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USP5 (NM_001098536) Human Untagged Clone

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SKU
SC316428
USP5 (untagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1
  $1,258.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol USP5
Synonyms ISOT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC316428 representing NM_001098536.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGGAGCTGAGTGAGGAGGCGCTGCTGTCAGTATTACCGACGATCCGGGTCCCTAAGGCTGGAGAC
CGGGTCCACAAAGACGAGTGCGCCTTCTCCTTCGACACGCCGGAGTCTGAGGGGGGCCTCTACATCTGT
ATGAACACGTTTCTGGGCTTTGGGAAACAGTATGTGGAGAGACATTTCAATAAGACCGGCCAGCGAGTC
TACTTGCACCTCCGGCGGACCCGGCGCCCGAAAGAGGAGGACCCTGCTACAGGCACTGGAGACCCACCC
CGGAAGAAGCCCACGCGGCTGGCTATTGGTGTTGAAGGCGGATTTGACCTTAGCGAGGAGAAGTTTGAA
TTAGACGAGGATGTGAAGATTGTCATTTTGCCAGATTACCTGGAGATTGCCCGGGATGGACTGGGGGGA
CTGCCTGACATTGTCAGAGATCGGGTGACCAGTGCAGTGGAGGCCCTACTGTCGGCCGACTCAGCCTCC
CGCAAGCAGGAGGTGCAGGCATGGGATGGGGAAGTACGGCAGGTGTCTAAGCATGCCTTCAGCCTCAAG
CAGTTGGACAACCCTGCTCGAATCCCTCCCTGTGGCTGGAAGTGCTCCAAGTGTGACATGAGAGAGAAC
CTGTGGCTCAACCTGACTGATGGCTCCATCCTCTGTGGGCGACGCTACTTCGATGGCAGTGGGGGCAAC
AACCACGCTGTGGAGCACTACCGAGAGACAGGCTACCCGTTAGCTGTCAAGCTGGGCACCATCACCCCT
GATGGAGCTGACGTGTACTCATATGATGAGGATGACATGGTCCTGGACCCCAGCCTGGCTGAGCACCTG
TCCCACTTCGGCATCGACATGCTGAAGATGCAGAAGACAGACAAGACGATGACTGAGTTGGAGATAGAC
ATGAACCAGCGGATTGGTGAATGGGAGCTGATCCAGGAGTCAGGTGTGCCACTCAAGCCCCTGTTTGGG
CCTGGCTACACAGGCATCCGGAACCTGGGTAACAGCTGCTACCTCAACTCTGTGGTCCAGGTGCTCTTC
AGCATCCCTGACTTCCAGAGGAAGTATGTGGATAAGCTGGAGAAGATCTTCCAGAATGCCCCGACGGAC
CCTACCCAGGATTTCAGCACCCAGGTGGCCAAGCTGGGCCATGGCCTTCTCTCCGGGGAGTATTCCAAG
CCAGTACCGGAGTCGGGCGATGGGGAGCGGGTGCCAGAACAGAAGGAAGTTCAAGATGGCATTGCCCCT
CGGATGTTCAAGGCCCTCATCGGCAAGGGCCACCCTGAATTCTCCACCAACCGGCAGCAGGATGCCCAG
GAGTTCTTCCTTCACCTTATCAACATGGTGGAGAGGAATTGCCGGAGCTCTGAAAATCCTAATGAAGTG
TTCCGCTTCTTGGTGGAGGAAAAGATCAAGTGCCTGGCCACAGAGAAGGTGAAGTACACCCAGCGAGTT
GACTACATCATGCAGCTGCCTGTGCCCATGGATGCAGCCCTTAACAAAGAGGAGCTTCTGGAGTACGAG
GAGAAGAAGCGGCAAGCCGAAGAGGAGAAGATGGCACTGCCAGAACTGGTTCGGGCCCAGGTGCCCTTC
AGCTCTTGCCTGGAGGCCTACGGGGCCCCTGAGCAGGTCGATGACTTCTGGAGCACGGCCCTGCAGGCC
AAGTCAGTAGCTGTCAAGACCACACGATTTGCCTCATTCCCTGACTACCTGGTCATCCAGATCAAGAAG
TTCACCTTCGGCTTAGACTGGGTGCCCAAGAAACTGGATGTGTCCATCGAGATGCCAGAGGAGCTCGAC
ATCTCCCAGTTGAGGGGCACAGGGCTGCAGCCCGGAGAGGAGGAGCTGCCAGACATTGCCCCACCCCTG
GTCACTCCGGATGAGCCCAAAGGTAGCCTTGGTTTCTATGGCAACGAAGACGAAGACTCCTTCTGCTCC
CCTCACTTCTCCTCTCCGACATCGCCCATGCTGGATGAATCAGTCATCATCCAGCTGGTGGAGATGGGA
TTCCCTATGGACGCCTGCCGCAAAGCTGTCTACTACACGGGCAACAGCGGGGCTGAGGCCGCCATGAAC
TGGGTCATGTCACACATGGATGATCCAGATTTTGCAAACCCCCTCATCCTGCCTGGCTCTAGTGGGCCG
GGCTCCACAAGCGCAGCAGCCGACCCCCCTCCTGAGGACTGTGTGACCACCATTGTCTCCATGGGCTTC
TCCCGGGACCAGGCCTTGAAAGCGCTGCGGGCCACGAACAATAGTTTAGAACGGGCTGTGGACTGGATC
TTCAGTCACATTGACGACCTGGATGCTGAAGCTGCCATGGACATCTCAGAGGGCCGCTCAGCTGCCGAC
TCCATCTCTGAGTCTGTGCCAGTGGGACCTAAAGTCCGGGATGGTCCTGGAAAGTATCAGCTCTTTGCC
TTCATTAGTCACATGGGCACCTCTACCATGTGTGGTCACTACGTCTGCCACATCAAGAAAGAAGGCAGA
TGGGTGATCTACAATGACCAGAAAGTGTGTGCCTCCGAGAAGCCGCCCAAGGACCTGGGCTACATCTAC
TTCTACCAGAGAGTGGCCAGCTAA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_001098536
Insert Size 2577 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001098536.1
RefSeq Size 3214 bp
RefSeq ORF 2577 bp
Locus ID 8078
UniProt ID P45974
Cytogenetics 12p13.31
Protein Families Druggable Genome, Protease
MW 95.8 kDa
Summary Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 PubMed 7578059).supplied by OMIM, Mar 2010
Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).
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"NM_001098536" in other vectors (6)
SKU Description Size Price
RC224191 USP5 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1 10 ug
$1,178.00
RC224191L1 Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, Myc-DDK-tagged 10 ug
$1,478.00
RC224191L2 Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, mGFP tagged 10 ug
$1,478.00
RC224191L3 Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, Myc-DDK-tagged 10 ug
$1,478.00
RC224191L4 Lenti ORF clone of Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1, mGFP tagged 10 ug
$1,478.00
RG224191 USP5 (tGFP-tagged) - Human ubiquitin specific peptidase 5 (isopeptidase T) (USP5), transcript variant 1 10 ug
$1,378.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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