ASPP1 (PPP1R13B) (NM_015316) Human Untagged Clone

SKU SC315169

PPP1R13B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B)

Product Data

• Type: Human Untagged Clone
• Target Symbol: ASPP1
• Synonyms: ASPP1; p53BP2-like; p85
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315169 representing NM_015316.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGATGCCGATGATATTAACTGTTTTCTTGAGCAACAATGAACAGATTTTAACAGAAGTTCCTATAACA; CCGGAAACAACCTGTCGAGATGTTGTAGAATTTTGCAAGGAACCTGGAGAAGGCAGCTGCCATTTAGCT; GAAGTGTGGAGGGGAAATGAACGTCCCATACCCTTTGATCATATGATGTACGAACATCTTCAGAAATGG; GGTCCACGGAGGGAAGAAGTGAAATTTTTCCTTCGACACGAGGACTCCCCAACTGAGAACAGTGAACAA; GGTGGCCGTCAGACCCAAGAGCAACGAACTCAGAGAAATGTAATAAATGTACCTGGAGAAAAACGTACT; GAAAATGGGGTTGGGAATCCACGTGTTGAACTTACCCTCTCAGAGCTCCAAGATATGGCAGCTAGGCAA; CAGCAGCAGATTGAAAATCAGCAGCAGATGTTGGTTGCCAAGGAACAGCGTTTACATTTTCTAAAGCAA; CAGGAGCGCCGTCAGCAGCAGTCTATTTCTGAAAATGAAAAGCTTCAGAAATTGAAAGAACGAGTTGAA; GCCCAGGAGAACAAGCTGAAGAAAATTCGTGCAATGAGAGGACAAGTCGACTACAGCAAAATCATGAAC; GGCAATCTGTCTGCTGAAATAGAAAGGTTCAGTGCCATGTTCCAGGAAAAGAAGCAGGAAGTACAGACT; GCAATTTTAAGGGTTGATCAGCTTAGTCAGCAATTGGAAGATTTAAAGAAAGGAAAACTGAATGGGTTC; CAGTCTTACAATGGCAAATTGACGGGACCAGCGGCGGTGGAGTTAAAAAGACTGTACCAAGAACTACAG; ATTCGTAACCAACTTAACCAGGAACAAAATTCAAAACTTCAGCAGCAGAAGGAACTCTTAAATAAGCGC; AACATGGAGGTGGCCATGATGGACAAGCGAATCAGTGAACTGCGTGAACGTCTCTATGGGAAAAAAATT; CAGCTGAACCGTGTGAATGGCACGTCATCACCACAGTCCCCTCTGAGCACATCGGGCAGGGTCGCTGCT; GTGGGGCCTTATATCCAGGTTCCCAGTGCCGGAAGCTTTCCTGTGCTGGGGGACCCTATAAAGCCCCAG; TCTCTCAGTATTGCCTCAAATGCTGCTCATGGAAGATCCAAATCCGCTAATGATGGAAACTGGCCAACA; TTAAAACAGAATTCTAGCTCTTCCGTGAAACCAGTGCAGGTGGCCGGTGCAGACTGGAAGGATCCGAGC; GTGGAGGGGTCTGTCAAGCAGGGCACTGTCTCCAGCCAGCCTGTGCCCTTCTCAGCACTGGGACCCACG; GAGAAGCCGGGCATCGAGATTGGTAAAGTGCCACCTCCCATCCCGGGTGTAGGCAAGCAGCTGCCTCCA; AGCTATGGGACATACCCAAGTCCTACACCTCTGGGTCCTGGGTCGACAAGCTCCCTGGAAAGGAGGAAG; GAAGGCAGCTTGCCCAGGCCCAGTGCAGGCCTGCCAAGTCGACAGAGGCCCACCCTGCTGCCCGCCACA; GGCAGCACCCCCCAGCCAGGCTCCTCACAACAGATTCAGCAGAGGATTTCCGTACCGCCAAGTCCCACG; TACCCGCCAGCGGGACCACCTGCATTTCCAGCTGGGGACAGCAAGCCTGAACTCCCACTGACAGTGGCC; ATTAGGCCTTTCCTGGCTGATAAAGGGTCAAGGCCACAGTCTCCCAGGAAAGGACCCCAGACAGTGAAT; TCAAGTTCCATATACTCCATGTACCTCCAGCAAGCCACACCACCTAAGAATTACCAGCCGGCAGCACAC; AGCGCCTTAAATAAGTCAGTTAAAGCAGTGTATGGTAAGCCCGTTTTACCTTCGGGTTCAACCTCTCCA; TCGCCGCTGCCGTTTCTTCACGGGTCACTGTCCACGGGCACACCACAGCCTCAGCCACCTTCAGAAAGT; ACTGAGAAAGAGCCTGAGCAGGATGGCCCCGCCGCCCCCGCAGATGGCAGCACCGTGGAGAGCCTGCCA; CGGCCACTCAGCCCCACCAAGCTCACGCCCATCGTGCATTCGCCACTGCGCTACCAGAGTGATGCAGAC; CTGGAGGCCCTCCGCAGGAAGCTGGCCAACGCGCCCCGGCCCCTGAAAAAGCGCAGCTCCATCACAGAG; CCCGAGGGCCCCGGCGGGCCCAACATCCAGAAGCTGCTGTACCAGCGCTTCAACACCCTGGCCGGTGGC; ATGGAGGGCACCCCTTTCTACCAGCCCAGCCCCTCCCAGGACTTCATGGGCACCTTGGCCGATGTGGAC; AATGGAAACACCAATGCCAATGGAAACCTGGAAGAGCTCCCCCCTGCCCAGCCCACAGCCCCACTCCCC; GCTGAGCCTGCCCCGTCATCAGATGCCAATGATAATGAGTTACCTTCCCCCGAACCAGAGGAGCTCATC; TGTCCCCAAACCACCCACCAAACTGCCGAGCCGGCAGAGGACAATAACAACAACGTGGCCACGGTCCCC; ACCACGGAGCAGATCCCGAGTCCTGTGGCTGAGGCCCCATCTCCAGGGGAAGAGCAGGTCCCTCCAGCA; CCTCTTCCCCCTGCCAGCCACCCTCCTGCCACCTCCACGAACAAGCGGACCAACTTGAAGAAGCCCAAC; TCGGAGCGGACGGGGCACGGGCTGAGAGTCCGGTTTAACCCCCTGGCACTGCTCCTAGACGCGTCTCTG; GAAGGAGAGTTCGATCTGGTGCAGAGGATCATCTATGAGGTGGAAGATCCCAGCAAGCCCAACGACGAA; GGGATCACCCCACTGCACAACGCCGTCTGCGCCGGCCACCATCACATCGTGAAGTTCCTGCTGGATTTT; GGTGTCAACGTGAATGCTGCTGATAGTGATGGATGGACGCCGCTGCACTGCGCTGCCTCTTGTAACAGC; GTTCACCTCTGCAAACAGCTGGTGGAGAGTGGTGCCGCCATTTTTGCCTCAACCATAAGCGACATTGAA; ACTGCTGCAGACAAGTGTGAGGAGATGGAGGAAGGCTACATCCAGTGCTCCCAGTTTCTATATGGGGTG; CAGGAAAAGCTGGGTGTGATGAACAAAGGTGTGGCGTATGCTCTGTGGGACTACGAGGCCCAGAACAGT; GACGAGCTGTCCTTCCACGAAGGGGACGCCCTCACCATCCTGAGGCGCAAGGACGAAAGCGAGACTGAG; TGGTGGTGGGCTCGCCTTGGAGACCGGGAGGGCTATGTGCCCAAAAACCTGCTGGGGCTGTATCCACGG; ATCAAACCCCGACAGCGAACACTCGCCTGA; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT; ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA;
• Restriction Sites: SgfI-RsrII
• ACCN: NM_015316
• Insert Size: 3273 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015316.1
• RefSeq Size: 4976 bp
• RefSeq ORF: 3273 bp
• Locus ID: 23368
• UniProt ID: Q96KQ4
• Cytogenetics: 14q32.33
• Domains: ANK, SH3
• Protein Families: Druggable Genome
• MW: 119.6 kDa
• Summary: This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor. [provided by RefSeq, Jul 2008]