NGLY1 (NM_018297) Human Untagged Clone
$914.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | NGLY1 |
| Synonyms | CDDG; CDG1V; PNG-1; PNG1; PNGase |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_018297 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_018297.2, NP_060767.2 |
| RefSeq Size | 2182 bp |
| RefSeq ORF | 1965 bp |
| Locus ID | 55768 |
| UniProt ID | Q96IV0 |
| Cytogenetics | 3p24.2 |
| Domains | PAW, PUG, TGc |
| Summary | This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Feb 2009 Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_018297" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC203447 | NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 | 10 ug |
$914.00
|
|
| RC203447L1 | Lenti-ORF clone of NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 | 10 ug |
$1,214.00
|
|
| RC203447L2 | Lenti-ORF clone of NGLY1 (mGFP-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 | 10 ug |
$1,214.00
|
|
| RC203447L3 | Lenti-ORF clone of NGLY1 (Myc-DDK-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 | 10 ug |
$1,214.00
|
|
| RC203447L4 | Lenti-ORF clone of NGLY1 (mGFP-tagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 | 10 ug |
$1,214.00
|
|
| RG203447 | NGLY1 (tGFP-tagged) - Human N-glycanase 1 (NGLY1), transcript variant 1 | 10 ug |
$1,114.00
|
|
| SC113603 | NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 1 | 10 ug |
$975.00
|
|
Citations
Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation
Galeone, Adams, Matsuda et al
Elife (2020) 9 DOI: 10.7554/eLife.55596
Galeone, Adams, Matsuda et al
Elife (2020) 9 DOI: 10.7554/eLife.55596
AI Summary: OriGene’s human NGLY1 cDNA clone (SC320763 in pCMV6-AC vector) was used as a template to generate mutated NGLY1 transgenes for functional studies in this paper.
Snippet: p_ exon (Figure 1B [/pmc/articles/PMC7394544/figure/fig1/], wild-type and 3NQ). GENERATION OF _NGLY1_ OVEREXPRESSION _DROSOPHILA_ TRANSGENES Human _NGLY1_ cDNA in _pCMV6-AC_ vector (clone SC320763, OriGene) was used as a template for site-directed mutagenesis to introduce the c.121-122AA > CC and c.236-241GCCTTT > GCCGCT mutations, which result in the generation of _NGLY1N41P_ and _NGLY1G79A
Tissue-specific regulation of BMP signaling by Drosophila N-glycanase 1
Galeone, Han, Huang et al
Elife (2017) 6 DOI: 10.7554/eLife.27612
Galeone, Han, Huang et al
Elife (2017) 6 DOI: 10.7554/eLife.27612
AI Summary: OriGene’s Human NGLY1 cDNA in pCMV6-AC vector (clone SC320763) was used as a template for site-directed mutagenesis to generate a clinically relevant NGLY1 mutant, enabling the study of its functional impact in this research.
Snippet: Human NGLY1 cDNA in pCMV6-AC vector (clone SC320763, OriGene) was used as template for site-directed mutagenesis to introduce the c.1205_1207del clinical mutation (Enns et al., 2014), which results in the generation of NGLY1-ΔR402
Showing 1-2 of 2 papers.
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