGAAATCAGAAAACTCCTCATCCGTCA; GCACCCGGATGGCTGGGTGGAAAGGGAGACGTACTACTAAACTCAGCAGATGAAGAGCTT; CATGTGACACAGGGGTGTTGTGAGAACTGGGATGGGGCCAGAGATTATAAGTTTGACACA; ACAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001001734
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001001734.1, NP_001001734.1
• RefSeq Size: 951 bp
• RefSeq ORF: 498 bp
• Locus ID: 480
• UniProt ID: Q13733
• Cytogenetics: 1q23.2
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction
• Summary: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) contains a distinct 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2), which has not been experimentally confirmed, has a shorter N-terminus when compared to isoform 1.