NTAN1 (NM_173474) Human Untagged Clone

SKU
SC319519
NTAN1 (untagged)-Human N-terminal asparagine amidase (NTAN1)
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NTAN1
Synonyms PNAA; PNAD
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_173474.2 CCGCGATGCCGCTGCTCGTCGAGGGGCGGCGAGTGCGGCTGCCGCAGTCAGCCGGGGACC
TCGTCCGAGCCCACCCGCCTTTGGAGGAAAGAGCCAGACTTCTCAGAGGTCAGTCTGTTC
AACAAGTGGGACCCCAGGGCCTTCTGTATGTTCAGCAAAGAGAGCTTGCAGTGACCTCCC
CAAAGGATGGCTCCATCTCCATTCTGGGTTCTGATGATGCCACTACTTGTCACATTGTGG
TCCTGAGGCACACAGGTAATGGGGCCACCTGCTTGACACATTGTGACGGAACCGACACCA
AAGCTGAGGTCCCCTTGATCATGAACTCCATAAAATCCTTTTCTGACCACGCTCAATGTG
GAAGGCTGGAAGTACACCTTGTTGGAGGCTTCAGTGACGACAGGCAGTTGTCACAAAAAC
TCACTCATCAACTTCTTAGTGAATTTGACAGGCAAGAAGATGACATTCACTTAGTGACAT
TATGTGTGACAGAATTAAATGACCGGGAAGAAAACGAAAACCACTTTCCAGTAATATATG
GCATTGCTGTCAACATTAAGACTGCAGAGATTTACAGAGCATCCTTTCAAGATCGGGGTC
CGGAGGAGCAGCTTCGTGCTGCGCGAACTTTAGCAGGAGGACCAATGATTAGCATTTATG
ATGCAGAGACAGAACAACTTCGTATAGGACCGTACTCCTGGACACCATTTCCACATGTGG
ATTTCTGGTTGCACCAAGATGACAAGCAAATACTAGAGAATCTTTCCACTTCGCCTCTGG
CTGAGCCACCCCACTTTGTTGAACATATTAGATCTACCTTGATGTTTTTAAAAAAACACC
CATCTCCAGCTCACACACTGTTTTCTGGAAATAAAGCCCTACTCTACAAAAAAAATGAAG
ATGGCTTGTGGGAAAAGATCTCTTCTCCAGGAAGTTAAAAAACATGAATTACCAAAGAAA
GCACCTTCTTGGCCTGACAGACCATTGGTGGGGCTGGCACGAATCCAGATCTGGATCCTA
CATCTGTTGGGTCTTAGGCCTCCTTCCCTCCTCAGTGTCTTTCAAATGACTTTCATCAAA
TGACTTTCAAAATAAAACCTTATTTTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_173474
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173474.2, NP_775745.1
RefSeq Size 1137 bp
RefSeq ORF 933 bp
Locus ID 123803
UniProt ID Q96AB6
Cytogenetics 16p13.11
Summary The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms. provided by RefSeq, Jul 2012
Transcript Variant: This variant (1) represents the longesr transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_173474" in other vectors (4)
SKU Description Size Price
RC205697 NTAN1 (Myc-DDK-tagged)-Human N-terminal asparagine amidase (NTAN1) 10 ug
$300.00
RC205697L3 Lenti ORF clone of Human N-terminal asparagine amidase (NTAN1), Myc-DDK-tagged 10 ug
$600.00
RC205697L4 Lenti ORF clone of Human N-terminal asparagine amidase (NTAN1), mGFP tagged 10 ug
$600.00
RG205697 NTAN1 (tGFP-tagged) - Human N-terminal asparagine amidase (NTAN1) 10 ug
$489.00 $500.00

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