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CENPN (NM_018455) Human Untagged Clone

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SKU
SC319184
CENPN (untagged)-Human centromere protein N (CENPN), transcript variant 3
  $300.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol CENPN
Synonyms BM039; C16orf60; CENP-N; ICEN32
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_018455.3 CACCTCGCTCGCAGCCTCCCCAGCGCAGCAGCCCGGCTGTGGGCCTGCGGCAGCCGGGTC
TTCCTGGTCCCCACCTCCTGGGGCCGACGGGCGGCAGGAAGGGGCTCGGCGGGACGCGCC
GTCAGGGACCTGAGGAGGAACAACGGAACGCGTTCGGAACGGCCTGGACTCCCGAGACTC
ACCCGACTCGTGGCCACACCGGGAGAACTGAAGCGGCAGTAGCCGGCGGAGACGCCCGAC
CCGAAGGCCGGCTGCTAGGGAGCAGACAGCTGAACCGCTTGCCAGACGCCGAAACCCAGT
GACGCCCTCCACCGCTCCACCGTGCTCCCGGCTCCCCGCCCCCGCCGCCCGCGGGCCCCA
AGGCGCATGCGCCGCCTGTCCTGGAGGGGCCCATTTCCGTCCGTCGTGGGGGGAGGCACA
GTGAGTCCACTGGGGCACGGCAGCGTCTAAGCCACAAGCCGAGCACATAAGCCAGGTCCT
AACGGAGCCTATGTGTAAGTCCACTACTGGTGCAAGGTTGCACACTTCTAAGAAGAGCGG
CGTGGGGGGCTCGGCGACCTTCGCTTCAGTCGCTCCCCCGTGCAGTCCCCTGTGCCCAAG
ACACAGCCTGATGCTTGTGCTCCGGTGGGCGGAGCTTGGAGGCGGCGGGAACTGCAATTG
GTGGCTTTGAAGGCGCGGCGAGCGGGAACAGCTCTTGAGGAGTGAGACTGCAGGAGATGT
GGGCCGTGCCAAAGAGATGGATGAGACTGTTGCTGAGTTCATCAAGAGGACCATCTTGAA
AATCCCCATGAATGAACTGACAACAATCCTGAAGGCCTGGGATTTTTTGTCTGAAAATCA
ACTGCAGACTGTAAATTTCCGACAGAGAAAGGAATCTGTAGTTCAGCACTTGATCCATCT
GTGTGAGGAAAAGCGTGCAAGTATCAGTGATGCTGCCCTGTTAGACATCATTTATATGCA
ATTTCATCAGCACCAGAAAGTTTGGGATGTTTTTCAGATGAGTAAAGGACCAGGTGAAGA
TGTTGACCTTTTTGATATGAAACAATTTAAAAATTCGTTCAAGAAAATTCTTCAGAGAGC
ATTAAAAAATGTGACAGTCAGCTTCAGAGAAACTGAGGAGAATGCAGTCTGGATTCGAAT
TGCCTGGGGAACACAGTACACAAAGCCAAACCAGTACAAACCTACCTACGTGGTGTACTA
CTCCCAGACTCCGTACGCCTTCACGTCCTCCTCCATGCTGAGGCGCAATACACCGCTTCT
GGGTCAGGAGTTAGAAGCTACTGGGAAAATCTACCTCCGACAAGAGGAGATCATTTTAGA
TATTACCGAAATGAAGAAAGCTTGCAATTAGTGAACATGAAAGGAAAATAAAAATTCCTC
ACAGTCAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire |
ACCN NM_018455
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018455.3, NP_060925.1
RefSeq Size 1405 bp
RefSeq ORF 615 bp
Locus ID 55839
UniProt ID Q96H22
Cytogenetics 16q23.2
Protein Families Druggable Genome
Summary The protein encoded by this gene forms part of the nucleosome-associated complex and is important for kinetochore assembly. It is bound to kinetochores during S phase and G2 and recruits other proteins to the centromere. Pseudogenes of this gene are located on chromosome 2. Alternative splicing results in multiple transcript variants that encode different protein isoforms. provided by RefSeq, Jul 2012
Transcript Variant: This variant (3) lacks several exons in the 3' coding region and uses an alternate 3' terminal exon, compared to variant 1. The resulting protein (isoform 3) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_018455" in other vectors (4)
SKU Description Size Price
RC203426 CENPN (Myc-DDK-tagged)-Human centromere protein N (CENPN), transcript variant 3 10 ug
$300.00
RC203426L3 Lenti ORF clone of Human centromere protein N (CENPN), transcript variant 3, Myc-DDK-tagged 10 ug
$600.00
RC203426L4 Lenti ORF clone of Human centromere protein N (CENPN), transcript variant 3, mGFP tagged 10 ug
$600.00
RG203426 CENPN (tGFP-tagged) - Human centromere protein N (CENPN), transcript variant 3 10 ug
$489.00 $500.00

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