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NDST3 (NM_004784) Human Untagged Clone

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SKU
SC310066
NDST3 (untagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3)
  $800.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Untagged Clone
Target Symbol NDST3
Synonyms HSST3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
Fully Sequenced ORF
>SC310066 representing NM_004784.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCGGCCGCGAATTCGGCACGAGGCTCCCGTCCTCAGCTCCCGCTCCCTCCCCTTCCCCGGGGCGGGCGC
GCGCGGGCCGGGAGCTGGGAGGCGGCGGCTCCCGCCCGGGAGGCGGTGGCGAGGGCGGCGGAGGCGCGG
CCGGGCGGGTCCCAGGGCCGTGGTTCTCCAGCGCAGGAGTCCCCGGCGCACCCGCTGCGCTCGCCCCGC
AGCCGCAGCCTGAGCGGCCGAAGTCGCCACCAGCTGTGTCCGCGCAGATCCCGGGCGCGGAGGGGAGAG
GTCGCCCGCTTGTCGCCCCCGCCGGACCCCGGGCACCGCGTAGCACCCAAGCGCCACCCGGACGAGGGG
CCCTGGGGAGCGGCTGAGGCCGAAGACCCGAGGGAAGGAGGAGCCCGCGGACGGCTGCAGCCCCAGACT
GTATTTTCTGTGAGTCCTGATCAAGTGATACAAATGAGCTGCAATGGTGACATAAACTCTTGACAGAGA
TTGGAAAAGTAGCTGGAACACCATCTTTTCTTTTAACTTTTTATGGTGCTTCTGTTGGCATAGTTGGGG
AAAGCACCTACAACATGAGTTTTATCATGAAGCTTCACAGACACTTTCAAAGAACAGTCATTCTGCTTG
CCACTTTTTGTATGGTGAGCATTATCATTTCTGCTTACTACCTGTACAGTGGCTACAAACAGGAAAATG
AACTCTCTGAGACGGCTTCAGAAGTTGACTGTGGCGACCTCCAACACCTACCATATCAACTAATGGAAG
TGAAAGCAATGAAGCTTTTTGATGCCTCAAGGACAGACCCCACAGTCCTAGTATTTGTAGAGAGCCAGT
ACTCATCTCTTGGTCAAGACATCATTATGATTCTAGAATCAAGTAGATTCCAGTATCACATTGAAATTG
CCCCTGGAAAGGGAGATCTCCCAGTGCTTATAGACAAAATGAAAGGCAAATACATTCTCATTATTTATG
AGAATATTTTAAAGTATATAAATATGGATTCCTGGAATCGAAGCCTTCTAGATAAATACTGTGTAGAAT
ATGGTGTGGGTGTCATTGGATTCCACAAAACTAGTGAGAAGAGTGTACAGAGCTTTCAGTTAAAAGGTT
TCCCTTTTTCCATATATGGAAATCTTGCAGTAAAAGATTGTTGTATTAATCCTCATTCTCCATTGATTC
GTGTGACCAAATCTTCCAAGCTTGAAAAAGGTTCTTTACCTGGAACTGACTGGACAGTTTTTCAGATTA
ATCATTCAGCCTATCAACCAGTAATATTTGCCAAAGTAAAGACCCCAGAAAACCTTTCTCCTTCCATCT
CTAAAGGTGCTTTTTATGCCACTATTATACATGACCTGGGGCTTCATGATGGAATTCAAAGGGTTCTTT
TTGGCAACAACTTGAACTTTTGGCTGCACAAGCTCATCTTCATAGATGCCATCTCCTTCTTATCAGGGA
AGAGGCTGACATTGTCCTTGGACAGGTACATTCTTGTGGATATTGATGATATATTTGTGGGAAAAGAGG
GAACAAGAATGAACACCAATGATGTAAAGGCCCTGCTTGATACTCAGAATCTTTTGCGTGCACAAATCA
CAAATTTTACATTCAACCTGGGATTTTCAGGGAAATTTTACCATACAGGAACTGAAGAGGAAGATGAAG
GAGATGACTGTCTGTTGGGGTCTGTGGATGAGTTCTGGTGTTTTCCTCACATGTGGAGCCATATGCAGC
CCCACCTCTTCCACAATGAGTCATCTTTGGTGGAGCAGATGATTCTCAACAAAAAATTTGCCTTAGAGC
ACGGCATTCCAACGGACATGGGCTACGCTGTGGCCCCTCACCATTCGGGCGTCTACCCTGTACATGTTC
AGCTTTACGAGGCCTGGAAGAAGGTCTGGAATATTAAAATCACCAGCACTGAAGAATATCCACATCTGA
AGCCAGCTAGATACCGGAGGGGTTTTATCCACAAAAACATCATGGTTCTCCCAAGACAAACCTGTGGGC
TTTTCACTCACACCATTTTCTACAAAGAATATCCAGGGGGTCCTAAAGAGCTGGATAAGAGTATCCAAG
GAGGAGAACTTTTCTTCACTGTCGTCCTCAACCCTATCAGCATTTTCATGACCCATTTGTCCAACTATG
GGAATGACCGACTGGGATTATATACATTTGTTAATCTGGCCAACTTTGTGAAGAGCTGGACCAACCTGC
GACTTCAGACTCTGCCTCCAGTACAACTGGCCCACAAGTATTTTGAGCTGTTTCCTGATCAGAAAGACC
CTCTCTGGCAGAATCCTTGCGATGACAAACGCCACAGAGACATTTGGTCTAAAGAAAAAACTTGTGATC
GCTTACCAAAATTCTTGGTAATAGGACCCCAGAAAACTGGTACCACTGCTTTGTATTTGTTCCTGGTTA
TGCATCCTTCCATCCTTAGTAACTCCCCCAGCCCAAAAACCTTTGAGGAGGTACAGTTCTTTAATAGAA
ATAACTACCACAGGGGGATTGATTGGTATATGGATTTCTTCCCAGTCCCATCTAATGTCACTACCGACT
TTTTGTTTGAGAAGAGTGCCAATTACTTCCACTCAGAGGAAGCCCCTAAAAGAGCTGCTTCTCTGGTTC
CCAAAGCCAAGATTATCACCATTCTCATTGACCCTTCAGACCGAGCATACTCCTGGTACCAGCATCAGC
GATCACATGAAGATCCTGCAGCTCTGAAGTTTAGCTTCTACGAAGTGATCTCAGCAGGGCCCCGTGCAC
CCTCGGAGCTCAGAGCCTTGCAGAAGAGATGTTTGGTCCCGGGGTGGTATGCCAGCCACATCGAGAGAT
GGCTTGTTTATTTCCCCCCATTTCAGTTGCTAATTATTGATGGGCAACAACTAAGAACTGATCCTGCTA
CAGTGATGGATGAAGTACAGAAGTTTCTAGGAGTCTTGCCTCATTATAATTACTCAGAAGCTTTAACGT
TTGATTCTCATAAAGGTTTCTGGTGTCAGTTACTGGAAGAAGGTAAAACAAAATGCCTTGGAAAGAGCA
AAGGAAGAAAATACCCTCCAATGGATTCTGATAGCAGGACATTTCTGTCAAGCTACTATCGAGATCACA
ACGTGGAACTCTCAAAGCTGCTGCACAAACTGGGTCAGCCTCTGCCATCCTGGCTGAGACAGGAGCTGC
AGAAAGTAAGATAGCACTGAGAGAAAACTTGAGACTTCATCGTCCATGTAGAACACACCTTTTCCAAAG
CTTCCAGAAGCTACCAAAAGGCAGTTGAAAAATATACCTCTTCAAATGAGAAAAAAGAACAGTTTCTTC
CATGTGCTGGCATGTGGATGATTAGAAAAAAAGAAAAAGTATGTGTTACAAGCCTTGAGGCCTGTGGCC
TTTCTCTTAACCCATATCTGAGCCTGTGGGATTATTGTAGACTACTGTGCACTCATGTGGAAGTCAATT
GCAACCAACATAAATATCAAACACAAATGCAGAACTGTTCCATTTCATAGTAATATTTACACTTTTATA
TATCAACTAAGATTGTGTCTCTGTAGGTTTTTAGACCACTGTTTGCCTGTACGATGTTTTCTTATTATT
TTATTCTATAAAGTGTCCTATGAAACAAGAAGCAAAATAAACATCACAATGTAGCATAAAATGTCAAAA
GCATAACACCCATGAAAAATGCTTGCCAAAATTTAAATCCACTTAAGTATTTAGAAATAATTGTAAATT
ATAATTTGTGCTAGATCAGAGTGGAAAAGTTATATATGAGTGTTATCTGCACCAAAAAAAAATAAGTGC
TATAGCAAAAGTGATTTTTTCATATACATAGTCTAGTGAATATATGTAAAAATTACTAACTCTTCCATC
ACAACGTAATTGTTATACTGAAATATATACTGTAAAGCTGTTGGATGGTAAATAGAAACACATCAAGTA
CACCAAAACAGTAAATAACAATGTAAAGTGTACTGACTGAATCCTCTAAAAGGGAGAAATTGTAGGGGT
CTAAAAACATATCATCGAGAATTCTATGTCCACTTGCTGTAAGCTGTGAATCTATCCCAGTAGTCCTCA
CTAGTTCTACAGAAATGCATGCCTAGAGTCTGAGGTATGTGTCATCTCCAGAAACAGGTTGACTTTTAA
ATGTGAATAGCTATTTTTAATGCTCCCCAAAACAAAACCTATTAAACTAAAACTAAAAACAGTTTCCCT
AGATGATGTGGTTAGGCTATTTACATTTAGGTTATTAAAAAATTGCATTTTCCCAGATAGTTCAAAATA
AAATTATGTAACGTAGTAGAGATTTATAACAAAAAACAAATTAATATATTATTTGTAATCTAATGTTTG
CAATGATACTTTTGATATAAGGCAAAGAAACATAAGCAGAGCAGAGGCCCATTTTTGAAAATATTTCAC
CCATCACTCTGGTGGAAGTAACAGAATCATAGGTACAATTAAGGATTAGCTACTAAATTAAATGCAGGA
AGTACAAAGATGAGCAAGTCATGCTCTTGAAATATATTTACATTTCAATTGTAAATAATATATATGGTT
AATAAAGTTTCCAAAAAAGAAAAGAATACATCATACTTATTTTCCTGTTAATATTTACTTAATTTTTTT
TTTGAGAGGGGGACAGAGTCTCACTATGTCACCCAGGCTGGAGTGCAATGGTGTGATCTCAGCTCACTG
CAACCTCCGCCTCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCTGAGGAGCTGGGATTACAGCCAT
GCACCACCACGCCCGGGTAATTTTTGTATTTTTAGTAGTGACAGGGTTTCACCATGTTGACTAGGCTGA
TCTTGAACTCCTGACCTCATGATCCACCCACCTTGGCCTCTCAAAGTGCTGAGATTACAAGCGTGAGCC
ACCATGCCCTGACTACTTAATTTTTAAATGTACTGTACATATACATAAAATTTAAGTAGTAAATTAATT
CCCTGCTATGATTATAAGCCTTTATAAAATATTATCAAAGGTATGATTAGAAACAAATGGGAGGAAAAC
CCATAGTAAAAATATTTTCATTGTCATTTTAATGGGTAAAACTATCAATTACTAATTAGAAAATGAATT
GTGAGATCCACTTACTATAAATTTTTAACTGTACATTATAATTACTTTTTTAAAGTTTCATGTACCTAA
AGCATATGCTAATTTTTAAATATACTATTAGCAGAAAAAATTGGAAAAATCACCAATTTTTAAGACTCT
TGACTCTAAAAGCAAAATCAAAGTGATTTGGACAAATTTGGGAAACACAGTCTTCATGCTTACTGCTGT
CTCTTTTATAGCAGCAGATGCCAGACATGCTAGGAAAATTACTATAGTCTTCCATCTCCACCAACTTAA
GAAAACTGGCTTAGGAAGGTTAAACATGAGAATTGGCCTCAGCAGTTATATACAGTGTCATGTTTTCAT
TTGGCAACAACATTTATTCTAATAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC
5' Read Nucleotide Sequence
>OriGene 5' read for NM_004784 unedited
AACCGGCCGTTGAGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG
CTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTC
GGCACGAGGCTCCCGTCCTCAGCTCCCGCTCCCTCCCCTTCCCCGGGGCGGGCGCGCGCG
GGCCGGGAGCTGGGAGGCGGCGGCTCCCGCCCGGGAGGCGGTGGCGAGGGCGGCGGAGGC
GCGGCCGGGCGGGTCCCAGGGCCGTGGTTCTCCAGCGCAGGAGTCCCCGGCGCACCCGCT
GCGCTCGCCCCGCAGCCGCAGCCTGAGCGGCCGAAGTCGCCACCAGCTGTGTCCGCGCAG
ATCCCGGGCGCGGAGGGGAGAGGTCGCCCGCTTGTCGCCCCCGCCGGACCCCGGGCACCG
CGTAGCACCCAAGCGCCACCCGGACGAGGGGCCCTGGGGAGCGGCTGAGGCCGAAGACCC
GAGGGAAGGAGGAGCCCGCGGACGGCTGCAGCCCCAGACTGTATTTTCTGTGAGTCCTGA
TCAAGTGATACAAATGAGCTGCAATGGTGACATAAACTCTTGACAGAGATTGGAAAAGTA
GCTGGAACACCATCTTTTCTTTTAACTTTTTATGGTGCTTCTGTTGGCATAGTTGGGGAA
AGCACCTACAACATGAGTTTTATCATGAAGCTTCACAGACACTTTCAAAGAACAGTCATT
CTGCTTGCCACTTTTTGTATGGTGAGCATTATCATTTCTGCTTACTACCTGTACAGTGGC
TACAAACAGGAAAATGAACTCTCTGAGACGGCTTCAGAAGTTGACTGTGGCGACCTCAAC
ACCTACCATATCAACTAATGGAAGTGAAAGCAATGAGCTTTTTGATGCCTCAGAAGACCC
CACAGTCCTAGTATTTTGTAGAGAAGCCAGTACTCCATCCTTCTTGGTCAGAACATCCA
3' Read Nucleotide Sequence
>Forward primer walk for NM_004784 unedited
TGCCCTCTTATAATTCTCGAGCTTTAACGTTTGATTCTCATAAAGGTTTCTGGTGTCAGT
TACTGGAAGAAGGTAAAACAAAATGCCTTGGAAAGAGCAAAGGAAGAAAATACCCTCCAA
TGGATTCTGATAGCAGGACATTTCTGTCAAGCTACTATCGAGATCACAACGTGGAACTCT
CAAAGCTGCTGCACAAACTGGGTCAGCCTCTGCCATCCTGGCTGAGACAGGAGCTGCAGA
AAGTAAGATAGCACTGAGAGAAAACTTGAGACTTCATCGTCCATGTAGAACACACCTTTT
CCAAAGCTTCCAGAAGCTACCAAAAGGCAGTTGAAAAATATACCTCTTCAAATGAGAAAA
AAGAACAGTTTCTTCCATGTGCTGGCATGTGGATGATTAGAAAAAAAGAAAAAGTATGTG
TTACAAGCCTTGAGGCCTGTGGCCTTTCTCTTAACCCATATCTGAGCCTGTGGGATTATT
GTAGACTACTGTGCACTCATGTGGAAGTCAATTGCAACCAACATAAATATCAAACACAAA
TGCAGAACTGTTCCATTTCATAGTAATATTTACACTTTTATATATCAACTAAGATTGTGT
CTCTGTAGGTTTTTAGACCACTGTTTGCCTGTACGATGTTTTCTTATTATTTTATTCTAT
AAAGTGTCCTATGAAACAAGAAGCAAAATAAACATCACAATGTAGCATAAAATGTCAAAA
GCATAACACCCATGAAAAATGCTTGCCAAAATTTAAATCCACTTAAGTATTTAGAAATAA
TTGTAAATTATAATTTGTGCTAGATCAGAGTGGAAAAGTTATATATGAGTGTTATCTGCA
CCAAAAAAAAATAAGTGCTATAGCAAAGTGATTTTTCATATACATAGTCTAGTGAATATA
TTGTAAAAATTACTAACTTCTTCCATCACAACGTAATTGTTATAACTGGATT
Restriction Sites SgfI-MluI |
ACCN NM_004784
Insert Size 5513 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to differ from the protein associated to this reference by a single amino acid.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004784.1
RefSeq Size 3188 bp
RefSeq ORF 5513 bp
Locus ID 9348
UniProt ID O95803
Cytogenetics 4q26
Domains Sulfotransfer
Protein Families Transmembrane
Protein Pathways Heparan sulfate biosynthesis, Metabolic pathways
MW 211.5 kDa
Summary This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. This monomeric bifunctional enzyme catalyzes the N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin, which are the initial chemical modifications required for the biosynthesis of the functional oligosaccharide sequences that define the specific ligand binding activities of heparan sulfate and heparin. provided by RefSeq, Nov 2008
Transcript Variant: This variant (1) represents the protein-coding transcript.
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"NM_004784" in other vectors (6)
SKU Description Size Price
RC217551 NDST3 (Myc-DDK-tagged)-Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3) 10 ug
$799.00
RC217551L1 Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3), Myc-DDK-tagged 10 ug
$1,099.00
RC217551L2 Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3), mGFP tagged 10 ug
$1,099.00
RC217551L3 Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3), Myc-DDK-tagged 10 ug
$1,099.00
RC217551L4 Lenti ORF clone of Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3), mGFP tagged 10 ug
$1,099.00
RG217551 NDST3 (tGFP-tagged) - Human N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3) 10 ug
$789.00 $999.00

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