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Synapsin I (SYN1) (NM_133499) Human Untagged Clone

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SKU
SC314376
SYN1 (untagged)-Human synapsin I (SYN1), transcript variant Ib
  $675.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol Synapsin I
Synonyms EPILX; MRX50; SYN1a; SYN1b; SYNI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC314376 representing NM_133499.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAACTACCTGCGGCGCCGCCTGTCGGACAGCAACTTTATGGCCAATCTGCCAAATGGGTACATGACA
GACCTGCAGCGTCCGCAGCCGCCCCCACCGCCGCCCGGTGCCCACAGCCCCGGAGCCACGCCCGGTCCC
GGGACCGCCACTGCCGAGAGGTCCTCCGGGGTCGCCCCAGCGGCCTCTCCGGCCGCCCCTAGCCCCGGG
TCCTCGGGGGGCGGTGGCTTCTTCTCGTCGCTGTCCAACGCGGTCAAGCAGACCACGGCGGCGGCAGCT
GCCACCTTCAGCGAGCAGGTGGGCGGCGGCTCTGGGGGCGCAGGCCGCGGGGGAGCCGCCTCCAGGGTG
CTGCTGGTCATCGACGAGCCGCACACCGACTGGGCAAAATACTTCAAAGGGAAAAAGATCCATGGAGAA
ATTGACATTAAAGTAGAACAGGCCGAATTCTCTGATCTCAACCTTGTGGCCCATGCCAATGGTGGATTC
TCTGTGGATATGGAAGTTCTTCGGAATGGGGTGAAGGTCGTGCGGTCTCTGAAGCCGGATTTTGTGCTG
ATCCGCCAGCACGCCTTCAGCATGGCACGCAACGGAGACTACCGCAGTTTGGTCATTGGGCTGCAGTAT
GCTGGAATCCCCAGTGTTAACTCCTTGCATTCTGTCTACAACTTCTGTGACAAGCCCTGGGTGTTTGCC
CAGATGGTTCGACTGCATAAGAAACTGGGGACAGAAGAATTCCCTCTAATTGATCAGACCTTCTACCCC
AATCACAAAGAAATGCTCAGCAGTACAACGTACCCCGTGGTTGTGAAGATGGGGCACGCACACTCTGGG
ATGGGCAAGGTCAAGGTTGACAACCAGCATGACTTCCAGGACATCGCAAGTGTCGTGGCACTGACCAAG
ACGTATGCCACTGCCGAGCCCTTCATCGATGCCAAATATGACGTGCGTGTCCAGAAGATTGGGCAGAAC
TACAAGGCCTACATGAGGACGTCAGTGTCAGGGAACTGGAAGACCAATACTGGCTCTGCGATGCTGGAG
CAAATTGCCATGTCTGACAGATACAAGCTGTGGGTGGACACGTGCTCAGAGATTTTTGGGGGACTGGAC
ATCTGCGCAGTGGAAGCGCTACATGGCAAGGACGGAAGGGATCACATCATTGAGGTGGTGGGTTCCTCC
ATGCCGCTCATTGGTGACCACCAGGATGAAGACAAACAGCTCATCGTAGAGCTCGTGGTCAACAAGATG
GCTCAGGCCCTGCCCCGGCAGCGACAGCGGGATGCCTCCCCTGGCAGGGGCTCCCATGGCCAGACTCCG
TCCCCAGGGGCCCTGCCCTTGGGCCGCCAGACCTCCCAGCAGCCCGCAGGGCCCCCGGCTCAGCAGCGA
CCCCCACCACAGGGCGGCCCTCCACAGCCGGGTCCAGGCCCCCAGCGCCAGGGACCCCCATTGCAGCAG
CGCCCGCCCCCGCAGGGCCAGCAGCACCTTTCAGGCCTTGGACCCCCAGCTGGCAGCCCCCTGCCCCAG
CGCCTTCCAAGTCCCACCTCAGCGCCCCAGCAGCCCGCGTCCCAGGCCGCGCCGCCGACCCAGGGTCAA
GGCCGCCAATCCCGGCCAGTGGCGGGAGGCCCCGGGGCGCCTCCAGCAGCCCGCCCGCCCGCCTCTCCG
TCTCCCCAGCGCCAGGCGGGCCCCCCACAGGCTACCCGTCAGACATCCGTCTCTGGCCCGGCTCCGCCA
AAGGCCTCTGGGGCCCCACCGGGCGGGCAGCAGCGCCAGGGCCCGCCCCAGAAACCCCCAGGCCCAGCC
GGCCCCACACGCCAGGCCAGCCAGGCGGGTCCCGTGCCCCGCACTGGGCCACCCACCACGCAGCAGCCT
CGGCCCAGCGGCCCGGGCCCCGCTGGACGTCCCAAACCACAGCTGGCCCAGAAACCCAGCCAGGACGTG
CCGCCACCCGCCACCGCCGCTGCAGGGGGACCTCCGCACCCCCAGCTCAAAGCCAGCCCCGCCCAGGCC
CAGCCTTAG
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_133499
Insert Size 2010 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133499.2
RefSeq Size 3172 bp
RefSeq ORF 2010 bp
Locus ID 6853
UniProt ID P17600
Cytogenetics Xp11.3-p11.23
MW 70 kDa
Summary This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
Transcript Variant: This variant (Ib) utilizes an alternative splice acceptor site in terminal exon compared to variant Ia, resulting in the lack of an internal segment and a frameshift. This variant encodes isoform Ib, which contains a shorter carboxyl terminus and a distinct domain F, as compared to isoform Ia.
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Documents
Resources
"NM_133499" in other vectors (4)
SKU Description Size Price
RC217800 SYN1 (Myc-DDK-tagged)-Human synapsin I (SYN1), transcript variant Ib 10 ug
$613.00
RC217800L3 Lenti-ORF clone of SYN1 (Myc-DDK-tagged)-Human synapsin I (SYN1), transcript variant Ib 10 ug
$913.00
RC217800L4 Lenti-ORF clone of SYN1 (mGFP-tagged)-Human synapsin I (SYN1), transcript variant Ib 10 ug
$913.00
RG217800 SYN1 (tGFP-tagged) - Human synapsin I (SYN1), transcript variant Ib 10 ug
$813.00

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