VRK3 (NM_016440) Human Untagged Clone

SKU SC310347

VRK3 (untagged)-Human vaccinia related kinase 3 (VRK3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: VRK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310347 representing NM_016440.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGATCTCCTTCTGTCCAGACTGTGGCAAAAGTATCCAAGCGGCATTCAAATTCTGCCCCTACTGTGGA; AATTCTTTGCCTGTAGAGGAGCATGTAGGGTCCCAGACCTTTGTCAATCCACATGTGTCATCCTTCCAA; GGCTCAAAGAGAGGGCTGAACTCCAGTTTTGAAACCTCTCCTAAGAAAGTGAAATGGTCCAGCACCGTC; ACCTCTCCCCGATTATCCCTCTTCTCAGATGGTGACAGTTCTGAGTCTGAAGATACTCTGAGTTCCTCT; GAGAGATCCAAAGGCTCCGGGAGCAGACCCCCAACCCCCAAAAGCAGCCCTCAGAAGACCAGGAAGAGC; CCTCAGGTGACCAGGGGTAGCCCTCAGAAGACCAGCTGTAGCCCTCAGAAGACCAGGCAGAGCCCTCAG; ACGCTGAAGCGGAGCCGAGTGACCACCTCACTTGAAGCTTTGCCCACAGGGACAGTGCTGACAGACAAG; AGTGGGCGACAGTGGAAGCTGAAGTCCTTCCAGACCAGGGACAACCAGGGCATTCTCTATGAAGCTGCA; CCCACCTCCACCCTCACCTGTGACTCAGGACCACAGAAGCAAAAGTTCTCACTCAAACTGGATGCCAAG; GATGGGCGCTTGTTCAATGAGCAGAACTTCTTCCAGCGGGCCGCCAAGCCTCTGCAAGTCAACAAGTGG; AAGAAGCTGTACTCGACCCCACTGCTGGCCATCCCTACCTGCATGGGTTTCGGTGTTCACCAGGACAAA; TACAGGTTCTTGGTGTTACCCAGCCTGGGGAGGAGCCTTCAGTCGGCCCTGGATGTCAGCCCAAAGCAT; GTGCTGTCAGAGAGGTCTGTGCTGCAGGTGGCCTGCCGGCTGCTGGATGCCCTGGAGTTCCTCCATGAG; AATGAGTATGTTCATGGAAATGTGACAGCTGAAAATATCTTTGTGGATCCAGAGGACCAGAGTCAGGTG; ACTTTGGCAGGCTATGGCTTCGCCTTCCGCTATTGCCCAAGTGGCAAACACGTGGCCTACGTGGAAGGC; AGCAGGAGCCCTCACGAGGGGGACCTTGAGTTCATTAGCATGGACCTGCACAAGGGATGCGGGCCCTCC; CGCCGCAGCGACCTCCAGAGCCTGGGCTACTGCATGCTGAAGTGGCTCTACGGGTTTCTGCCATGGACA; AATTGCCTTCCCAACACTGAGGACATCATGAAGCAAAAACAGAAGTTTGTTGATAAGCCGGGGCCCTTC; GTGGGACCCTGCGGTCACTGGATCAGGCCCTCAGAGACCCTGCAGAAGTACCTGAAGGTGGTGATGGCC; CTCACGTATGAGGAGAAGCCGCCCTACGCCATGCTGAGGAACAACCTAGAAGCTTTGCTGCAGGATCTG; CGTGTGTCTCCATATGACCCCATTGGCCTCCCGATGGTGCCCTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_016440
• Insert Size: 1425 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_016440.3
• RefSeq Size: 2129 bp
• RefSeq ORF: 1425 bp
• Locus ID: 51231
• UniProt ID: Q8IV63
• Cytogenetics: 19q13.33
• Domains: S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 52.9 kDa
• Summary: This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. In both human and mouse, this gene has substitutions at several residues within the ATP binding motifs that in other kinases have been shown to be required for catalysis. In vitro assays indicate the protein lacks phosphorylation activity. The protein, however, likely retains its substrate binding capability. This gene is widely expressed in human tissues and its protein localizes to the nucleus. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).