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SPTLC3 (NM_018327) Human Untagged Clone

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SKU
SC317943
SPTLC3 (untagged)-Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3)
  $566.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol SPTLC3
Synonyms C20orf38; dJ718P11; dJ718P11.1; hLCB2b; LCB2B; LCB 3; LCB3; SPT 3; SPT3; SPTLC2L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC317943 representing NM_018327.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCTAACCCTGGAGGTGGTGCTGTTTGCAACGGGAAACTTCACAATCACAAGAAACAGAGCAATGGC
TCACAAAGCAGAAACTGCACAAAGAATGGAATAGTGAAGGAAGCCCAGCAAAATGGGAAGCCACATTTT
TATGATAAGCTCATTGTTGAATCGTTTGAGGAAGCACCCCTTCATGTTATGGTTTTCACTTACATGGGA
TATGGAATTGGAACCCTGTTTGGCTATCTCAGAGACTTTTTAAGAAACTGGGGAATAGAAAAATGCAAC
GCAGCTGTGGAACGAAAAGAACAAAAAGATTTTGTGCCACTGTATCAAGACTTTGAAAATTTTTATACA
AGAAACCTTTACATGCGAATCAGAGACAACTGGAACCGGCCCATCTGCAGTGCCCCAGGGCCTCTGTTT
GATTTGATGGAGAGGGTATCAGACGACTATAACTGGACGTTTAGGTTTACTGGAAGAGTCATCAAAGAT
GTCATCAACATGGGCTCCTATAACTTCCTTGGTCTTGCAGCCAAGTATGATGAGTCTATGAGGACAATA
AAGGATGTTTTAGAGGTGTATGGCACAGGCGTGGCCAGCACCAGGCATGAAATGGGCACCTTGGATAAG
CACAAGGAGTTGGAGGACCTTGTGGCTAAGTTCCTGAATGTGGAAGCAGCTATGGTCTTTGGGATGGGA
TTCGCAACTAACTCAATGAATATCCCAGCATTAGTTGGAAAGGGATGCCTCATTTTAAGTGATGAGTTA
AACCACACATCGCTTGTGCTTGGGGCCCGACTCTCAGGTGCAACCATAAGAATCTTCAAACACAACAAC
ACACAAAGCCTAGAGAAGCTCCTGAGAGATGCTGTCATCTATGGCCAGCCTCGAACCCGCAGAGCTTGG
AAAAAGATTCTCATCCTGGTGGAGGGTGTCTACAGCATGGAAGGTTCCATCGTGCATCTGCCCCAGATC
ATAGCTCTAAAGAAGAAATACAAGGCTTACCTCTACATAGATGAAGCTCACAGTATTGGGGCCGTGGGC
CCAACCGGCCGGGGTGTCACGGAGTTCTTTGGACTAGACCCTCATGAAGTTGATGTGCTCATGGGCACA
TTCACCAAAAGTTTTGGAGCTTCAGGAGGTTACATAGCTGGAAGGAAGGACCTCGTGGATTATTTACGG
GTTCACTCGCATAGTGCTGTTTATGCTTCATCCATGAGCCCACCGATAGCAGAGCAAATCATCAGATCA
CTAAAACTTATCATGGGACTGGATGGGACCACTCAAGGGCTGCAGAGAGTACAGCAACTTGCGAAAAAC
ACAAGATACTTCAGACAAAGACTGCAGGAAATGGGATTCATTATCTATGGCAATGAGAATGCTTCTGTT
GTTCCTCTGCTTCTTTATATGCCTGGTAAAGTAGCGGCTTTTGCAAGGCATATGCTAGAGAAAAAAATT
GGAGTGGTGGTCGTGGGATTTCCAGCCACTCCCCTCGCAGAAGCTCGGGCTCGGTTTTGTGTTTCAGCG
GCACATACCCGGGAGATGTTAGACACGGTTTTAGAAGCTCTTGATGAAATGGGTGATCTCTTGCAACTG
AAATATTCCCGGCACAAGAAGTCAGCACGTCCTGAGCTCTATGATGAGACGAGCTTTGAACTCGAAGAT
TAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_018327
Insert Size 1659 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018327.2
RefSeq Size 3855 bp
RefSeq ORF 1659 bp
Locus ID 55304
UniProt ID Q9NUV7
Cytogenetics 20p12.1
MW 62 kDa
Summary This gene encodes a subunit of the serine palmitoyltransferase complex which catalyzes the rate-limiting step in sphingolipid biosynthesis. This subunit metabolizes lauroyl- and myristoyl-CoA and generates C14 and C16-sphingoid bases. provided by RefSeq, Mar 2017
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"NM_018327" in other vectors (5)
SKU Description Size Price
RC224216 SPTLC3 (Myc-DDK-tagged)-Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3) 10 ug
$289.00 $514.00
RC224216L3 Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3), Myc-DDK-tagged 10 ug
$814.00
RC224216L4 Lenti ORF clone of Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3), mGFP tagged 10 ug
$814.00
RG224216 SPTLC3 (tGFP-tagged) - Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3) 10 ug
$489.00 $714.00
SC113546 SPTLC3 (untagged)-Human serine palmitoyltransferase, long chain base subunit 3 (SPTLC3) 10 ug
$1,118.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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