CD11b (ITGAM) (NM_000632) Human Untagged Clone
SKU
SC315229
ITGAM (untagged)-Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2
$1,004.00
In Stock*
Specifications
| Product Data | |
| Type | Human Untagged Clone |
|---|---|
| Target Symbol | CD11b |
| Synonyms | CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | |
| Restriction Sites | Please inquire | |
| ACCN | NM_000632 |
| Insert Size | 3700 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000632.2, NP_000623.1 |
| RefSeq Size | 4740 bp |
| RefSeq ORF | 3459 bp |
| Locus ID | 3684 |
| UniProt ID | P11215 |
| Cytogenetics | 16p11.2 |
| Domains | FG-GAP, VWA |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Leukocyte transendothelial migration, Regulation of actin cytoskeleton |
| Summary | This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Mar 2009 Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The resulting isoform (2) lacks one internal amino acid, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000632" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC221743 | ITGAM (Myc-DDK-tagged)-Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2 | 10 ug |
$1,003.00
|
|
| RC221743L1 | Lenti ORF clone of Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,303.00
|
|
| RC221743L2 | Lenti ORF clone of Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2, mGFP tagged | 10 ug |
$1,303.00
|
|
| RC221743L3 | Lenti ORF clone of Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2, Myc-DDK-tagged | 10 ug |
$1,303.00
|
|
| RC221743L4 | Lenti ORF clone of Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2, mGFP tagged | 10 ug |
$1,303.00
|
|
| RG221743 | ITGAM (tGFP-tagged) - Human integrin, alpha M (complement component 3 receptor 3 subunit) (ITGAM), transcript variant 2 | 10 ug |
$789.00
$1,203.00
|
|
Citations
Combined protein- and nucleic acid-level effects of rs1143679 (R77H), a lupus-predisposing variant within ITGAM
Maiti, Kim-Howard, Motghare et al
Hum. Mol. Genet. (2014)
Maiti, Kim-Howard, Motghare et al
Hum. Mol. Genet. (2014)
Cited by 35
AI Summary: OriGene Technologies provided the full-length ITGAM cDNA (containing the rs1143679 ‘G’ allele) used in this study to investigate the genetic and molecular effects of the lupus-associated variant.
Snippet: Full-length cDNA of ITGAM (containing the rs1143679 ‘G’ allele) was obtained from Origene Technologies, Rockville, MD, USA (10) in pBR322. ITGAM cDNA was PCR amplified (forward primer-attctgacgcgtatgctctcagagtccttctg and reverse primer-ttgtaaatatcgatactggggttcggccc, amplicon size 3470 bp) and cloned into the MluI-ClaI multiple cloning site (MCS1) of the pVITRO2-neo-MCS dual expression vector (Invivogen, USA) for expression in mammalian cells. …
Phagocytosis is the main CR3-mediated function affected by the lupus-associated variant of CD11b in human myeloid cells
Fossati-Jimack, Ling, Cortini et al
PLoS ONE (2013) 8 (2), e57082 DOI: 10.1371/journal.pone.0057082
Fossati-Jimack, Ling, Cortini et al
PLoS ONE (2013) 8 (2), e57082 DOI: 10.1371/journal.pone.0057082
Cited by 79
AI Summary: OriGene's CD11b cDNA clone (SC315229, NM-000632.2) was used in this study to generate macrophage cell lines expressing the 77R allele, enabling direct comparison of CR3-mediated functions between genetic variants in human myeloid cells.
Snippet: in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS), 2 mM L-glutamine, 1% penicillin/streptomycin (Life Technologies, Grand ... The cDNA of the 77R CD11b allele was obtained from OriGene Technologies (Rockville, MD, USA, SC315229, NM-000632.2), whilst
Showing 1-2 of 2 papers.
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