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RHBDL1 (NM_003961) Human Untagged Clone

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SKU
SC313471
RHBDL1 (untagged)-Human rhomboid, veinlet-like 1 (Drosophila) (RHBDL1)
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol RHBDL1
Synonyms RHBDL; RRP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>SC313471 representing NM_003961.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGTAGGGTGGAAGACGGGGGAACAACTGAGGAGCTGGAGGACTGGGACCCAGGCACCAGTGCCCTG
CCAGCTCCTGGGATCAAGCAGGGTCCCAGGGAACAGACAGGCACGGGGCCCCTGTCCCAAAAGTGCTGG
GAGCCTGAGCCTGATGCTCCCAGCCAGCCTGGCCCAGCCCTTTGGTCCAGGGGTCGGGCCCGCACTCAG
GCCTTGGCTGGCGGCTCCTCACTGCAGCAGCTGGACCCCGAGAACACAGGCTTCATCGGTGCGGACACC
TTCACTGGCCTGGTGCACAGCCATGAGCTGCCCCTGGACCCGGCCAAGCTGGACATGCTGGTGGCCCTG
GCTCAGAGCAACGAGCAGGGCCAGGTCTGCTACCAGGAGCTGGTGGACCTGATCAGCAGCAAGCGCTCC
AGCAGTTTCAAGCGGGCCATTGCTAACGGACAGCGGGCACTGCCCCGGGACGGGCCGCTGGATGAGCCA
GGCCTAGGTGTCTACAAGCGGTTTGTGCGTTACGTGGCCTACGAGATCCTGCCTTGTGAGGTGGACCGC
CGCTGGTACTTCTACCGTCACCGCAGCTGCCCACCCCCCGTGTTCATGGCCTCGGTCACTCTTGCCCAG
ATCATCGTGTTCCTGTGTTACGGGGCCCGCCTCAACAAGTGGGTGCTGCAGACCTACCACCCCGAGTAC
ATGAAGAGCCCCCTTGTGTACCACCCCGGGCACCGTGCCCGCGCCTGGCGCTTCCTCACCTACATGTTC
ATGCACGTTGGGCTGGAGCAGCTGGGGTTCAACGCCCTCCTGCAGCTGATGATCGGGGTGCCCCTGGAG
ATGGTGCACGGCCTGCTCCGCATCAGCCTGCTCTACCTGGCAGGCGTGCTGGCAGGCTCCCTAACCGTC
TCCATCACCGACATGCGGGCCCCGGTGGTGGGAGGCTCCGGCGGGGTCTACGCCCTGTGCTCGGCACAC
CTGGCCAACGTTGTCATGAACTGGGCTGGGATGAGATGTCCCTACAAGTTGCTGAGGATGGTGCTGGCC
TTGGTGTGCATGAGCTCCGAGGTGGGCCGGGCCGTGTGGCTGCGCTTCTCCCCGCCGCTGCCCGCCTCG
GGCCCACAGCCCAGCTTCATGGCGCACCTGGCAGGCGCGGTGGTGGGGGTGAGCATGGGCCTGACCATC
CTGCGGAGCTACGAGGAGCGCCTGCGGGACCAGTGCGGCTGGTGGGTGGTGCTGCTGGCCTACGGCACC
TTCCTGCTCTTCGCCGTCTTCTGGAACGTCTTCGCCTACGACCTGCTGGGCGCCCACATCCCCCCACCG
CCCTGA
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites SgfI-MluI |
ACCN NM_003961
Insert Size 1317 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003961.1
RefSeq Size 1559 bp
RefSeq ORF 1317 bp
Locus ID 9028
Cytogenetics 16p13.3
Protein Families Protease, Transmembrane
MW 48.3 kDa
Summary This gene encodes a protein similar to Rhomboid in Drosophila which is involved in signalling in the Spitz/epidermal growth factor receptor/mitogen-activated protein kinase pathway. The Rhomboid family of proteins consists of intramembrane serine proteases containing several transmembrane domains. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2013
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"NM_003961" in other vectors (6)
SKU Description Size Price
RC221768 RHBDL1 (Myc-DDK-tagged)-Human rhomboid, veinlet-like 1 (Drosophila) (RHBDL1) 10 ug
$457.00
RC221768L1 Lenti ORF clone of Human rhomboid, veinlet-like 1 (Drosophila) (RHBDL1), Myc-DDK-tagged 10 ug
$757.00
RC221768L2 Lenti ORF clone of Human rhomboid, veinlet-like 1 (Drosophila) (RHBDL1), mGFP tagged 10 ug
$757.00
RC221768L3 Lenti ORF clone of Human rhomboid, veinlet-like 1 (Drosophila) (RHBDL1), Myc-DDK-tagged 10 ug
$757.00
RC221768L4 Lenti ORF clone of Human rhomboid, veinlet-like 1 (Drosophila) (RHBDL1), mGFP tagged 10 ug
$757.00
RG221768 RHBDL1 (tGFP-tagged) - Human rhomboid, veinlet-like 1 (Drosophila) (RHBDL1) 10 ug
$489.00 $657.00

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