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USP53 (NM_019050) Human Untagged Clone

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SKU
SC318481
USP53 (untagged)-Human ubiquitin specific peptidase 53 (USP53)
  $935.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol USP53
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC318481 representing NM_019050.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCATGGGTAAAATTCTTACGGAAACCTGGTGGCAATCTTGGAAAAGTTTATCAGCCTGGAAGTATG
CTATCACTAGCCCCTACCAAAGGCTTGTTAAATGAACCAGGACAAAACAGCTGCTTTCTTAATAGCGCT
GTACAGGTTTTATGGCAATTGGATATATTCCGACGAAGCTTGCGGGTTTTGACTGGACATGTTTGTCAG
GGAGATGCCTGTATATTTTGTGCATTGAAGACGATATTTGCACAGTTCCAACACAGTCGAGAAAAAGCA
CTTCCCTCAGATAACATAAGGCATGCTCTTGCAGAAAGTTTCAAAGATGAGCAGCGATTTCAACTTGGC
CTTATGGATGATGCTGCGGAGTGCTTTGAAAATATGTTGGAGAGGATTCATTTTCACATAGTGCCAAGC
AGAGATGCAGACATGTGTACCTCTAAATCTTGTATCACTCACCAGAAGTTTGCTATGACTCTGTATGAA
CAGTGTGTGTGTCGTAGCTGTGGAGCATCGTCAGATCCTCTACCTTTTACAGAATTTGTGCGGTACATT
TCTACAACAGCCTTATGCAATGAGGTTGAAAGAATGTTGGAAAGGCATGAACGCTTTAAACCTGAAATG
TTTGCAGAATTGCTACAAGCAGCAAATACAACAGATGACTATAGGAAATGTCCTAGTAACTGTGGCCAA
AAAATAAAAATTCGCCGTGTTTTAATGAATTGCCCAGAGATTGTTACAATTGGTTTAGTCTGGGACTCC
GAGCATTCTGACTTGACCGAAGCTGTTGTTCGGAATCTAGCAACACATCTTTATCTTCCTGGGCTTTTT
TATAGAGTTACTGATGAAAATGCCAAAAATAGTGAACTTAACCTTGTTGGTATGATCTGCTACACCAGC
CAACATTATTGTGCCTTTGCATTTCACACCAAAAGTTCCAAATGGGTATTTTTTGATGATGCAAATGTG
AAAGAGATTGGAACTAGATGGAAAGATGTTGTCTCCAAATGCATTCGATGCCACTTTCAGCCACTACTT
TTGTTTTATGCAAACCCAGATGGCACAGCAGTTTCTACTGAGGATGCACTCAGGCAGGTCATCAGCTGG
TCACATTACAAATCTGTTGCAGAAAATATGGGATGTGAAAAGCCTGTAATTCATAAGTCAGATAATTTA
AAAGAAAATGGATTTGGTGATCAGGCAAAGCAGAGAGAAAATCAGAAATTTCCAACTGATAATATTTCA
TCATCTAATCGGAGCCACAGTCACACAGGTGTAGGGAAAGGACCAGCTAAGTTAAGTCACATTGATCAA
AGGGAAAAGATAAAAGACATTTCCAGAGAATGTGCTCTGAAAGCTATTGAACAGAAAAACTTACTTTCT
TCACAAAGGAAAGATTTAGAGAAGGGACAAAGAAAAGATTTAGGACGACATAGAGATTTGGTTGATGAA
GACCTTTCACATTTCCAATCTGGATCACCTCCTGCCCCAAATGGTTTTAAACAACATGGGAATCCACAT
CTATATCATAGTCAAGGAAAAGGATCATATAAACATGACCGAGTTGTACCTCAGAGTCGAGCTTCTGCA
CAAATAATAAGTTCAAGTAAATCCCAGATTCTTGCTCCAGGAGAGAAAATAACTGGCAAAGTTAAGAGT
GACAATGGCACTGGATATGACACAGACAGCAGCCAAGATTCTAGGGATAGAGGAAACAGCTGTGATAGC
AGCAGTAAAAGCCGGAACCGAGGTTGGAAACCTATGAGAGAAACATTAAATGTTGATAGTATTTTTAGT
GAAAGTGAAAAAAGACAGCATAGTCCAAGACATAAACCAAATATCAGTAATAAGCCTAAATCTAGCAAG
GATCCGAGTTTTAGTAATTGGCCAAAAGAGAATCCAAAGCAAAAAGGTTTAATGACCATATATGAAGAT
GAAATGAAGCAGGAAATAGGAAGCAGAAGTTCCCTTGAATCTAATGGAAAAGGAGCAGAGAAAAATAAA
GGCCTTGTAGAGGGTAAAGTGCATGGTGATAATTGGCAGATGCAAAGGACTGAGTCTGGATATGAAAGC
AGTGATCACATCAGTAATGGTTCTACTAATTTGGACTCACCTGTTATCGATGGAAATGGTACAGTAATG
GATATCAGTGGTGTTAAAGAAACAGTATGCTTCAGTGACCAGATTACGACAAGCAACCTAAATAAAGAA
CGTGGGGACTGTACCTCCCTTCAGAGCCAACATCACTTAGAAGGCTTTAGAAAAGAACTCAGGAATTTG
GAAGCAGGCTATAAATCTCATGAATTCCACCCAGAATCACATTTACAAATAAAAAATCATTTGATAAAA
AGATCACATGTACATGAAGACAATGGAAAGTTATTTCCTTCATCCAGTCTACAAATACCCAAGGACCAT
AATGCAAGAGAACATATCCACCAGTCAGATGAACAGAAACTTGAAAAACCGAATGAATGCAAATTTTCT
GAGTGGCTTAATATAGAAAATTCTGAGAGAACAGGTTTGCCTTTTCACGTTGATAACTCTGCTTCTGGG
AAGAGAGTGAACAGTAATGAACCATCTTCATTATGGTCTTCACACCTAAGAACTGTTGGGTTAAAGCCA
GAAACTGCTCCTCTCATCCAGCAACAAAATATCATGGATCAATGTTACTTTGAGAACTCTCTATCCACA
GAATGTATAATTCGGTCAGCCAGCAGATCTGATGGGTGTCAGATGCCAAAACTTTTTTGCCAGAATCTA
CCACCCCCTTTGCCACCAAAGAAATATGCTATAACCAGTGTGCCACAGTCAGAGAAAAGCGAATCTACA
CCTGATGTCAAACTTACAGAGGTGTTTAAAGCTACCTCTCATCTTCCGAAGCACAGTTTAAGTACAGCT
TCAGAACCAAGTTTAGAAGTGAGTACACATATGAATGATGAAAGACATAAAGAAACATTTCAAGTGAGA
GAATGTTTTGGCAACACACCAAACTGTCCATCCAGCTCCTCAACTAACGATTTTCAGGCAAACTCAGGT
GCCATTGATGCATTTTGCCAACCAGAACTAGACTCTATTTCTACCTGTCCAAATGAGACAGTTTCATTA
ACTACCTATTTTTCAGTTGATAGCTGCATGACGGATACATATAGATTGAAATACCATCAGAGGCCCAAG
CTCTCTTTTCCAGAGAGCAGTGGCTTTTGTAATAATTCACTATCTTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_019050
Insert Size 3222 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019050.2
RefSeq Size 6612 bp
RefSeq ORF 3222 bp
Locus ID 54532
UniProt ID Q70EK8
Cytogenetics 4q26
MW 120.8 kDa
Summary Tight junction-associated protein that is involved in the survival of auditory hair cells and hearing. Maybe by modulating the barrier properties and mechanical stability of tight junctions (By similarity). Has no peptidase activity (PubMed:14715245).UniProtKB/Swiss-Prot Function
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"NM_019050" in other vectors (6)
SKU Description Size Price
RC223970 USP53 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 53 (USP53) 10 ug
$934.00
RC223970L1 Lenti ORF clone of Human ubiquitin specific peptidase 53 (USP53), Myc-DDK-tagged 10 ug
$1,234.00
RC223970L2 Lenti ORF clone of Human ubiquitin specific peptidase 53 (USP53), mGFP tagged 10 ug
$1,234.00
RC223970L3 Lenti ORF clone of Human ubiquitin specific peptidase 53 (USP53), Myc-DDK-tagged 10 ug
$1,234.00
RC223970L4 Lenti ORF clone of Human ubiquitin specific peptidase 53 (USP53), mGFP tagged 10 ug
$1,234.00
RG223970 USP53 (tGFP-tagged) - Human ubiquitin specific peptidase 53 (USP53) 10 ug
$1,134.00

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