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TRPC3 (NM_003305) Human Untagged Clone

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SKU
SC310083
TRPC3 (untagged)-Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2
  $777.00
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol TRPC3
Synonyms SCA41; TRP3
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>OriGene sequence for NM_003305 edited
CGTCTCTCTGTGGTTCAGTCACATTTGGGGTAAAACTGGAACCGAAGCCCTATAGGGTCC
CCTGAGGGAGAGAGTGGGCATCGGGCAGCCGGGGAACCGCTTTAGTGATAACTTTGACTG
CGGTTCCCAGGCGGTGCAGGGTCAGCGGCTCGCGCCCCGGCGCGCGGGCTCTCTTTTTCT
CTTTCTTTCTTTCCCTCTCTCTCTTTCTAAACGAAGGAGCGGTCGGCGCGCTAATGCATG
AGAAAGAGCAACTTTTCTTAATTTCAATCTTGGAGACAGCTTTTAATCAGTGCGAGACGC
AGAGAAGATGAAAAGAGGGTGATCGGTTTCACAGGGTCCATGGAGGGAAGCCCATCCCTG
AGACGCATGACAGTGATGCGGGAGAAGGGCCGGCGCCAGGCTGTCAGGGGCCCGGCCTTC
ATGTTCAATGACCGCGGCACCAGCCTCACCGCCGAGGAGGAGCGCTTCCTCGACGCCGCC
GAGTACGGCAACATCCCAGTGGTGCGCAAGATGCTGGAGGAGTCCAAGACGCTGAACGTC
AACTGCGTGGACTACATGGGCCAGAACGCGCTGCAGCTGGCTGTGGGCAACGAGCACCTG
GAGGTGACCGAGCTGCTGCTCAAGAAGGAGAACCTGGCGCGCATTGGCGACGCCCTGCTG
CTCGCCATCAGCAAGGGCTACGTGCGCATCGTAGAGGCCATCCTCAACCACCCTGGCTTC
GCGGCCAGCAAGCGTCTCACTCTGAGCCCCTGTGAGCAGGAGCTGCAGGACGACGACTTC
TACGCTTACGACGAGGACGGCACGCGCTTCTCGCCGGACATCACCCCCATCATCCTGGCG
GCGCACTGCCAGAAATACGAAGTGGTGCACATGCTGCTGATGAAGGGTGCCAGGATCGAG
CGGCCGCACGACTATTTCTGCAAGTGCGGGGACTGCATGGAGAAGCAGAGGCACGACTCC
TTCAGCCACTCACGCTCGAGGATCAATGCCTACAAGGGGCTGGCCAGCCCGGCTTACCTC
TCATTGTCCAGCGAGGACCCGGTGCTTACGGCCCTAGAGCTCAGCAACGAGCTGGCCAAG
CTGGCCAACATAGAGAAGGAGTTCAAGAATGACTATCGGAAGCTCTCCATGCAATGCAAA
GACTTTGTAGTGGGTGTGCTGGATCTCTGCCGAGACTCAGAAGAGGTAGAAGCCATTCTG
AATGGAGATCTGGAATCAGCAGAGCCTCTGGAGGTACACAGGCACAAAGCTTCATTAAGT
CGTGTCAAACTTGCCATTAAGTATGAAGTCAAAAAGTTTGTGGCTCATCCCAACTGCCAG
CAGCAGCTCTTGACGATCTGGTATGAGAACCTCTCAGGCCTAAGGGAGCAGACCATAGCT
ATCAAGTGTCTCGTTGTGCTGGTCGTGGCCCTGGGCCTTCCATTCCTGGCCATTGGCTAC
TGGATCGCACCTTGCAGCAGGCTGGGGAAAATTCTGCGAAGCCCTTTTATGAAGTTTGTA
GCACATGCAGCTTCTTTCATCATCTTCCTGGGTCTGCTTGTGTTCAATGCCTCAGACAGG
TTCGAAGGCATCACCACGCTGCCCAATATCACAGTTACTGACTATCCCAAACAGATCTTC
AGGGTGAAAACCACCCAGTTTACATGGACTGAAATGCTAATTATGGTCTGGGTTCTTGGA
ATGATGTGGTCTGAATGTAAAGAGCTCTGGCTGGAAGGACCTAGGGAATACATTTTGCAG
TTGTGGAATGTGCTTGACTTTGGGATGCTGTCCATCTTCATTGCTGCTTTCACAGCCAGA
TTCCTAGCTTTCCTTCAGGCAACGAAGGCACAACAGTATGTGGACAGTTACGTCCAAGAG
AGTGACCTCAGTGAAGTGACACTCCCACCAGAGATACAGTATTTCACTTATGCTAGAGAT
AAATGGCTCCCTTCTGACCCTCAGATTATATCTGAAGGCCTTTATGCCATAGCTGTTGTG
CTCAGCTTCTCTCGGATTGCGTACATCCTCCCTGCAAATGAGAGCTTTGGCCCCCTGCAG
ATCTCTCTTGGAAGGACTGTAAAGGACATATTCAAGTTCATGGTCCTCTTTATTATGGTG
TTTTTTGCCTTTATGATTGGCATGTTCATACTTTATTCTTACTACCTTGGGGCTAAAGTT
AATGCTGCTTTTACCACTGTAGAAGAAAGTTTCAAGACTTTATTTTGGTCAATATTTGGG
TTGTCTGAAGTGACTTCCGTTGTGCTCAAATATGATCACAAATTCATAGAAAATATTGGA
TACGTTCTTTATGGAATATACAATGTAACTATGGTGGTCGTTTTACTCAACATGCTAATT
GCTATGATTAATAGCTCATATCAAGAAATTGAGGATGACAGTGATGTAGAATGGAAGTTT
GCTCGTTCAAAACTTTGGTTATCCTATTTTGATGATGGAAAAACATTACCTCCACCTTTC
AGTCTAGTTCCTAGTCCAAAATCATTTGTTTATTTCATCATGCGAATTGTTAACTTTCCC
AAATGCAGAAGGAGAAGGCTTCAGAAGGATATAGAAATGGGAATGGGTAACTCAAAGTCC
AGGTTAAACCTCTTCACTCAGTCTAACTCAAGAGTTTTTGAATCACACAGTTTTAACAGC
ATTCTCAATCAGCCAACACGTTATCAGCAGATAATGAAAAGACTTATAAAGCGGTATGTT
TTGAAAGCACAAGTAGACAAAGAAAATGATGAAGTTAATGAAGGTGAATTAAAAGAAATC
AAGCAAGATATCTCCAGCCTTCGTTATGAACTTTTGGAAGACAAGAGCCAAGCAACTGAG
GAATTAGCCATTCTAATTCATAAACTTAGTGAGAAACTGAATCCCAGCATGCTGAGATGT
GAATGATACAGCAACCTGGATTTGGCTTTGACTATAGCACAAATGTGGGCAATAATATTT
CTAAGTATGAAATACTTGAAAAACTATGATGTAAATTTTTAGTATTAACTACCTTTATCA
TGTGAACCTTTAAAAGTTAGCTCTTAATGGTTTTATTGTTTTATCACATGAAAATGCATT
TTATTTGTCTGCTTTGACATTACAGTGGCATACCATTGTGTTGAAAAGCCCAATATTACT
ATATTATTGAAACTTTTATTCATTTTAGAGTAAACTCCACATCTTTGCACTACCTGTTTG
CCTCCAAGAGACTATCAGTTCCTTGGGGACAGGGACCATGTCTTATTCATCTTTGTGTCT
CCAGCATCTAGTACAGTGCCTGGTATATAGTAGGTGCTCAATAAATGTTGAAACCAACTG
AACTGCCAACAAAATAAAAATAAAAAGTCTTCACTATGTAGCATACCTTCCCTTGTCCAA
GTTCTGAAGAGGTTTTTTTTTTTTTTTTTAATAGAAACTGAAGACATTTTACAACCAGCT
ATGACTTGGTAAGACATTCTTAGAATTTTAGGTGTCACTGATAATCCTAGAACCACTGAG
CCCCAAGTGAAGAATTTAACAACAAAATGGGTTAATGAAAAATATAATTACATTGTATAT
TTAAGTTTCATAGAATTATTTAAAACAACACATTAAAGATTTTTCTAAAATATAAAAAAA
AAAAAAAAAAAAA
5' Read Nucleotide Sequence
>OriGene 5' read for NM_003305 unedited
GGTACACCTTGTATACGACTCCTATAGGGCGGCCGCGATCACGGCACGAGCCGTCTCTCT
GTGGTTCAGTCACACCCGGGGTAAAACTGGAACCGAAGCCCTATAGGGTCCCCTGAGGGA
GAGAGTGGGCATCGGGCAGCCGGGGAACCGCTTTAGTGATAACTTTGACTGCGGTTCCCA
GGCGGTGCAGGGTCAGCGGCTCGCGCCCCGGCGCGCGGGCTCTCTTTTTCTCTTTCTTTC
TTTCCCTCTCTCTCTTTCTAAACGAAGGAGCGGTCGGCGCGCTAATGCATGAGAAAGAGC
AACTTTTCTTAATTTCCAATCTTGGAGACAGCTTTTAATCAGTGCGAGACGCAGAGAAGA
TGAAAAGAGGGTGATCGGTTTCACAGGGTCCATGGAGGGAAGCCCATCCCTGAGACGCAT
GACAGTGATGCGGGAGAAGGGCCGGCGCCAGGCTGTCAGGGGCCCGGCCTTCATGTTCAA
TGACCGCGGCACCAGCCTCACCGCCGAGGAGGAGCGCTTCCTCGACGCCGCCGAGTACGG
CAACATCCCAGTGGTGCGCAAGATGCTGGAGGAGTCCAAGACGCTGAACGTCAACTGCGT
GGACTACATGGGCCAGAACGCGCTGCAGCTGGCTGTGGGCAACGAGCACCTGGAGGTGAC
CGAGCTGCTGCTCAAGAAGGAGAACCTGGCGCGCATTGGCGACGCCCTGCTGCTCGCCAT
CAGCAAGGGCTACGTGCGCATCGTAGAGGCCATCCTCAACCACCCTGGCTTCGCGGCCAG
CAAGCGTCTCACTCTGAGCCCCTGTGAGCAGAGCTGCAGACGACGACTTCTACGCTTACG
ACGAGGACGCACGCGCTCTCGCCGGACATCACCCCCATCATCCTGCGGCGCACTGCAGAA
TACGAAGTGGTGCACATGCTGCTGATGAA
3' Read Nucleotide Sequence
>OriGene 3' genomic read for NM_003305 unedited
TCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTATAT
TTTAGAAAAATCTTTAATGTGTTGTTTTAAATAATTCTATGAAACTTAAATATACAATGT
AATTATATTTTTCATTAACCCATTTTGTTGTTAAATTCTTCACTTGGGGCTCAGTGGTTC
TAGGATTATCAGTGACACCTAAAATTCTAAGAATGTCTTACCAAGTCATAGCTGGTTGTA
AAATGTCTTCAGTTTCTATTAAAAAAAAAAAAAAAAACCTCTTCAGAACTTGGACAAGGG
AAGGTATGCTACATAGTGAAGACTTTTTATTTTTATTTTGTTGGCAGTTCAGTTGGTTTC
AACATTTATTGAGCACCTACTATATACCAGGCACTGTACTAGATGCTGGAGACACAAAGA
TGAATAAGACATGGTCCCTGTCCCCAAGGAACTGATAGTCTCTTGGAGGCAAACAGGTAG
TGCAAAGATGTGGAGTTTACTCTAAAATGAATAAAAGTTTCAATAATATAGTAATATTGG
GCTTTTCAACACAATGGTATGCCACTGTAATGTCAAAGCAGACAAATAAAATGCATTTTC
ATGTGATAAAACAATAAAACCATTAAGAGCTAACTTTTAAAGGTTCACA
Restriction Sites NotI-NotI |
ACCN NM_003305
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_003305.1, NP_003296.1
RefSeq Size 3448 bp
RefSeq ORF 2547 bp
Locus ID 7222
UniProt ID Q13507
Cytogenetics 4q27
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
Summary The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Oct 2011
Transcript Variant: This variant (2) lacks an upstream exon , compared to variant 1, resulting in a different 5' UTR and 5' coding region and translation initiation from an inframe, downstream ATG. The encoded protein (isoform b) has a shorter N-terminus, compared to isoform a.
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"NM_003305" in other vectors (6)
SKU Description Size Price
RC210794 TRPC3 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2 10 ug
$776.00
RC210794L1 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2, Myc-DDK-tagged 10 ug
$1,076.00
RC210794L2 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2, mGFP tagged 10 ug
$1,076.00
RC210794L3 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2, Myc-DDK-tagged 10 ug
$1,076.00
RC210794L4 Lenti ORF clone of Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2, mGFP tagged 10 ug
$1,076.00
RG210794 TRPC3 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2 10 ug
$789.00 $976.00

file-alt Citations

TRPC3 and TRPC6 are essential for normal mechanotransduction in subsets of sensory neurons and cochlear hair cells
Quick, Zhao, Eijkelkamp et al
Open Biol (2012) 2 (5), 120068 DOI: 10.1098/rsob.120068
Cited by 169
AI Summary: In this paper, OriGene products were used to facilitate the study of TRPC3 and TRPC6 channels, specifically utilizing OriGene’s TRPC3 and TRPC6 expression plasmids to co-express these channels in sensory neuron cell lines and assess their role in mechanotransduction.
FigureFigureFigure
Showing 1-1 of 1 papers.
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