SCAP (NM_012235) Human Untagged Clone

SKU SC315330

SCAP (untagged)-Human SREBF chaperone (SCAP)

Product Data

• Type: Human Untagged Clone
• Target Symbol: SCAP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012235 edited; AGGGTGATCCATGGTCACTTTCCGGGATGGCAGCAAGGTGACTTCGGCTGAGGATGACCC; TGACTGAAAGGCTGCGTGAGAAGATATCTCGGGCCTTCTACAACCATGGGCTCCTCTGTG; CATCCTATCCCATCCCCATCATCCTCTTCACAGGGTTCTGCATCTTAGCCTGCTGCTACC; CACTGCTGAAACTCCCCTTGCCAGGAACAGGACCTGTGGAATTCACCACCCCTGTGAAGG; ATTACTCGCCCCCACCTGTGGACTCTGACCGCAAACAAGGAGAGCCTACTGAGCAGCCTG; AGTGGTATGTGGGTGCCCCGGTGGCTTATGTCCAGCAGATATTTGTGAAGTCCTCAGTGT; TTCCCTGGCACAAGAACCTCCTGGCAGTAGATGTATTTCGTTCACCTTTGTCCCGGGCAT; TCCAACTGGTGGAGGAGATCCGGAACCACGTGCTGAGAGACAGCTCTGGGATCAGGAGCT; TGGAGGAGTTGTGTCTGCAAGTGACCGACCTGCTGCCAGGCCTTAGGAAGCTCAGGAACC; TACTCCCTGAGCATGGATGCCTGCTGCTGTCCCCTGGGAACTTCTGGCAGAATGACTGGG; AACGCTTCCATGCTGATCCTGACATCATTGGGACCATCCACCAGCACGAGCCTAAAACCC; TGCAGACTTCAGCCACACTCAAAGACTTGTTATTTGGTGTTCCTGGGAAGTACAGCGGGG; TGAGCCTCTACACCAGGAAGAGGATGGTCTCCTACACCATCACCCTGGTCTTCCAGCACT; ACCATGCCAAGTTCCTGGGCAGCCTGCGTGCCCGCCTGATGCTTCTGCACCCCAGCCCCA; ACTGCAGCCTTCGGGCGGAGAGCCTGGTCCACGTGCACTTCAAGGAGGAGATTGGTGTCG; CTGAGCTCATCCCCCTTGTGACCACCTACATCATCTTGTTTGCCTACATCTACTTCTCCA; CGCGGAAGATCGACATGGTCAAGTCCAAGTGGGGGCTGGCCCTGGCTGCCGTGGTCACAG; TGCTCAGCTCGCTGCTCATGTCTGTGGGACTCTGCACACTCTTCGGCCTGACGCCCACCC; TCAATGGCGGCGAGATTTTCCCCTACCTTGTGGTGGTTATTGGGTTAGAGAATGTGTTGG; TGCTCACCAAGTCTGTGGTCTCAACCCCGGTAGACCTGGAGGTGAAGCTGCGGATCGCCC; AAGGCCTAAGCAGCGAGAGCTGGTCCATCATGAAGAACATGGCCACGGAGCTGGGCATCA; TCCTCATCGGCTACTTCACCCTAGTGCCCGCCATCCAGGAGTTCTGTCTCTTTGCTGTCG; TGGGGCTGGTGTCTGACTTCTTCCTTCAGATGCTGTTTTTCACCACTGTCCTGTCCATTG; ACATTCGCCGGATGGAGCTAGCAGACCTGAACAAGCGACTGCCCCCTGAGGCCTGCCTGC; CCTCAGCCAAGCCAGTGGGGCAGCCAACGCGCTACGAGCGGCAGCTGGCTGTGAGGCCGT; CCACACCCCACACCATCACGTTGCAGCCGTCTTCCTTCCGAAACCTGCGGCTCCCCAAGA; GGCTGCGTGTTGTCTACTTCCTGGCCCGCACCCGCCTGGCACAGCGCCTCATCATGGCTG; GCACCGTTGTCTGGATTGGCATCCTGGTATACACAGACCCAGCAGGGCTGCGCAACTACC; TCGCTGCCCAGGTGACGGAACAGAGCCCATTGGGTGAGGGAGCCCTGGCTCCCATGCCCG; TGCCTAGTGGCATGCTGCCCCCCAGCCACCCGGACCCTGCCTTCTCCATCTTCCCACCTG; ATGCCCCTAAGCTACCTGAGAACCAGACGTCGCCAGGCGAGTCACCTGAGCGTGGAGGTC; CAGCAGAGGTTGTCCATGACAGCCCAGTCCCAGAGGTAACCTGGGGGCCTGAGGATGAGG; AACTTTGGAGGAAATTGTCCTTCCGCCACTGGCCGACGCTCTTCAGCTATTACAACATCA; CACTGGCCAAGAGGTACATCAGCCTGCTGCCCGTCATCCCAGTCACGCTCCGCCTGAACC; CGAGGGAGGCTCTGGAGGGCCGGCACCCTCAGGACGGCCGCAGTGCCTGGCCCCCACCGG; GGCCCATACCTGCTGGGCACTGGGAAGCAGGACCCAAGGGCCCAGGTGGGGTGCAGGCCC; ATGGAGACGTCACGCTGTACAAGGTGGCGGCGCTGGGCCTGGCCACCGGCATCGTCTTGG; TGCTGCTGCTGCTCTGCCTCTACCGCGTGCTATGCCCGCGCAACTACGGGCAGCTGGGTG; GTGGGCCCGGGCGGCGGAGGCGCGGGGAGCTGCCCTGCGACGACTACGGCTATGCGCCAC; CCGAGACGGAGATCGTGCCGCTTGTGCTGCGCGGCCACCTCATGGACATCGAGTGCCTGG; CCAGCGACGGCATGCTGCTGGTGAGCTGCTGCCTGGCAGGCCACATCTGCGTGTGGGACG; CGCAGACCGGGGATTGCCTAACGCGCATTCCGCGCCCAGGCAGGCAGCGCCGGGACAGTG; GCGTGGGCAGCGGGCTTGAGGCTCAGGAGAGCTGGGAACGACTTTCAGATGGTGGGAAGG; CTGGTCCAGAGGAGCCTGGGGACAGCCCTCCCCTGAGACACCGCCCCCGGGGCCCTCCGC; CGCCTTCCCTCTTCGGGGACCAGCCTGACCTCACCTGCTTAATTGACACCAACTTTTCAG; CGCAGCCTCGGTCCTCACAGCCCACTCAGCCCGAGCCCCGGCACCGGGCGGTCTGTGGCC; GCTCTCGGGACTCCCCAGGCTATGACTTCAGCTGCCTGGTGCAGCGGGTGTACCAGGAGG; AGGGGCTGGCGGCCGTCTGCACACCAGCCCTGCGCCCACCCTCGCCTGGGCCGGTGCTGT; CCCAGGCCCCTGAGGACGAGGGTGGCTCCCCCGAGAAAGGCTCCCCTTCCCTCGCCTGGG; CCCCCAGTGCCGAGGGTTCCATCTGGAGCTTGGAGCTGCAGGGCAACCTCATCGTGGTGG; GGCGGAGCAGCGGCCGGCTGGAGGTGTGGGACGCCATTGAAGGGGTGCTGTGCTGCAGCA; GCGAGGAGGTCTCCTCAGGCATTACCGCTCTGGTGTTCTTGGACAAAAGGATTGTGGCTG; CACGGCTCAACGGTTCCCTTGATTTCTTCTCCTTGGAGACCCACACTGCCCTCAGCCCCC; TGCAGTTTAGAGGGACCCCAGGGCGGGGCAGTTCCCCTGCCTCTCCAGTGTACAGCAGCA; GCGACACAGTGGCCTGTCACCTGACCCACACAGTGCCCTGTGCACACCAAAAACCCATCA; CAGCCCTGAAAGCCGCTGCTGGGCGCTTGGTGACTGGGAGCCAAGACCACACACTGAGAG; TGTTCCGTCTGGAGGACTCGTGCTGCCTCTTCACCCTTCAGGGCCACTCAGGGGCCATCA; CGACCGTGTACATTGACCAGACCATGGTGCTGGCCAGTGGAGGACAAGATGGGGCCATCT; GCCTGTGGGATGTACTGACTGGCAGCCGGGTCAGCCATGTGTTTGCTCACCGTGGGGATG; TCACCTCCCTTACCTGTACCACCTCCTGTGTCATCAGCAGTGGCCTGGATGACCTCATCA; GCATCTGGGACCGCAGCACAGGCATCAAGTTCTACTCCATTCAGCAGGACCTGGGCTGTG; GTGCAAGCTTGGGTGTCATCTCAGACAACCTGCTGGTGACTGGCGGCCAGGGCTGTGTCT; CCTTTTGGGACCTAAACTACGGGGACCTGTTACAGACAGTCTACCTGGGGAAGAACAGTG; AGGCCCAGCCTGCCCGCCAGATCCTGGTGCTGGACAACGCTGCCATTGTCTGCAACTTTG; GCAGTGAGCTCAGCCTGGTGTATGTGCCCTCTGTGCTGGAGAAGCTGGACTGAGCGCAGG; GCCTCCTTGCCCAGGCAGGAGGCTGGGGTGCTGTGTGGGGGCCAATGCACTGAACCTGGA; CTTGGGGGAAAGAGCCGAGTATCTTCCAGCCGCTGCCTCCTGACTGTAATAATATTAAAC; TTTTTTAAAAAACCATAAAAAAAAAAAAAAAAAAAAAAAAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_012235
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: It is not a varient.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012235.2, NP_036367.2
• RefSeq Size: 4255 bp
• RefSeq ORF: 3840 bp
• Locus ID: 22937
• UniProt ID: Q12770
• Cytogenetics: 3p21.31
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Summary: This gene encodes a protein with a sterol sensing domain (SSD) and seven WD domains. In the presence of cholesterol, this protein binds to sterol regulatory element binding proteins (SREBPs) and mediates their transport from the ER to the Golgi. The SREBPs are then proteolytically cleaved and regulate sterol biosynthesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).