Cullin 2 (CUL2) (NM_003591) Human Untagged Clone
$1,024.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | Cullin 2 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_003591 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_003591.2, NP_003582.2 |
| RefSeq Size | 2910 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 8453 |
| UniProt ID | Q13617 |
| Cytogenetics | 10p11.21 |
| Domains | CULLIN |
| Protein Families | Druggable Genome |
| Protein Pathways | Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis |
| Summary | Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).UniProtKB/Swiss-Prot Function Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Variants 3 and 4 both encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_003591" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203304 | CUL2 (Myc-DDK-tagged)-Human cullin 2 (CUL2), transcript variant 3 | 10 ug |
$1,023.00
|
|
| RC203304L1 | Lenti ORF clone of Human cullin 2 (CUL2), transcript variant 3, Myc-DDK-tagged | 10 ug |
$1,323.00
|
|
| RC203304L2 | Lenti ORF clone of Human cullin 2 (CUL2), transcript variant 3, mGFP tagged | 10 ug |
$1,323.00
|
|
| RC203304L3 | Lenti ORF clone of Human cullin 2 (CUL2), transcript variant 3, Myc-DDK-tagged | 10 ug |
$1,323.00
|
|
| RC203304L4 | Lenti ORF clone of Human cullin 2 (CUL2), transcript variant 3, mGFP tagged | 10 ug |
$1,323.00
|
|
| RG203304 | CUL2 (tGFP-tagged) - Human cullin 2 (CUL2), transcript variant 3 | 10 ug |
$1,223.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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