TLE 1 (TLE1) (NM_005077) Human Untagged Clone

SKU SC310123

TLE1 (untagged)-Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TLE 1
• Synonyms: ESG; ESG1; GRG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005077 edited; ACCGCTGTGACTACTCTGGACTCGCCCGCAGAGTTTGCCCGCCGGGGAGGGTGGCCGATT; GGCGGAGCGCACTCCTGCTGTTTTCCCCACCATCTCGTGGATGTCGTCCCTCGGGTGGTG; AGAGAACTTTGCAGTGGGCTGGAGCGCCCTTTGCGGAGAAGCACACGAAGGTGAAGGAAG; GAAAGAAAGAAGACCGAGGAAGAAGGAGCTCGGAAGAAGGGTCCGGAGCGGCCGGTCCGG; CCGTGCAGGGCGAGTGCGCCCGAGGCGCGGCGCCCTGATGCTCCCCGGGCTCGAGGAGCA; TTGGGCACTTGCGGGACTGCTCTCGGTGGAGTACCNCCGTCGGCCCGGAAGGGGTTTGTG; CAACCGCGGAGACNACCGAGTGCTGGTCGACGGGGGGGCGTGCCGAGCCGCCTCCCGGCG; CGCCCTCCGCACTTTCCCCGCCTCGTCATCCGTCGCTCCCCGGCCAGGAGCCTCCGCTGC; GTCTTCAACCTCGCTCCCCTTTGCGTCCCGGGAGCCTGCGAGCACCCGGCCGAAGGCGCA; GCCGAATCTTGCGGGAGTCGCCCCGAAAGCGTCGGGTTTGTGTGGGGTTAGCGGGGGCCG; CCGCGCCACCTGCACCTCGCCCGCCGCCGCCTCGGGGAAAGCCCGAAGAGGAGGCGGACC; AGGAGAAGAGCAAAGAAAAGCAGTCCGTCTGGATTTGTTTGCCCAGGACTGGCGCCGCGC; ACGCGGATCGCCGAGGGGAGTGCGGTCGGAGTCACCGCGCCCCCGCCTCCCCGCCCGGGC; AGCTGAGGCCGGGGGTTGGAGCGCTGCCCCCGCGCACAGTCCCCGAGCGCCCGACGTCTC; CGCGCAGGTTCTTGAAGCAGCTGGGCCTGGGGCGCCCACTAATGTGGCCCTGAGGGCCGG; AGCCCGCACCGACGGGAGCGGGAGCCGGAGCAGCTGCGGGCGCCGAGTGGCCGGTGCGCC; CGGCGGAGCGCGCGTGCGTGGCCAGCGCGCTCCCCGCTTCTGCTTGGCTTTCCGGCTTAA; TTTTCCTCGGCGGGATTAAAGTTGGAAATTGACCGGAGAATTGAGTTGCCGGGGAACAGA; GCCCCGGCCGCCGCCAGAGCGATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCT; GCAGGCCAGCCCTTCAAGTTCACTATCCCGGAGTCCCTGGACCGGATTAAAGAGGAATTC; CAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATGTGAGAAACTGGCAAGTGAA; AAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGATTAAACATT; GAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATC; CCATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAG; GTGACCATGGCAGAATTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTT; TCTCATGGCCACGGACCCCCAGTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCT; GGAATCCCGCCCCTCGGGGGCAGTGCCGGCCTTCTTGCGCTGTCTAGTGCTCTGAGTGGG; CAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCACGATGCAGAGCACCACAGAGAC; AGAGAGCCGGGCACAAGTAATTCCCTCCTGGTCCCAGACAGTCTAAGAGGCACAGATAAA; CGCAGAAATGGACCTGAATTTTCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGAC; TCCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAACTTAGTTGTGGATGTGTCT; AATGAGGACCCTTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAAAATGGAATC; GACAAAAATCGCCTGCTAAAGAAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCG; GCAAGTTCCACTTCTTTGAAATCCAAAGAAATGAGCTTGCATGAAAAAGCCAGCACGCCT; GTTCTGAAATCCAGCACACCAACGCCTCGGAGCGACATGCCAACGCCGGGCACCAGCGCC; ACTCCAGGCCTCCGTCCAGGTCTCGGCAAGCCTCCAGCCATAGACCCCCTCGTTAACCAA; GCGGCAGCTGGCTTGAGGACACCCCTGGCAGTGCCCGGCCCATATCCTGCTCCTTTTGGG; ATGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCCAGGCGCTGCCTACGCCAGT; TTACACAACATGTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTGGTGGCCTAC; GGGCGCTCCCCCATGGTGGGGTTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCT; CCAAACCTGGCAGGAATCCCTGGGGGGAAACCTGCATACTCCTTCCACGTTACTGCAGAC; GGTCAGATGCAGCCTGTCCCTTTTCCCCCCGACGCCCTCATCGGACCCGGAATCCCCCGG; CATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATCAGC; AACCCCACGAGACACGTGTACACAGGCGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGC; CACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCTGAACAGAGACAATTATATC; CGTTCCTGTAAATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAAGCCAGTACT; TTGTCCATTTGGGACCTGGCGGCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCG; GCCCCCGCCTGCTACGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGC; AGCGACGGCAACATCGCTGTGTGGGATCTGCACAACCAGACACTAGTGAGGCAATTCCAG; GGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACG; GGTGGTTTGGACAACACAGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAG; CACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCCCACCGGGGAGTGGCTGGCA; GTGGGCATGGAGAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTACCAG; CTGCACCTGCATGAGAGCTGCGTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTT; GTGAGTACTGGAAAAGATAACCTCCTCAATGCTTGGCGGACCCCCTATGGAGCCAGCATA; TTCCAGTCCAAAGAGTCCTCGTCAGTGCTTAGCTGTGACATCTCTGTGGATGATAAGTAC; ATAGTCACTGGCTCGGGGGACAAGAAGGCTACAGTCTATGAAGTCATCTACTGAAAACAT; TATGTGGTTTAACGTTTATAGTTGAATTGGGCCAAAATGTTTCGAATTTATAGAAATAGA; AAAGTTGTAACTTTAAAAGAGAAAAAAAATTACAAACACCTGTTTCCAAACCTTGACAGA; AAACTACTTTGAGTCTACAAAGAGGAGGCGACAAGTCCATCAGCAGAAAGTCACCTGTCT; ACATAGACCAAATGGAGCACCAAGGCCAAGCGGACAGAGGGGCCATGGGTTGTAGGATTG; AGGAACGGAATCTGCCGACTCACATGACAGCCCATTCTTTCTTTCTGGGTGATCTGGGGA; TCACGCCTTGCCCAAGTGTGAGATTACCTTTCTGTTCCTTGCAGTTCACCTCACTTTCCG; TCCTTTGTAGAGCAGTGGTGTCTCCAATGAACTTGTTTCCTGGTTTTGCATCTTGTGAAA; TGTTTTTTTGTATTTTTGTTGAAGGTTAAACATTTGTATAAATTGTAAATATATTTGGTT; TATTACAGTAAAGGCTTTAGTACCAATAAAAAAAAAAAAAAAAAAAA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005077 unedited; NTGGGNCTTAGCTGCTTGTAACATTGCGGCCTTGGCGCCCACTATGTGCCCCTGAGGCCG; GAGCCCGCACCGACGGGAGCGGGAGCCGTGACAGCTGCGGGCGCCTAGTGGCCGGTGCTC; CCGGCGGAGCGCGCGTGCGTGGCCAGCGCGCTCCCCGCTTCTGCTTGGCTTTCCGGCTTA; ATTTTCCTCGGCGGGATTAAAGTTGGAAATTGACCGGATAATTGTGTTGCCGGGGAACAG; AGCCCCGGCCGCCGCCAGAGCGATGTTCCCGCAGAGCCGGCTCCCGACGCCGCACCATGC; TGCAGGCCAGCCCTTCAAGTTCACTATCCTGGATTCCCTGGACCGGATTAAAAGAGAATT; CCAGTTCCTGCGGGCGCAGTATCACATCCTTTAAATTGGAATGTGAGAAACTGGGCTGTC; GACAGACTGAACTGCTCAGCCCTATGTGATGGTTTATGAAATGTCATATTGGATTAACCA; TTGGAATGCACAACTAGACTTTTATCGTCTAGCAGTTTAATCCCATTTTTTTCCCATGTA; TTCACTTATTTTGTTTTATGAACTTTATAGCCAGGTGGCCCCAGACTATTTTTCGTCCCA; AGCAGTGTTCCAAGGCTTGATTTGAATCCTTGCTTCGTCCATAGAAAAATTCATATCTAT; GATTTGATCTCTTTGGTAGTGGCGCCCATTTTTCCTTTTTACACCTATTTCTATGAGAGT; CTTCTACTCCTATAAGATAAACACTTACATTATGGAGTGATGTATCCAGGNAATATATCT; AATCGTCTACTATCATTCTCGTTCACCAGCATACTCCCCCTCGCCTGACCCATGTTATAT; GTCAGGAGCACCCTTTTGTTTGTCCTCTATATATGTGCATGGTTAGTATTAACTACAAAA; TAACAATCGCATACATACCCAACCAATAAATACT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005077 unedited; TTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTATTGGTACTAAAGCCTTTACTGTAATAAA; CCAAATATATTTACAATTTATACAAATGTTTAACCTTCAACAAAAATACAAAAAAACATT; TCACAAGATGCAAAACCAGGAAACAAGTTCATTGGAGACACCACTGCTCTACAAAGGACG; GAAAGTGAGGTGAACTGCAAGGAACAGAAAGGTAATCTCACACTTGGGCAAGGCGTGATC; CCCAGATCACCCAGAAAGAAAGAATGGGCTGTCATGTGAGTCGGCAGATTCCGTTCCTCA; ATCCTACAACCCATGGCCCCTCTGTCCGCTTGGCCTTGGTGCTCCATTTGGTCTATGTAG; ACAGGTGACTTTCTGCTGATGGACTTGTCGCCTCCTCTTTGTAGACTCAAAGTAGTTTTC; TGTCAAGGTTTGGAAACAGGCGTTTGTAATTTTTTTTCTCTTTTAAAGTTACAACTTTTC; TATTTCTATAAATTCGAAACATTTTGGCCCAATTCAACTATAAACGTTAAACCACATAAT; GTTTTCAGTAGATGACTTCATAGACTGTAGCCTTCTTGTCCCCCGAGCCAGTGACTATGT; ACTTATCATCCACAGAGATGTCACAGCTAACCACTGACGAGGACTTTTTGAACCTGAATA; TGCTGGCTCCATAAGGGGTCCCCCCACCATTGAAGACGTTATTCTTTCCAGTCCTCCAAA; CCATTTACCCCAGAAGGCAATTTCAGGGACAGCCCGCCCTTTCATGCCGGTGCACTGGGA; CTTCCAAGCTTGTTAACTGCAAAACCTCCAATGGTGTTTTCTGCCAATGCCACCACTCCC; GGTGGGCATACCCCGTGAAAAACTTGGAGGGATTCTGCTTCCGCCCGGCCCCCTCCCGAG; GCCCAGACCGGACTGTGTTCAAACAACGTGCAGACTGGGGCCACATTTAAATCCATACAC; GTGTTCCTTTGCTGCCCCGCTAATTTTTATTTN;
• Restriction Sites: NotI-NotI
• ACCN: NM_005077
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005077.3, NP_005068.2
• RefSeq Size: 3283 bp
• RefSeq ORF: 2313 bp
• Locus ID: 7088
• UniProt ID: Q04724
• Cytogenetics: 9q21.32
• Domains: TLE_N, WD40
• Protein Families: Transcription Factors
• Summary: Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1.