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BRIT1 (MCPH1) (NM_024596) Human Untagged Clone

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SKU
SC311118
MCPH1 (untagged)-Human microcephalin 1 (MCPH1), transcript variant 1
  $679.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol BRIT1
Synonyms BRIT1; MCT
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene ORF sequence for NM_024596 edited
ATGGCGGCCCCCATCCTGAAAG::::::TGGCCTATGTTGAAGTGTGGTCATCCAATGGA
ACAGAAAATTATTCAAAGACATTTACAACACAGCTTGTGGATATGGGGGCAAAGGTTTCA
AAAACTTTTAACAAACAAGTAACTCACGTTATCTTCAAAGATGGCTACCAGAGCACTTGG
GACAAAGCTCAGAAGAGAGGCGTAAAGCTCGTTTCGGTGCTCTGGGTTGAAAAATGCAGG
ACAGCTGGAGCACACATTGATGAATCATTGTTCCCTGCAGCTAATATGAATGAACACTTA
TCAAGCCTAATTAAAAAAAAACGTAAATGTATGCAGCCCAAAGATTTTAATTTTAAAACA
CCAGAAAATGATAAGAGATTTCAGAAGAAATTTGAGAAAATGGCTAAAGAGCTACAAAGG
CAAAAAACAAATCTAGATGATGATGTACCTATTCTCTTATTTGAATCTAATGGTTCATTA
ATATATACTCCCACAATTGAAATTAATAGTAGTCACCACAGCGCAATGGAGAAGAGATTA
CAAGAGATGAAGGAGAAAAGGGAAAATCTTTCCCCCACCTCTTCCCAAATGATTCAGCAG
TCTCATGATAATCCAAGTAACTCTCTGTGTGAAGCACCTTTGAACATTTCACGTGATACT
TTGTGTTCAGATGAATACTTTGCTGGTGGCTTACACTCATCTTTTGATGATCTTTGTGGA
AACTCAGGATGTGGAAATCAGGAAAGGAAGTTGGAAGGATCCATTAATGACATTAAAAGT
GATGTGTGTATTTCTTCACTTGTATTGAAAGCAAATAATATTCATTCATCACCATCTTTC
ACTCACCTCGATAAATCAAGTCCTCAGAAATTTCTGAGTAATCTTTCAAAGGAAGAAATA
AACTTGCAAAGAAATATTGCAGGTAAAGTAGTCACCCCTSACCAAAAGCAGGCTGCAGGT
ATGTCTCAGGAGACGTTTGAAGAGAAGTATCGTTTGTCTCCTACCTTATCTTCAACAAAA
GGCCACCTTTTGATACATTCAAGACCCAGGAGTTCCTCAGTAAAGAGAAAAAGAGTATCA
CATGGCTCCCATTCACCTCCGAAGGAAAAATGCAAGAGAAAGAGGAGCACCAGGAGATCT
ATCATGCCGAGGCTGCAGCTGTGCAGGTCGGAAGRCAGGCTGCAGCACGTGGCGGGACCT
GCCCTGGAGGCTCTTAGCTGTGGGGAGTCTTCATATGATGACTATTTTTCACCTGATAAT
CTTAAGGAAAGGTATTCAGAGAATCTTCCTCCTGAATCTCAGCTGCCATCAAGCCCTGCT
CAGTTGAGCTGCAGAAGTCTTTCTAAGAAGGAGAGAACAAGCATATTTGAAATGTCTGAT
TTTTCCTGCGTTGGCAAAAAAACCAGAACAGTTGACATTACCAATTTCACAGCAAAAACC
ATCTCCAGTCCTCGGAAAACTGGAAATGGTGAAGGCCGTGCAACTTCGAGTTGCGTGACT
TCTGCCCCTGAAGAAGCCCTAAGGTGTTGTAGACAGGCTGGGAAAGAAGACGCATGCCCA
GAGGGAAATGGCTTTTCTTACACCATTGAGGACCCTGCTCTTCCAAAAGGACATGATGAT
GATTTAACTCCTTTGGAAGGAAGCCTTGAAGAAATGAAAGAAGCGGTTGGTCTGAAAAGC
ACACAGAACAAAGGTACCACTTCCAAAATATCAAACTCCTCTGAAGGCGAAGCCCAGAGT
GAACATGAGCCATGTTTTATAGTTGACTGTAACATGGAGACGTCTACAGAAGAGAAGGAA
AACTTACCCGGAGGATACAGTGGAAGTGTTAAAAATAGACCAACAAGGCATGATGTTTTA
GATGACTCATGTGACGGCTTTAAGGACCTCATCAAACCTCATGAGGAATTGAAGAAAAGT
GGGAGAGGCAAAAAGCCAACAAGAACATTAGTCATGACAAGCATGCCATCTGAAAAGCAG
AATGTCGTCATCCAGGTTGTGGATAAATTGAAAGGCTTTTCAATTGCACCAGACGTCTGT
GAGACCACGACTCACGTGCTTTCCGGGAAGCCACTTCGCACCCTGAATGTGCTGCTGGGA
ATTGCGCGTGGCTGCTGGGTTCTCTCTTATGATTGGGTGCTATGGTCTTTAGAATTGGGT
CACTGGATTTCTGAGGAGCCGTTCGAACTGTCTCACCACTTCCCTGCAGCTCCCCTGTGC
CGAAGCGAGTGCCACTTGTCTGCAGGGCCGTACCGCGGAACCCTCTTTGCCGACCAGCCA
GCGATGTTTGTCTCGCCTGCCAGCAGCCCCCCAGTGGCCAAGCTCTGTGAACTAGTCCAC
CTGTGCGGAGGCCGGGTCAGCCAAGTCCCCCGCCAGGCCAGCATCGTCATCGGGCCCTAC
AGCGGAAAGAAGAAAGCCACAGTCAAGTATCTGTCTGAGAAATGGGTCTTAGATTCCATC
ACCCAGCACAAGGTCTGTGCCCCTGAAAACTACCTATTGTCACAATGA
Restriction Sites Please inquire |
ACCN NM_024596
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to be a good match to NM_024596.1 except for 6bp deletion at 5' and one SNP.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024596.1, NP_078872.1
RefSeq Size 2518 bp
RefSeq ORF 2223 bp
Locus ID 79648
UniProt ID Q8NEM0
Cytogenetics 8p23.1
Domains BRCT
Summary This gene encodes a DNA damage response protein. The encoded protein may play a role in G2/M checkpoint arrest via maintenance of inhibitory phosphorylation of cyclin-dependent kinase 1. Mutations in this gene have been associated with primary autosomal recessive microcephaly 1 and premature chromosome condensation syndrome. Alternatively spliced transcript variants have been described. provided by RefSeq, Feb 2010
Transcript Variant: This variant (1, also known as MCPH1-FL) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
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Documents
Resources
"NM_024596" in other vectors (8)
SKU Description Size Price
RC224920 MCPH1 (Myc-DDK-tagged)-Human microcephalin 1 (MCPH1), transcript variant 1 10 ug
$765.00
RC224920L1 Lenti ORF clone of Human microcephalin 1 (MCPH1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,065.00
RC224920L2 Lenti ORF clone of Human microcephalin 1 (MCPH1), transcript variant 1, mGFP tagged 10 ug
$1,065.00
RC224920L3 Lenti ORF clone of Human microcephalin 1 (MCPH1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,065.00
RC224920L4 Lenti ORF clone of Human microcephalin 1 (MCPH1), transcript variant 1, mGFP tagged 10 ug
$1,065.00
RG224920 MCPH1 (tGFP-tagged) - Human microcephalin 1 (MCPH1), transcript variant 1 10 ug
$789.00 $965.00
SC318315 MCPH1 (untagged)-Human microcephalin 1 (MCPH1), transcript variant 1 10 ug
$842.00
SC318316 MCPH1 (untagged)-Human microcephalin 1 (MCPH1), transcript variant 1 10 ug
$842.00

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