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KAP1 (TRIM28) (NM_005762) Human Untagged Clone

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SKU
SC321588
TRIM28 (untagged)-Human tripartite motif containing 28 (TRIM28)
  $765.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol KAP1
Synonyms KAP1; PPP1R157; RNF96; TF1B; TIF1B
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_005762.2 GGCGCGCGGGCGAGCGGTTGTGCTTGTGCTTGTGGCGCGTGGTGCGGGTTTCGGCGGCGG
CTGAGGAAGAAGCGCGGGCGGCGCCTTCGGGAGGCGAGCAGGCAGCAGTTGGCCGTGCCG
TAGCAGCGTCCCGCGCGCGGCGGGCAGCGGCCCAGGAGGCGCGTGGCGGCGCTCGGCCTC
GCGGCGGCGGCGGCGGCAGCGGCCCAGCAGTTGGCGGCGAGCGCGTCTGCGCCTGCGCGG
CGGGCCCCGCGCCCCTCCTCCCCCCCTGGGCGCCCCCGGCGGCGTGTGAATGGCGGCCTC
CGCGGCGGCAGCCTCGGCAGCAGCGGCCTCGGCCGCCTCTGGCAGCCCGGGCCCGGGCGA
GGGCTCCGCTGGCGGCGAAAAGCGCTCCACCGCCCCTTCGGCCGCAGCCTCGGCCTCTGC
CTCAGCCGCGGCGTCGTCGCCCGCGGGGGGCGGCGCCGAGGCGCTGGAGCTGCTGGAGCA
CTGCGGCGTGTGCAGAGAGCGCCTGCGACCCGAGAGGGAGCCCCGCCTGCTGCCCTGTTT
GCACTCGGCCTGTAGTGCCTGCTTAGGGCCCGCGGCCCCCGCCGCCGCCAACAGCTCGGG
GGACGGCGGGGCGGCGGGCGACGGCACCGTGGTGGACTGTCCCGTGTGCAAGCAACAGTG
CTTCTCCAAAGACATCGTGGAGAATTATTTCATGCGTGATAGTGGCAGCAAGGCTGCCAC
CGACGCCCAGGATGCGAACCAGTGCTGCACTAGCTGTGAGGATAATGCCCCAGCCACCAG
CTACTGTGTGGAGTGCTCGGAGCCTCTGTGTGAGACCTGTGTAGAGGCGCACCAGCGGGT
GAAGTACACCAAGGACCATACTGTGCGCTCTACTGGGCCAGCCAAGTCTCGGGATGGTGA
ACGTACTGTCTATTGCAACGTACACAAGCATGAACCCCTTGTGCTGTTTTGTGAGAGCTG
TGATACTCTCACCTGCCGAGACTGCCAGCTCAATGCCCACAAGGACCACCAGTACCAGTT
CTTAGAGGATGCAGTGAGGAACCAGCGCAAGCTCCTGGCCTCACTGGTGAAGCGCCTTGG
GGACAAACATGCAACATTGCAGAAGAGCACCAAGGAGGTTCGCAGCTCAATCCGCCAGGT
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGCAGATCATGAA
GGAGCTGAATAAGCGGGGCCGTGTGCTGGTCAATGATGCCCAGAAGGTGACTGAGGGGCA
GCAGGAGCGCCTGGAGCGGCAGCACTGGACCATGACCAAGATCCAGAAGCACCAGGAGCA
CATTCTGCGCTTTGCCTCTTGGGCTCTGGAGAGTGACAACAACACAGCCCTTTTGCTTTC
TAAGAAGTTGATCTACTTCCAGCTGCACCGGGCCCTCAAGATGATTGTGGATCCCGTGGA
GCCACATGGCGAGATGAAGTTTCAGTGGGACCTCAATGCCTGGACCAAGAGTGCCGAGGC
CTTTGGCAAGATTGTGGCAGAGCGTCCTGGCACTAACTCAACAGGCCCTGCACCCATGGC
CCCTCCAAGAGCCCCAGGGCCCCTGAGCAAGCAGGGCTCTGGCAGCAGCCAGCCCATGGA
GGTGCAGGAAGGCTATGGCTTTGGGTCAGGAGATGATCCCTACTCAAGTGCAGAGCCCCA
TGTGTCAGGTGTGAAACGGTCCCGCTCAGGTGAGGGCGAGGTGAGCGGCCTTATGCGCAA
GGTGCCACGAGTGAGCCTTGAACGCCTGGACCTGGACCTCACAGCTGACAGCCAGCCACC
CGTCTTCAAGGTCTTCCCAGGCAGTACCACTGAGGACTACAACCTTATTGTTATTGAACG
TGGCGCTGCCGCTGCAGCTACCGGCCAGCCAGGGACTGCGCCTGCAGGAACCCCTGGTGC
CCCACCCCTGGCTGGCATGGCCATTGTCAAGGAGGAGGAGACGGAGGCTGCCATTGGAGC
CCCTCCTACTGCCACTGAGGGCCCTGAGACCAAACCTGTGCTTATGGCTCTTGCGGAGGG
TCCTGGTGCTGAGGGTCCCCGCCTGGCCTCACCTAGTGGCAGCACCAGCTCAGGGCTGGA
GGTGGTGGCTCCTGAGGGTACCTCAGCCCCAGGTGGTGGCCCGGGAACCCTGGATGACAG
TGCCACCATTTGCCGTGTCTGCCAGAAGCCAGGCGATCTGGTTATGTGCAACCAGTGTGA
GTTTTGTTTCCACCTGGACTGTCACCTGCCGGCCCTGCAGGATGTACCAGGGGAGGAGTG
GAGCTGCTCACTCTGCCATGTGCTCCCTGACCTGAAGGAGGAGGATGGCAGCCTCAGCCT
GGATGGTGCAGACAGCACTGGCGTGGTGGCCAAGCTCTCACCAGCCAACCAGCGGAAATG
TGAGCGTGTACTGCTGGCCCTATTCTGTCACGAACCCTGCCGCCCCCTGCATCAGCTGGC
TACCGACTCCACCTTCTCCCTGGACCAGCCCGGTGGCACCCTGGATCTGACCCTGATCCG
TGCCCGCCTCCAGGAGAAGTTGTCACCTCCCTACAGCTCCCCACAGGAGTTTGCCCAGGA
TGTGGGCCGCATGTTCAAGCAATTCAACAAGTTAACTGAGGACAAGGCAGACGTGCAGTC
CATCATCGGCCTGCAGCGCTTCTTCGAGACGCGCATGAACGAGGCCTTCGGTGACACCAA
GTTCTCTGCTGTGCTGGTGGAGCCCCCGCCGATGAGCCTGCCTGGTGCTGGCCTGAGTTC
CCAGGAGCTGTCTGGTGGCCCTGGTGATGGCCCCTGAGGCTGGAGCCCCCATGGCCAGCC
CAGCCTGGCTCTGTTCTCTGTCCTGTCACCCCATCCCCACTCCCCTGGTGGCCTGACTCC
CACTCCCTGGTGGCCCCATCCCCCAGTTCCTCACGATATGGTTTTTACTTCTGTGGATTT
AATAAAAACTTCACCAGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_005762
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005762.2, NP_005753.1
RefSeq Size 2989 bp
RefSeq ORF 2508 bp
Locus ID 10155
UniProt ID Q13263
Cytogenetics 19q13.43
Domains BBC, BROMO, PHD, RING, zf-B_box
Protein Families Protein Kinase, Stem cell - Pluripotency, Transcription Factors
Summary The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. provided by RefSeq, Jul 2008
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Resources
"NM_005762" in other vectors (6)
SKU Description Size Price
RC201205 TRIM28 (Myc-DDK-tagged)-Human tripartite motif containing 28 (TRIM28) 10 ug
$765.00
RC201205L1 Lenti ORF clone of Human tripartite motif containing 28 (TRIM28), Myc-DDK-tagged 10 ug
$1,065.00
RC201205L2 Lenti ORF clone of Human tripartite motif containing 28 (TRIM28), mGFP tagged 10 ug
$1,065.00
RC201205L3 Lenti ORF clone of Human tripartite motif containing 28 (TRIM28), Myc-DDK-tagged 10 ug
$1,065.00
RC201205L4 Lenti ORF clone of Human tripartite motif containing 28 (TRIM28), mGFP tagged 10 ug
$1,065.00
RG201205 TRIM28 (tGFP-tagged) - Human tripartite motif containing 28 (TRIM28) 10 ug
$789.00 $965.00

file-alt Citations

KAP1-Mediated Epigenetic Suppression in Anti-RNA Viral Responses by Direct Targeting RIG-I and MDA5
Li, Qin, Wang et al
J Immunol (2021) 207 (7), 1903-1910 DOI: 10.4049/jimmunol.2100342
Cited by 14
AI Summary: OriGene contributed to this study by providing the KAP1 plasmid (SC321588) used to generate FLAG-KAP1 and HRP-conjugated secondary antibodies for detection assays.
Snippet: Anti-SETDB1 (11231-1-AP) used for the ChIP assay was purchased from ProteinTech. HRP-conjugated secondary Abs were purchased from OriGene KAP1 plasmid was purchased from OriGene (SC321588) and subcloned into FLAG tag to generate FLAG-KAP1
TRIM28 SUMOylates and stabilizes NLRP3 to facilitate inflammasome activation
Qin, Li, Liang et al
Nat Commun (2021) 12 (1), 4794 DOI: 10.1038/s41467-021-25033-4
Cited by 167
AI Summary: In this study, OriGene’s anti-Myc antibody (catalog number N7143) was used to detect Myc-tagged proteins, aiding in the investigation of TRIM28-mediated SUMOylation and stabilization of NLRP3 during inflammasome activation.
Snippet: d anti-β-actin (66009-1-Ig, 1:2000) were from Proteintech;Alexa Fluor 633 (A-21071, 1:500) and Alexa Fluor 488 (A-11059, 1:500) were from Thermo Fisher Scientific;anti-Myc (N7143, 1:1000) was from ORIGENE. Anti-Flag and anti-Myc are the same isotypes. Cell culture To obtain mouse primary PMs, C57BL/6J mice (male, 6–8 weeks old) were injected i.p. with 3% Brewer’s thiogly-collate broth. Thr
FigureFigureFigure
WB
ZNF764 haploinsufficiency may explain partial glucocorticoid, androgen, and thyroid hormone resistance associated with 16p11.2 microdeletion
Kino, Pavlatou, Moraitis et al
J. Clin. Endocrinol. Metab. (2012) 97 (8), E1557-66 DOI: 10.1210/jc.2011-3493
Cited by 17
AI Summary: OriGene contributed to this study by providing the pCMV6-SPORT-TRIM28 plasmid, which was used to investigate the role of TRIM28 (TIF1β) in nuclear hormone receptor transcriptional activity related to ZNF764 haploinsufficiency.
Snippet: A–C, The patient has short stature and facial and genital abnormalities. ... pCMV6-SPORT-TRIM28 (for human TIF1β), pCRE-Luc, and pGL4.73[hRluc/SV40] were purchased from OriGene (Rockville, MD), Agilent Technologies, Inc. (Santa Clara, CA), and Promega Corp.
Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 ligase and negative regulator of IFN regulatory factor 7
Liang, Deng, Li et al
J. Immunol. (2011) 187 (9), 4754-63 DOI: 10.4049/jimmunol.1101704
Cited by 184
AI Summary: OriGene's human full-length TRIM28 product was used in this study to investigate and demonstrate TRIM28's role as a SUMO E3 ligase and negative regulator of IRF7.
Snippet: TAG3 (Stratagene). We purchased human full-length TRIM28 from Origene and subcloned it into pKH3 vector to generate pKH3-TRIM28. DNA fragments ... sample buffer. In vitro translation and GST pull down. Methionine-labeled
Interactions of ErbB4 and Kap1 connect the growth factor and DNA damage response pathways
Gilmore-Hebert, Ramabhadran, Stern
Mol Cancer Res (2010) 8 (10), 1388-98 DOI: 10.1158/1541-7786.MCR-10-0042
AI Summary: In this study, OriGene products—including the ERBB4 expression plasmid (RC200003) and ERBB4 shRNA (TG311019)—were used to manipulate ERBB4 levels in cells, enabling the authors to investigate the molecular interactions and functional consequences of ErbB4 and Kap1 in linking growth factor and DNA damage response pathways.
FigureFigure
Showing 1-5 of 5 papers.
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