NDUFAF7 (NM_144736) Human Untagged Clone

SKU SC319491

NDUFAF7 (untagged)-Human chromosome 2 open reading frame 56 (C2orf56), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: NDUFAF7
• Synonyms: C2orf56; MidA; PRO1853
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_144736.3 CCCAGCTCCTGGCGGAGCGAGCTAGCCTGCGAATTTCAGCATGAGTGTACTGCTGAGGTC; AGGTTTGGGGCCGTTGTGTGCCGTGGCGCGCGCAGCCATTCCTTTTATTTGGAGAGGGAA; ATACTTCAGCTCCGGGAATGAGCCTGCAGAAAACCCGGTGACGCCGATGCTGCGGCATCT; TATGTACAAAATAAAGTCTACTGGTCCCATCACTGTGGCCGAGTACATGAAGGAGGTGTT; GACTAATCCAGCCAAGGGTTATTATGTGTACCGTGACATGCTAGGCGAAAAAGGAGATTT; CATTACTTCACCTGAAATAAGTCAAATCTTTGGGGAGCTACTAGGTATATGGTTCATTAG; TGAATGGATGGCCACTGGAAAAAGCACAGCTTTCCAGCTGGTGGAACTGGGCCCAGGTAG; GGGAACCCTCGTGGGAGATATTTTGAGGGTGTTCACTCAACTTGGATCTGTGCTGAAAAA; TTGTGACATTTCAGTACATCTGGTAGAGGTAAGCCAAAAATTAAGTGAGATTCAAGCATT; GACACTGACTAAAGAGAAGGTCCCGTTAGAGCGAAATGCTGGATCCCCAGTGTATATGAA; AGGTGTCACTAAGTCTGGGATTCCAATTTCCTGGTACCGAGATCTGCACGATGTTCCAAA; AGGGTACAGCTTTTATCTTGCACATGAATTTTTTGATGTTCTTCCTGTGCATAAATTTCA; GAAAACACCACAGGGATGGCGAGAAGTATTTGTTGACATTGATCCACAGGTTTCTGATAA; ACTGAGGTTTGTTTTGGCACCTTCTGCCACCCCAGCAGAAGCCTTCATACAACATGACGA; AACAAGGGATCATGTTGAAGTGTGTCCTGATGCTGGTGTTATCATCGAGGAACTTTCTCA; ACGCATTGCATTAACTGGAGGTGCTGCACTGGTTGCTGATTATGGTCATGATGGAACAAA; GACAGATACCTTCAGAGGGTTTTGCGACCACAAGCTTCATGATGTCTTAATTGCCCCAGG; AACAGCAGATCTAACAGCTGATGTGGACTTCAGTTATTTGCGAAGAATGGCACAGGGAAA; AGTAGCCTCTCTGGGCCCAATAAAACAACACACATTTTTAAAAAATATGGGTATTGATGT; CCGGCTGAAGGTTCTTTTAGATAAATCAAATGAGCCATCAGTGAGGCAGCAGTTACTTCA; AGGATATGATATGTTAATGAATCCAAAGAAGATGGGAGAGAGATTTAACTTTTTTGCCTT; GCTACCTCATCAGAGACTTCAAGGTGGAAGATATCAGAGGAATGCACGTCAGTCAAAACC; CTTTGCATCCGTTGTAGCTGGGTTTAGTGAACTTGCTTGGCAGTGATATTTCAGCTTGGA; CATTTTACCCTTCAGTCGGCCCAAGAAATCAAAATAAAGGAAACACATTTCATATAAAAA; AAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_144736
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_144736.3, NP_653337.1
• RefSeq Size: 2205 bp
• RefSeq ORF: 1326 bp
• Locus ID: 55471
• UniProt ID: Q7L592
• Cytogenetics: 2p22.2
• Domains: DUF185
• Summary: This gene encodes an assembly factor protein which helps in the assembly and stabilization of Complex I, a large multi-subunit enzyme in the mitochondrial respiratory chain. Complex I is involved in several physiological activities in the cell, including metabolite transport and ATP synthesis. The encoded protein is a methyltransferase which methylates Arg85 of a subunit of Complex I in the early stages of its assembly. A pseudogene related to this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).