Importin4 (IPO4) (NM_024658) Human Untagged Clone

SKU SC318486

IPO4 (untagged)-Human importin 4 (IPO4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Importin4
• Synonyms: Imp4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318486 representing NM_024658.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGAGTCAGCCGGGCTAGAGCAGCTCCTACGGGAGCTGCTGCTACCGGACACCGAGCGCATCCGTCGG; GCCACGGAACAGCTCCAGATCGTTCTTCGGGCCCCCGCCGCTTTGCCGGCTCTCTGCGACCTGCTAGCC; TCGGCGGCCGACCCCCAGATCCGCCAGTTTGCGGCCGTGCTGACCCGCAGACGACTGAACACCCGCTGG; CGACGGCTGGCGGCGGAGCAACGGGAGAGCCTCAAGTCCCTGATCCTGACGGCCCTGCAGAGAGAAACA; GAGCACTGTGTGAGCCTCAGCCTGGCCCAGCTCTCAGCCACCATTTTTCGAAAGGAAGGCTTGGAGGCC; TGGCCACAGCTTTTGCAGCTGCTTCAGCACAGTACCCACAGCCCCCACAGCCCAGAGAGAGAGATGGGG; CTTTTGCTGCTAAGTGTGGTGGTGACCTCCCGGCCCGAGGCCTTCCAACCCCACCACCGGGAGCTTCTT; CGGCTTCTGAATGAGACTCTTGGTGAGGTGGGCTCTCCTGGGCTGCTCTTCTACTCCCTGCGCACTCTG; ACCACCATGGCTCCCTACCTCAGCACTGAAGATGTGCCTCTCGCTCGGATGTTGGTGCCCAAGCTGATC; ATGGCCATGCAGACTCTGATCCCCATAGATGAGGCAAAGGCCTGTGAGGCCCTTGAGGCTTTGGATGAA; CTGTTGGAGTCAGAGGTGCCGGTCATCACCCCCTACCTCTCTGAAGTCCTCACATTCTGCCTGGAGGTA; GCTAGAAATGTGGCCCTGGGCAATGCGATACGCATACGTATTCTCTGCTGCCTCACTTTCTTGGTCAAA; GTCAAGAGCAAGGCCTTACTGAAGAATCGTCTCCTGCCACCCTTGCTGCACACCCTTTTCCCCATTGTG; GCTGCTGAGCCCCCACCAGGCCAGTTGGATCCCGAGGACCAGGATTCAGAAGAGGAAGAGTTGGAGATT; GAGCTGATGGGGGAGACTCCCAAGCATTTCGCTGTACAAGTTGTGGACATGCTGGCACTACACCTGCCC; CCCGAGAAGCTCTGTCCCCAGCTGATGCCCATGTTGGAAGAGGCTTTGCGGAGCGAGAGCCCATACCAG; CGCAAAGCTGGACTCCTGGTGCTGGCCGTGCTGTCTGACGGAGCTGGCGACCACATCAGGCAGAGACTG; CTGCCCCCACTGCTGCAGATTGTGTGCAAGGGCCTGGAGGACCCCTCGCAAGTTGTACGCAATGCTGCG; CTGTTTGCCCTGGGCCAGTTCTCAGAAAACCTACAGCCCCATATCAGCAGCTATTCAAGGGAGGTAATG; CCACTGCTCCTCGCCTACTTGAAGTCGGTGCCTCTTGGACACACACACCACCTAGCCAAGGCCTGCTAT; GCCCTGGAGAATTTTGTGGAGAACCTAGGGCCCAAGGTGCAGCCCTACCTTCCGGAGCTTATGGAATGC; ATGCTGCAGCTTCTGAGGAACCCCAGCAGTCCCCGGGCCAAGGAGCTGGCTGTGAGCGCCCTGGGAGCC; ATTGCTACGGCTGCCCAGGCCTCGCTGCTGCCCTACTTCCCTGCCATCATGGAGCACCTGCGGGAATTC; CTGTTAACAGGCCGTGAGGACCTTCAGCCTGTGCAGATCCAGAGCCTGGAGACACTGGGGGTGCTGGCA; CGAGCAGTGGGGGAGCCCATGAGGCCGCTGGCTGAGGAATGCTGCCAGCTGGGTCTGGGCCTCTGCGAC; CAGGTAGACGACCCTGACTTGCGGCGCTGCACGTACAGCCTATTTGCAGCCTTATCGGGTCTGATGGGT; GAGGGCCTGGCGCCCCACTTGGAACAGATCACCACGCTCATGCTGCTGTCACTGCGTTCCACCGAGGGC; ATTGTGCCTCAGTATGACGGGAGCAGCTCCTTCCTTCTGTTTGACGATGAGAGTGATGGGGAAGAAGAG; GAGGAGCTCATGGATGAGGATGTGGAAGAAGAGGATGACTCAGAGATCTCAGGGTACAGCGTGGAGAAT; GCCTTCTTCGATGAGAAGGAAGACACCTGTGCTGCCGTGGGGGAGATCTCTGTGAACACCAGTGTGGCC; TTCCTTCCATACATGGAAAGTGTCTTTGAAGAAGTATTTAAACTGCTGGAGTGCCCTCACCTGAATGTG; CGGAAGGCAGCCCATGAGGCTCTGGGTCAGTTTTGCTGTGCACTGCACAAGGCCTGTCAAAGCTGCCCC; TCGGAACCCAACACTGCTGCTTTGCAGGCTGCCCTGGCCCGAGTCGTGCCATCCTACATGCAGGCAGTG; AACAGGGAGCGGGAACGCCAGGTGGTGATGGCCGTGCTGGAGGCCCTGACAGGGGTGCTCCGCAGCTGT; GGGACCCTCACACTGAAGCCCCCTGGGCGCCTCGCTGAGCTCTGTGGCGTGCTCAAGGCTGTGCTGCAG; AGGAAGACAGCCTGTCAGGATACTGACGAGGAGGAGGAAGAGGAAGATGATGATCAGGCTGAATACGAC; GCCATGTTGCTGGAGCACGCTGGAGAGGCCATCCCTGCCCTGGCAGCCGCGGCTGGGGGAGACTCCTTT; GCCCCATTCTTTGCCGGTTTCCTGCCATTATTGGTGTGCAAGACAAAACAGGGCTGCACAGTGGCAGAG; AAGTCCTTTGCAGTGGGGACCTTGGCAGAGACTATTCAGGGCCTGGGTGCTGCCTCAGCCCAGTTTGTG; TCTCGGCTGCTCCCTGTGCTGTTGAGCACCGCCCAAGAGGCAGACCCCGAGGTGCGAAGCAATGCCATC; TTCGGGATGGGCGTGCTGGCAGAGCATGGGGGCCACCCTGCCCAGGAACACTTCCCCAAGCTGCTGGGG; CTCCTTTTTCCCCTCCTGGCGCGGGAGCGACATGATCGTGTCCGTGACAACATCTGTGGGGCACTTGCC; CGCCTGTTGATGGCCAGTCCCACCAGGAAACCAGAGCCCCAGGTGCTGGCTGCCCTACTGCATGCCCTG; CCACTGAAGGAGGACTTGGAGGAGTGGGTCACCATTGGGCGCCTCTTCAGCTTCCTGTACCAGAGCAGC; CCTGACCAGGTTATAGATGTGGCTCCCGAGCTTCTGCGTATCTGCAGCCTCATTCTGGCTGACAACAAG; ATCCCACCAGACACCAAGGCCGCACTGTTGCTGCTCCTGACGTTCCTGGCCAAACAGCACACCGACAGC; TTTCAAGCAGCTCTGGGCTCACTGCCTGTTGACAAGGCTCAGGAGCTCCAGGCTGTACTGGGCCTCTCC; TAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_024658
• Insert Size: 3246 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_024658.3
• RefSeq Size: 3606 bp
• RefSeq ORF: 3246 bp
• Locus ID: 79711
• UniProt ID: Q8TEX9
• Cytogenetics: 14q12
• Domains: HEAT_PBS, IBN_NT
• Protein Families: Druggable Genome
• MW: 118.7 kDa
• Summary: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the shorter transcript.