SSB (NM_003142) Human Untagged Clone

SKU SC316315

SSB (untagged)-Human Sjogren syndrome antigen B (autoantigen La) (SSB)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SSB
• Synonyms: La; La/SSB; LARP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003142 edited; CGTTGCTGTTGCTGTTTGTGAGCCTGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGAT; AGCCGCAATGGCTGAAAATGGTGATAATGAAAAGATGGCTGCCCTGGAGGCCAAAATCTG; TCATCAAATTGAGTATTATTTTGGCGACTTCAATTTGCCACGGGACAAGTTTCTAAAGGA; ACAGATAAAACTGGATGAAGGCTGGGTACCTTTGGAGATAATGATAAAATTCAACAGGTT; GAACCGTCTAACAACAGACTTTAATGTAATTGTGGAAGCATTGAGCAAATCCAAGGCAGA; ACTCATGGAAATCAGTGAAGATAAAACTAAAATCAGAAGGTCTCCAAGCAAACCCCTACC; TGAAGTGACTGATGAGTATAAAAATGATGTAAAAAACAGATCTGTTTATATTAAAGGCTT; CCCAACTGATGCAACTCTTGATGACATAAAAGAATGGTTAGAAGATAAAGGTCAAGTACT; AAATATTCAGATGAGAAGAACATTGCATAAAGCATTTAAGGGATCAATTTTTGTTGTGTT; TGATAGCATTGAATCTGCTAAGAAATTTGTAGAGACCCCTGGCCAGAAGTACAAAGAAAC; AGACCTGCTAATACTTTTCAAGGACGATTACTTTGCCAAAAAAAATGAAGAAAGAAAACA; AAATAAAGTGGAAGCTAAATTAAGAGCTAAACAGGAGCAAGAAGCAAAACAAAAGTTAGA; AGAAGATGCTGAAATGAAATCTCTAGAAGAAAAGATTGGATGCTTGCTGAAATTTTCGGG; TGATTTAGATGATCAGACCTGTAGAGAAGATTTACACATACTTTTCTCAAATCATGGTGA; AATAAAATGGATAGACTTCGTCAGAGGAGCAAAAGAGGGGATAATTCTATTTAAAGAAAA; AGCCAAGGAAGCATTGGGTAAAGCCAAAGATGCAAATAATGGTAACCTACAATTAAGGAA; CAAAGAAGTGACTTGGGAAGTACTAGAAGGAGAGGTGGAAAAAGAAGCACTGAAGAAAAT; AATAGAAGACCAACAAGAATCCCTAAACAAATGGAAGTCAAAAGGTCGTAGATTTAAAGG; AAAAGGAAAGGGTAATAAAGCTGCCCAGCCTGGGTCTGGTAAAGGAAAAGTACAGTTTCA; GGGCAAGAAAACGAAATTTGCTAGTGATGATGAACATGATGAACATGATGAAAATGGTGC; AACTGGACCTGTGAAAAGAGCAAGAGAAGAAACAGACAAAGAAGAACCTGCATCCAAACA; ACAGAAAACAGAAAATGGTGCTGGAGACCAGTAGTTTAGTAAACCAATYTTTTATTCATT; TTAAATAGGTTTTAAACGACTTTTGTTTGCGGGGCTTTTAAAAGGAAAACCGAATTAGGT; CCACTTCAATGTCCACCTGTGAGAAAGGAAAAATTTTTTTGTTGTTTAACTTGTCTTTTT; GTTATGCAAATGAGATTTCTTTGAATGTATTGTTCTGTTTGTGTTATTTCAGATGATTCA; AATATCAAAAGGAAGATTCTTCCATTAAATTGCCTTTGTAATATGAGAATGTATTAGTAC; AAACTAACTAATAAAATATATACTATATGAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAA; AAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003142 unedited; TAATACGACTCACTATAGGGCGGCCGCGACATTCGCACGAGGCGTTGCTGTTGCTGTTTG; TGAGCCTGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGATAGCCGCAATGGCTGAAAA; TGGTGATAATGAAAAGATGGCTGCCCTGGAGGCCAAAATCTGTCATCAAATTGAGTATTA; TTTTGGCGACTTCAATTTGCCACGGGACAAGTTTCTAAAGGAACAGATAAAACTGGATGA; AGGCTGGGTACCTTTGGAGATAATGATAAAATTCAACAGGTTGAACCGTCTAACAACAGA; CTTTAATGTAATTGTGGAAGCATTGAGCAAATCCAAGGCAGAACTCATGGAAATCAGTGA; AGATAAAACTAAAATCAGAAGGTCTCCAAGCAAACCCCTACCTGAAGTGACTGATGAGTA; TAAAAATGATGTAAAAAACAGATCTGTTTATATTAAAGGCTTCCCAACTGATGCAACTCT; TGATGACATAAAAGAATGGTTAGAAGATAAAGGTCAAGTACTAAATATTCAGATGAGAAG; AACATTGCATAAAGCATTTAAGGGATCAATTTTTGTTGTGTTTGATAGCATTGAATCTGC; TAAGAAATTTGTAGAGACCCCTGGCCAGAAGTACAAAGAAACAGACCTGCTAATACTTTT; CAAGGACGATTACTTTGCCAAAAAAAATGAAGAAAGAAAACAAAATAAAGTGGAAGCTAA; ATTAAGAGCTAAACAGGAGCAAGAAGCAAAACAAAGTTAGAAGAGATGCTGAAATGAAAT; CTCTAGAAGAAAGATNGACTGCTGCTGAAATTTCGGTGATTTAGAGATCAGACTGTAGAG; AAGATTACACATACTTTNTNAAATATGNTGAATAAATGGATGACCTTGTCAGAGAGCAAG; AGGGGATAATTCATTTAAAGAAAAGCAAGNNAGCTTGGGTAAGCCAAGATGCAATATGGT; ACCTCANTAA;
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_003142 unedited; AACATGGTTAGAGAAAAGGTCAGTACTAAATACCTCAGATGAGAAGAACATTGCATAAAG; CATTTAAGGGATCAATTTTTGTTGTGTTTGATAGCATTGAATCTGCTAAGAAATTTGTAG; AGACCCCTGGCCAGAAGTACAAAGAAACAGACCTGCTAATACTTTTCAAGGACGATTACT; TTGCCAAAAAAAATGAAGAAAGAAAACAAAATAAAGTGGAAGCTAAATTAAGAGCTAAAC; AGGAGCAAGAAGCAAAACAAAAGTTAGAAGAAGATGCTGAAATGAAATCTCTAGAAGAAA; AGATTGGATGCTTGCTGAAATTTTCGGGTGATTTAGATGATCAGACCTGTAGAGAAGATT; TACACATACTTTTCTCAAATCATGGTGAAATAAAATGGATAGACTTCGTCAGAGGAGCAA; AAGAGGGGATAATTCTATTTAAAGAAAAAGCCAAGGAAGCATTGGGTAAAGCCAAAGATG; CAAATAATGGTAACCTACAATTAAGGAACAAAGAAGTGACTTGGGAAGTACTAGAAGGAG; AGGTGGAAAAAGAAGCACTGAAGAAAATAATAGAAGACCAACAAGAATCCCTAAACAAAT; GGAAGTCAAAAGGTCGTAGATTTAAAGGAAAAGGAAAGGGTAATAAAGCTGCCCAGCCTG; GGTCTGGTAAAGGAAAAGTACAGTTTCAGGGCAAGAAAACGAAATTTGCTAGTGATGATG; AACATGATGAACATGATGAAAATGGTGCAACTGGACCTGTGAAAAGAGCAAGAGAGAACA; GACAAAGAAGACCCTGCATCCAAACAACAGAAAACAGAAATGGTGCTGAGACAGTAGTTT; AGTAAACCAATTTTTATTCATTTTAAATAGTTTTAAACGACTTTTGTTGCGGGCTTTAAG; AAACGATAGTCACTCATGTCCACCTGTGAGAAAGAAATTTTTTTGTTGTTTAACTTGTTC; TTTTTGTTATGCA;
• Restriction Sites: NotI-NotI
• ACCN: NM_003142
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.NA
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003142.3, NP_003133.1
• RefSeq Size: 1673 bp
• RefSeq ORF: 1227 bp
• Locus ID: 6741
• UniProt ID: P05455
• Cytogenetics: 2q31.1
• Domains: LA, RRM
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Systemic lupus erythematosus
• Summary: The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein. [provided by RefSeq, Jun 2014]; Transcript Variant: This variant (1) and variant 2 encode the same protein.